RESULTS
The bacterial growth is determined by medium turbidity and gas production (bubbles on the surface, close to the parrafin oil film).
The bacterial growth is determined by medium turbidity and gas production (bubbles on the surface, close to the parrafin oil film).
DESCRIPTION
Enrichment medium for the cultivation of Gram-positive anaerobic sporogenic
bacteria (Clostridium and related species).
COMPOSITION
Nutrient broth with pieces of liver or fat-free minced cooked meat of ox heart, 0.5–1%
glucose and casein hydrolysate.
PROCEDURE
The medium should be heated before inoculation for 10-15 minutes and rapidly
cooled, in order to remove the oxygen. After inoculation, the medium will be covered
with a film of paraffin oil or vaseline. The inoculated culture is reheated at 80°C for 20
minutes to kill non-spore-forming bacteria.
Enrichment medium for the cultivation of Gram-positive anaerobic sporogenic
bacteria (Clostridium and related species).
COMPOSITION
Nutrient broth with pieces of liver or fat-free minced cooked meat of ox heart, 0.5–1%
glucose and casein hydrolysate.
PROCEDURE
The medium should be heated before inoculation for 10-15 minutes and rapidly
cooled, in order to remove the oxygen. After inoculation, the medium will be covered
with a film of paraffin oil or vaseline. The inoculated culture is reheated at 80°C for 20
minutes to kill non-spore-forming bacteria.
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| Kitt-Tarozzi medium before innoculation |
