| Coagulated serum (left), liquefaction after 24h incubation (middle) and after 48h (right) |
| Liquefaction of coagulated serum |
MEDIUM PREPARATION
Harvest horse or bovine blood in a sterile jar or flask, closed with cotton plug. Place
on ice for 24-48 hours, during which time coagulation has taken place; the serum
may then be siphoned off.
The serum is distributed in sterile tubes, then coagulated at a temperature of 75-
80° C. in the Koch coagulator (any form of sterilizer may, however, be used). Place
the tubes in an inclined position. The essentials are that the temperature should be
raised very gradually. After coagulation, the medium acquires a grayish-white color.
PROCEDURE
Innoculate the coagulated serum and incubate at 37 °C for 24-48 hours. Result is
positive if the coagulated serum switch to liquid state partially or totally.
Harvest horse or bovine blood in a sterile jar or flask, closed with cotton plug. Place
on ice for 24-48 hours, during which time coagulation has taken place; the serum
may then be siphoned off.
The serum is distributed in sterile tubes, then coagulated at a temperature of 75-
80° C. in the Koch coagulator (any form of sterilizer may, however, be used). Place
the tubes in an inclined position. The essentials are that the temperature should be
raised very gradually. After coagulation, the medium acquires a grayish-white color.
PROCEDURE
Innoculate the coagulated serum and incubate at 37 °C for 24-48 hours. Result is
positive if the coagulated serum switch to liquid state partially or totally.
(c) Costin Stoica
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DESCRIPTION
Some fastidious pathogenic bacteria, especially from the nose and throat can produce enzymes that hydrolyse proteins. Coagulated
serum or the Loeffler medium (which contains a high amount of serum) enhances primary and secondary isolation and cultivation of
these microorganisms and reveal their proteolytic activity.
Some fastidious pathogenic bacteria, especially from the nose and throat can produce enzymes that hydrolyse proteins. Coagulated
serum or the Loeffler medium (which contains a high amount of serum) enhances primary and secondary isolation and cultivation of
these microorganisms and reveal their proteolytic activity.
