| Escherichia ruysiae |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Enterobacterales, Family Enterobacteriaceae,
Genus Escherichia, Escherichia ruysiae van der Putten et al. 2021.
Genus Escherichia, Escherichia ruysiae van der Putten et al. 2021.
Gram-negative rods, 1.0 x 2.0 μm. Non-spore-forming. Non-motile.
Colonies are convex, grey-white and semitransparent on Columbia sheep blood agar
incubated overnight at 37 ºC. Grows at/in: 20-42 ºC and 0-6% NaCl. Facultatively
anaerobic.
incubated overnight at 37 ºC. Grows at/in: 20-42 ºC and 0-6% NaCl. Facultatively
anaerobic.
Isolated from human (faecal material of an international traveller returning from Asia, a diarrheal stool sample of an adult woman, a
stool sample from a healthy individual living in India, urine and other), wild animal (wild boars, rat, mouse, sea gull, urban crows,
capybara and other) poultry (chicken), livestock (swine), companion animal (dog) and environment (water, soil, river sediment).
Sensible to cefpodoxime, enrofloxacin, chloramphenicol, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, gentamicin.
Resistant to clindamycin, erythromycin, metronidazole. Intermediate to amoxicillin-clavulanate.
stool sample from a healthy individual living in India, urine and other), wild animal (wild boars, rat, mouse, sea gull, urban crows,
capybara and other) poultry (chicken), livestock (swine), companion animal (dog) and environment (water, soil, river sediment).
Sensible to cefpodoxime, enrofloxacin, chloramphenicol, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, gentamicin.
Resistant to clindamycin, erythromycin, metronidazole. Intermediate to amoxicillin-clavulanate.
Several virulence factor genes were identified, including those encoding colonization factors (fimH), fitness (iutA, kpsMII), toxins (pic,
sat, estB, astA) and effectors (aaiC).
May acquire clinically important Antibiotic Resistance Genes and transmit them to other clinically significant bacteria.
May have pathogenic and zoonotic potential.
sat, estB, astA) and effectors (aaiC).
May acquire clinically important Antibiotic Resistance Genes and transmit them to other clinically significant bacteria.
May have pathogenic and zoonotic potential.
- van der Putten BC, Matamoros S, Mende DR, Scholl ER, COMBAT consortium and Schultsz C, 2021. Escherichia ruysiae sp. nov.,
a novel Gram-stain-negative bacterium, isolated from a faecal sample of an international traveller. Int J Syst Evol Microbiol 71:
004609. - Campos-Madueno EI, Aldeia C, Sendi P, Endimiani A, 2023. Escherichia ruysiae may serve as a reservoir of antibiotic resistance
genes across multiple settings and regions. Microbiology Spectrum 11(4). Doi: 10.1128/spectrum.01753-23. - Sakaguchi K., Tanabe M., Takizawa S, Kasahara S. et al., 2023. Zoonotic potential and antimicrobial resistance of Escherichia
spp. in urban crows in Japan-first detection of E. marmotae and E. ruysiae. Comparative Immunology, Microbiology and Infectious
Diseases 100, 102040. - Siddi G., Piras F., Gymoese P., Torpdahl M. et al., 2024. Pathogenic profile and antimicrobial resistance of Escherichia coli,
Escherichia marmotae and Escherichia ruysiae detected from hunted wild boars in Sardinia. International Journal of Food
Microbiology 16, 421. - Dione N., Mlaga K.D., Liang S., Jospin G. et. al., 2025. Comparative genomic and phenotypic description of Escherichia ruysiae: a
newly identified member of the gut microbiome of the domestic dog. Front. Microbiol. 16:1558802.
Positive results for beta-galactosidase, beta-glucuronidase, leucine arylamidase, naphthol-AS-BI-phosphohydrolase, ornithine
decarboxylase, acid production from: L-arabinose, glucose, maltose, D-mannitol, D-mannose, rhamnose, D-sorbitol, and trehalose.
Negative results for arginine dihydrolase, citrate utilization, cystine arylamidase, esterase (C4), esterase-lipase (C8), alpha-
fucosidase, gelatinase, beta-glucosidase, H2S production, indole production, N-acetyl-glucosaminidase, lipase, alpha-mannosidase,
oxidase, trypsin, alpha-chymotrypsin, urease, valine arylamidase, beta- xylosidase, acid production from: amygdalinadonitol, L-
arabitol, cellobiose, 5-ketogluconate, D-melibiose, tagatose* and D-xylose.
No utilization of histidine, malonate, malate and lactate.
Variable results for acid and alkaline phosphatase, alpha-glucosidase, alpha-galactosidase, beta- glucuronidase, acid production
from sucrose*.
Note: *contradictions in original paper vs supplementary material.
decarboxylase, acid production from: L-arabinose, glucose, maltose, D-mannitol, D-mannose, rhamnose, D-sorbitol, and trehalose.
Negative results for arginine dihydrolase, citrate utilization, cystine arylamidase, esterase (C4), esterase-lipase (C8), alpha-
fucosidase, gelatinase, beta-glucosidase, H2S production, indole production, N-acetyl-glucosaminidase, lipase, alpha-mannosidase,
oxidase, trypsin, alpha-chymotrypsin, urease, valine arylamidase, beta- xylosidase, acid production from: amygdalinadonitol, L-
arabitol, cellobiose, 5-ketogluconate, D-melibiose, tagatose* and D-xylose.
No utilization of histidine, malonate, malate and lactate.
Variable results for acid and alkaline phosphatase, alpha-glucosidase, alpha-galactosidase, beta- glucuronidase, acid production
from sucrose*.
Note: *contradictions in original paper vs supplementary material.
(c) Costin Stoica
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