Mycobacterium florentinum
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
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Phylum Actinomycetota, Class Actinomycetes, Order Mycobacteriales, Family Mycobacteriaceae, Genus Mycobacterium,
Mycobacterium florentinum
Tortoli et al. 2005.

Member of the
Mycobacterium simiae complex.
Acid-fast rods. Non-motile. Non-capsulated. Non-spore-forming. Gram-positive.
Colonies on Lowenstein-Jensen medium are smooth, creamy, and non-chromogenic
and appear after 2 weeks incubation. Grows at 25-37 ºC, but not at 42 or 45 ºC. Does
not grow on MacConkey agar or on media supplemented with 5% (w/v) NaCl.
Isolated from human clinical samples (seven from sputum, and one from lymphnode).
Resistant to isoniazid (1 μg/ml), hydroxylamine, oleic acid, p-nitrobenzoic acid, thiacetazone and ciprofloxacin. Variable results for
amikacin, ethambutol, and rifampicin.
Isolated from a patient with pulmonary infection.
  1. John G. Magee and Alan C. Ward 2012. Family III. Mycobacteriaceae Chester 1897, 63AL in Bergey’s Manual of Systematic
    Bacteriology, Volume Five The Actinobacteria, Part A, Michael Goodfellow & al. (editors), 312-375.
  2. Tortoli E, Rindi L, Goh KS, Katila ML, Mariottini A, Mattei R, Mazzarelli G, Suomalainen S, Torkko P, Rastogi N. Mycobacterium
    florentinum sp. nov., isolated from humans. Int J Syst Evol Microbiol 2005; 55:1101-1106.
Positive results for tellurite reduction (9 days) and urea hydrolysis.

Negative results for arylsulphatase (3, 7 and 10 days), niacin accumulation, and Tween 80 hydrolysis.

Variable results for catalase, semiquantitative catalase test, and nitrate reduction.
(c) Costin Stoica
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