PROCEDURE
Inoculate the culture medium by streaking the plate. Incubate at 30 - 37 ºC for 24 hours.
If germs are in small quantity the material can be enriched in starch broth (peptone
from meat 6.25 g, starch 2.5 g, sodium chloride 1.5, bromthymol blue 0.5 ml of
aqueous 1.6% solution; water ad 100 ml; pH 7.2)
When examining water, the Aeromonas titre can also be determined by adding 100 ml
of the water sample to 60 ml starch broth.
RESULTS
Aeromonas colonies are convex, moist and red whereas Pseudomonas & Enterobacteriaceae remains colourless (dextrin is not
degraded). Dextrin-degrading Enterobacteriaceae also form red colonies but do not exhibit a purple coloration after adding Nadi
reagent (aqueous 1% solution of NNN’N’-tetramethyl-p-phenylen-diammonium dichloride)
Pseudomonas do not degrade dextrin, do not display a Nadi reaction and produce a deep blue coloration after adding Nadi reagent.
REFERENCES
Inoculate the culture medium by streaking the plate. Incubate at 30 - 37 ºC for 24 hours.
If germs are in small quantity the material can be enriched in starch broth (peptone
from meat 6.25 g, starch 2.5 g, sodium chloride 1.5, bromthymol blue 0.5 ml of
aqueous 1.6% solution; water ad 100 ml; pH 7.2)
When examining water, the Aeromonas titre can also be determined by adding 100 ml
of the water sample to 60 ml starch broth.
RESULTS
Aeromonas colonies are convex, moist and red whereas Pseudomonas & Enterobacteriaceae remains colourless (dextrin is not
degraded). Dextrin-degrading Enterobacteriaceae also form red colonies but do not exhibit a purple coloration after adding Nadi
reagent (aqueous 1% solution of NNN’N’-tetramethyl-p-phenylen-diammonium dichloride)
Pseudomonas do not degrade dextrin, do not display a Nadi reaction and produce a deep blue coloration after adding Nadi reagent.
REFERENCES
- Mc Kracken A.W. & Barkley R. : Isolation of Aeromonas species from clinical sources. J.Clin. Pathol., 25; 970-975, 1972.
- Handbook Culture Media Merck, 1982.
- Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie
clinica ed. III, 2009, 1045-1084.
COMPOSITION
Peptone from casein 10.0 g, meat extract 3.0 g, sodium chloride 5.0 g, dextrin 15 g,
sodium sulfite 1.6 g, fuchsin 0.25 g, di-sodium hydrogen phosphate 7.75 g, agar-agar
13.0 g.
Distilled water ad 1000 ml, pH 7.5 ± 0.2, sterilize by autoclaving.
Adjust medium colour by adding a few drops of sodium sulfite solution .
Peptone from casein 10.0 g, meat extract 3.0 g, sodium chloride 5.0 g, dextrin 15 g,
sodium sulfite 1.6 g, fuchsin 0.25 g, di-sodium hydrogen phosphate 7.75 g, agar-agar
13.0 g.
Distilled water ad 1000 ml, pH 7.5 ± 0.2, sterilize by autoclaving.
Adjust medium colour by adding a few drops of sodium sulfite solution .
DESCRIPTION
Medium for the isolation of Aeromonas and the differentiation from Pseudomonas and Enterobacteriaceae species.
Medium for the isolation of Aeromonas and the differentiation from Pseudomonas and Enterobacteriaceae species.
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