Pseudomonas (Comamonas / Delftia)  acidovorans
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas acidovorans den Dooren de Jong 1926.
Pseudomonas indoloxidans Gray 1928; Pseudomonas desmolytica Gray and Thornton 1928; Comamonas acidovorans
(den Dooren de Jong 1926) Tamaoka et al. 1987;
Delftia acidovorans (den Dooren de Jong 1926) Wen et al. 1999
Gram-negative rods with polar or bipolar flagella, 0.8-1.1 by 2.5-4.1 μm. Poly-beta-
hydroxybutyrate is accumulated in cells.
Nonhemolytic, nonpigmented, 2-4 mm diameter colonies. Strains designated P.
produce insoluble blue pigment, indigotin, in media containing indole
and a suitable carbon source. Obligately aerobic, 30 ºC (no growth at 4 or 41 ºC).
Media: Trypticase Soy Agar  ± 5% sheep blood; Trypticase Soy Broth.
Isolated from soil, water (pisciculture farms) & sludge of biological wastewater treatment plants.
Generally, is a non-pathogenic bacteria. Reports of human catheter-associated bacteremia & endocarditis. Possible fish pathogen.
  1. R.E. Buchanan & N.E. Gibbons: Bergey’s Manual of Determinative Bacteriology, eighth edition, 232.
  2. Willems A., Pot B., Falsen E., Vandamme P., Gillis M., Kersters K. & De Ley J.: Polyphasic taxonomic study of the emended genus
    Comamonas: relationship to Aquaspirillum aquaticum, E. Falsen group 10, and other clinical isolates. Int. J. Syst. Bacteriol., 1991,
    41, 427-444.
  3. Wen A., Fegan M., Hayward C., Chakraborty S. & Sly L.I.: Phylogenetic relationships among members of the Comamonadaceae,
    and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov. Int. J. Syst.
    Bacteriol., 1999, 49, 567-576.
  4. H. Horowitz, S Gilroy, S. Feinstein, and G. Gilardi: Endocarditis associated with Comamonas acidovorans. J Clin Microbiol. 1990
    January; 28(1): 143–145.
Positive results for catalase, denitrification & oxidase.
Can utilize: acetate, aconitate, adipate,  6-aminovalerate, L-aspartate, azelate, butanol, 2,3-butylene glycol, butyrate, citraconate,
ethanol, D-fructose, fumarate, gluconate, L-glutamate, glutarate, glycerate, glycolate, hippurate, L-histidine, m-hydroxybenzoate,
p-hydroxybenzoate, beta-hydroxybutyrate, hydroxymethylglutarate, isobutyrate,  isovalerate, itaconate, alpha-ketoglutarate, kynurenate,
L-kynurenine, lactate, levulinate, L-leucine,  L-malate, mannitol, mesaconate, mucate, nicotinate,  DL-norvaline, pimelate, L-proline,
n-propanol, propionate, pyruvate, quinate, saccharate, sebacate, suberate, succinate, m-tartrate, L-tyrosine & valerate.

Negative results for arginine dihydrolase, DNase, H
2S production, indole production, gelatin hydrolysis, starch hydrolysis, urease,
esculin (beta glucosidase) &  ONPG (beta-galactosidase).
No utilization of: N-acetylglucosamine, adonitol, 3-aminobenzoate, amylamine, anthranilate, D- and L-arabinose, D- and L-arabitol,
arbutin, L-arginine, benzoylformate, benzylamine, betaine, butylaminei caprylate, cellobiose, L-citrulline, creatine, L-cysteine,
diaminobutane, dodecane, dulcitol, aesculin, erythritol, ethanolamine, ethylamine, D-galactose, beta-gentiobiose, glucosamine,
geraniol, D-glucose, methyl alpha-D-glucoside, glycogen, heptanoate, hexadecane, histamine, o-hydroxybenzoate,
poly-beta-hydroxybutyrate, DL-beta-hydroxybutyrate, inulin, isophthalate, 2- and 5-ketogluconate, lactose, L-lysine, D-lyxose, maltose,
D-mandelate, D-mannose, methyl-alpha-D-mannoside, D-melezitose, D-melibiose, naphthalene, L-ornithine, oxalate, pantothenate,
phenol, phenylethanediol, putrescine, L-rhamnose, D-raffinose, D-ribose, salicin, L-serine, sorbitol, L-sorbose, spermine, sucrose,
starch, D-(-)-tartrate, terephthalate, testosterone, D-turanose, trehalose, tryptamine, urea, m-xylitol, D- and L-xylose & methyl

Variable utilization of: citrate, m-inositol, acetamide, L- and D-alanine, 2-aminobutyrate, caproate, glycine , L-isoleucine, D-malate,
maleate, malonate, DL-norleucine, L-norleucine, phenylacetate, L-phenylalanine, L-(+)-tartrate, trigonelline, D- and L-tryptophan,
benzoate, ethylene glycol, D-fucose, L-mandelate, pelargonate, phthalate, propylene glycol, sarcosine, L-theronine, L-valine,
beta-alanine,2-aminobenzoate, 4-aminobenzoate, 3-aminobutyrate, 4-aminobutyrate, 5-aminobutyrate, alpha-aminovalerate,
amygdalin, 2,3-butylene glycol, caprate, glycerol, m-erythritol, heptanoate, isobutanol, DL-kynurenine, L-methionine & tagatose.
(c) Costin Stoica
Culture media
Biochemical tests
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