Mycobacterium holsaticum
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinomycetota, Class Actinomycetes, Order Mycobacteriales, Family Mycobacteriaceae, Genus Mycobacterium,
Mycobacterium holsaticum
Richter et al. 2002.
Acid-alcohol-fast, short or coccoid rods.
Colonies are dysgonic and transparent at 22 ºC, but smooth, moist, shiny and
off-white- to yellow-pigmented at higher temperatures. Isolates grow within 7 days at
temperatures up to 40 ºC; higher temperatures seem to favor growth. No growth at 45
ºC. Growth occurs on media supplemented with 5% (w/v) NaCl, but not on MacConkey
agar (without crystal violet) .
Isolated from clinical specimens (sputum, urine, gastric fluid). Resistant to  thiophene-2-carboxylic acid hydrazide (2 µg/ml), isoniazid
and rifampin, but susceptible to streptomycin, ethambutol, protionamide and ofloxacin.
Undetermined.
  1. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  2. Richter E, Niemann S, Gloeckner FO, Pfyffer GE, Rüsch-Gerdes S. Mycobacterium holsaticum sp. nov. Int J Syst Evol Microbiol.
    2002;52(Pt 6):1991-1996. doi:10.1099/00207713-52-6-1991
Positive results for acid phosphatase, nicotinamidase, pyrazinamidase, nitrate reduction, Tween 80 hydrolysis (weak reaction), and
urease.

Negative results for arylsulfatase, acetamidase, benzamidase, allantoinamidase, succinamidase, heat-stable catalase, iron uptake,
tellurite reduction (3 days).
(c) Costin Stoica
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