Acidovorax (Pseudomonas) facilis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas facilis
 Davis 1969 (Hydrogenomonas facilis Schatz and Bovell 1952)

Moved to Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Comamonadaceae, Genus Acidovorax,

Acidovorax facilis
Willems et al. 1990.
Gram-negative rods, 0.2-0.3 x 1.2-2.8 μm, motile by one or two polar flagella.
Strictly aerobic, optimal temperature 28 ºC. No growth at 41 ºC. No fluorescein
production. Diffusible or cellular pigments are not produced. Organic growth factors
are not required. Autotrophic growth occurs in atmosphere containing H
2, O2 & CO2.
Can use many organic compounds as sole carbon source for heterotrophic growth.
Grows on nutritive agar, trypticase Soy Agar, christensen (urease negative),
heterotrophic medium for Hydrogenomonas (KH
2PO4  0.2 g, MgSO4  0.1 g, Sodium
citrate x 2H
2O  0.5 g, Sodium acetate x 3H2O  0.3 g, Sodium succinate x 6H2O .2.0 g,
Sodium glutamate 1.0 g, Agar 15.0 g, Tryptose 5.0 g, Yeast extract  1.0 g, Corn starch
2.0 g, Distilled water.1.0 L). Hemolysis not produced.
Isolated from soil by enrichments in mineral media and atmosphere with H2, O2 & CO2.
Unknown (none).
  1. Schatz A. & Bovell C.R.: Growth and hydrogenase activity of a new bacterium, Hydrogenomonas facilis. Journal of Bacteriology,
    1952, 63, 87-98.
  2. Davis D.H., Doudoroff M., Stanier R.Y. & Mandel M.: Proposal to reject the genus Hydrogenomonas: taxonomic implications.
    International Journal of Systematic Bacteriology, 1969, 19, 375-390.
  3. Willems A., Falsen E., Pot B., Jantzen E., Hoste B., Vandamme P., Gillis M., Kersters K. & De Ley J.: Acidovorax, a new genus for
    Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the
    species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov. Int. J. Syst.
    Bacteriol., 1990, 40, 384-398.
  4. Anne Willems and Monique Gillis: Family IV. Comamonadaceae Willems, De Ley, Gillis and Kersters 1991a, 447VP. In Bergey’s
    Manual of Systematic Bacteriology Second Edition Volume Two The Proteobacteria Part C The Alpha-, Beta-, Delta-, and
    Epsilonproteobacteria, George M. Garrity Editor-in-Chief, 2005, pp 686-759.
Positive results for catalase, gelatin hydrolysis & oxidase.
Can utilize: D-ribose, L-arabinose, malonate, mannitol, beta-alanine, L-serine, L-leucine, L-histidine, D-glucose, glycerol, butyrate,
succinate, suberate, azelate, sebacate, D-malate, 3-hydroxybutyrate, L-proline & L-glutamate.

Negative for DNase, production of H
2S in TSI; hydrolysis of esculin, denitrification, lysine and ornithine decarboxylases; arginine
dihydrolase; beta-galactosidase; growth on cetrimide; indole production; Tween 80, acid production in 10% lactose, in TSI medium,
and in oxidative-fermentative medium containing D-glucose, D-fructose, D-xylose, maltose, or adonitol.
No utilization of: adipate, propionate, n-valerate, trehalose, cellobiose, citrate, mesaconate, ethanol, para-hydroxybenzoate,
L-tryptophan, nicotinate, erythritol, D-arabinose, D-xylose, L-xylose, L-sorbose, L-rhamnose, adonitol, dulcitol, inositol, xylitol,
L-arabitol, methyl-D-xyloside, methyl-D-mannoside, methyl-D-glucoside, N-acetylglucosamine, D-cellobiose, maltose, lactose,
D-melibiose, sucrose, trehalose, beta-gentiobiose, D-rnelezitose, D-raffinose, D-turanose, D-lyxose, D-tagatose, L-fucose,
amygdalin, esculin, salicin, arbutin, inulin, starch, glycogen, 5-ketogluconate, glycolate, caprylate, pelargonate, caprate, oxalate,
malonate, L-tartrate, itaconate, mesaconate, phenylacetate, benzoate, ohydroxybenzoate, D-mandelate, L-mandelate, isophthalate,
terephthalate, glycine, L-cysteine, D-tryptophan, L-lysine, L-arginine, trigonelline, DL-kynurenine, betaine, creatine, ethylamine,
butylamine, amylamine, ethanolamine, benzylamine, diaminobutane, urea, acetamide, sarcosine, spermine, histamine, tryptamine,
and glucosamine.
(c) Costin Stoica
Culture media
Biochemical tests
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