GENERAL
Improved interspecies comparison and filter functions in the "Analytics" menu and added "Duplicates and high imilarities finder" function. Optimization of the antibiogram interpreter, the identification engine, and Salmonella / Streptococcus antigenic search tools.
Added "Antibiogram" function in the Android app.

NEISSERIA DATABASE
Added Microvirgula curvata and emmended description of Microvirgula aerodenitrificans.

ENTEROBACTERIA DATABASE
Emended description of Escherichia marmotae.

PASTEURELLA DATABASE
Replacement of the illegitimate prokaryotic genus name Catenococcus by Allocatenococcus.

STAPHYLOCOCCUS DATABASE
Removal of the "colony size" test due to its low discriminatory value for identification and variability depending on the nutritional quality of the culture medium.

BACILLUS DATABASE
* Added 45 new species:
Alteribacter aurantiacus,
Anoxybacillus andreesenii,
Bacillus sonorensis, B.atrophaeus, B. vallismortis, B. mojavensis, B. pseudomycoides, B. wiedmannii, B. toyonensis, B. gaemokensis, B. manliponensis, B. gobiensis, B. halotolerans, B. kexueae, B. manusensis, B. marinisedimentorum, B. paramobilis, B. sanguinis, B. piscicola, B. rhizoplanae, B. salacetis, B. salis, B. salitolerans, B. salinus, B. suaedae, B. taeanensis, B. shivajii, B. glycinifermentans, B. oleivorans, B. solimangrovi, B. spongiae,
Cytobacillus luteolus, Cytobacillus kochii,
Ferdinandcohnia humi,
Litchfieldia alkalitelluris,
Metabacillus litoralis,
Mesobacillus zeae,
Oikeobacillus pervagus,
Paenibacillus aurantiacus,
Paenibacillus agaridevorans,
Polygonibacillus indicireducens,
Rossellomorea oryzaecorticis,
Sutcliffiella halmapala,
Viridibacillus arvi and Viridibacillus arenosi.
* Emended description of 60 other species.

GENERAL CHANGES
The ABIS online Android app update to version 2.0.0 is now available on Google Play.
Taxonomic changes at the phylum level: Firmicutes => Bacillota, Proteobacteria => Pseudomonadota, Actinobacteria => Actinomycetota. The genus Gemella finally received a family, moving from "incertae sedis" to Gemellaceae.

BACILLUS DATABASE
* Added 38 new species: Alkalicoccus chagannorensis, Bacillus albus, B. aquiflavi, B. australimaris, B. capparidis, B. chungangensis, B. cytotoxicus, B. dafuensis, B. daqingensis, B. dicomae, B. ectoiniformans, B. enclensis, B. endozanthoxylicus (Niallia endozanthoxylica), B. fengqiuensis, B. fungorum, B. luti, B. mesophilus, B. mobilis, B. nitratireducens, B. oryzisoli (Niallia oryzisoli), B. paramycoides, B. proteolyticus, B. paranthracis, B. pacificus, B. seohaeanensis, B. songklensis, B. thaonhiensis, B. tropicus, B. xiamenensis, B. zhangzhouensis, Cytobacillus oceanisediminis, Hydrogenibacillus schlegelii, Metabacillus idriensis, Metabacillus herbersteinensis, Paenibacillus sonchi, Priestia abyssalis, Salipaludibacillus agaradhaerens and Shouchella clausii.
* Emended description of 16 other species.
* Bacillus firmus and B. horneckiae were moved to genus Cytobacillus.
* Alkalihalobacillus pseudofirmus was moved to genus Alkalihalophilus.
* B. bataviensis, B. drentensis, B. fumarioli, B. niacini, B. novalis, B. soli and B. vireti were moved to genus Neobacillus.
* B. clausii was moved to genus Shouchella.
* B. halodurans was moved to genus Halalkalibacterium. According to Joshi et al. 2021, B. okuhidensis is a later heterotypic synonym of Halalkalibacterium halodurans.
* B. jeotgali and B. subterraneus were moved to genus Mesobacillus.
* B. korlensis and B. siralis were moved to genus Robertmurraya.
* B. marisflavi and B. vietnamensis were moved to genus Rossellomorea.
* B. oleronius was moved to genus Heyndrickxia.
* B. schlegelii was moved to genus Hydrogenibacillus.
* B. plakortidis and B. lehensis are later heterotypic synonyms of Bacillus oshimensis.
* B. rhizosphaerae is a later heterotypic synonym of Shouchella clausii.
* P. riograndensis is a later heterotypic synonym of Paenibacillus sonchi.
* B. weihenstephanensis is a later heterotypic synonym of Bacillus mycoides.
* P. jamilae is a later heterotypic synonym of Paenibacillus polymyxa.

ENTEROBACTERIA DATABASE
* Emended description of Escherichia ruysiae.

BACILLUS DATABASE
* B. acidiceler and B. luciferensis were moved to genus Gottfriedia.
* B. endophyticus, B. flexus, B. koreensis and B. megaterium were moved to genus Priestia.
* B. asahii, B. muralis, B. psychrosaccharolyticus and B. simplex were moved to genus Peribacillus.
* B. sporothermodurans and B. shackletonii were moved to genus Heyndrickxia.
* B. farraginis, B fordii and B. fortis were moved to genus Siminovitchia.
* B. agaradhaerens and B. neihouensis were moved to genus Salipaludibacterium.
* B. krulwichiae and B. wakoensis were moved to genus Halalkalibacter.
* B. alcalophilus, B. algicola, B. alkalilacus, B. hemicentroti, B. macyae, B. pseudalcaliphilus, B. pseudofirmus and B. trypoxylicola were moved to genus Alkalihalobacillus.
* B. clarkii, B. polygoni and B. vedderi were moved to genus Evansella.
* B. funiculus was moved to genus Ectobacillus.
* B. azotoformans was moved to genus Schinkia.
* B. badius was moved to genus Pseudobacillus.
* B. decolorationis and B. hwajinpoensis were moved to genus Pseudalkalibacllus.
* B. selenitireducens was moved to genus Salisediminibacterium.
* B. cohnii, B. halmapalus and B. horikoshii were moved to genus Sutcliffiella.
* B. niacini and B. indicus were moved to genus Metabacilus. B. cibi is considered a synonym of B. indicus.
* B. galactosidilyticus, B. lentus and B. ruris were moved to genus Lederbergia.
* B. gibsonii was moved to genus Alkalicoccobacillus.

CAMPYLOBACTER DATABASE
* Added 24 new Arcobacter species: A. anaerophilus, A. antarcticus, A. aquimarinus, A. bivalviorum, A. canalis, A. caeni, A. cloacae, A. defluvii, A. ebronensis, A. ellisii, A. faecis, A. haliotis, A. lacus, A. lanthieri, A. lekithochrous, A. marinus, A. molluscorum, A. pacificus, A. parvus, A. suis, A. trophiarum, A. vandammei, A. venerupis and A. vitoriensis.

LISTERIA DATABASE
* Removed the "dextrin fermentation" test as it has low discrimination value.
* Added "acid" and "alkaline phosphatase" tests.
* Added 26 new species: Listeria marthii, L. cossartiae, L. immobilis, L. portnoyi, L. rustica, L. farberi, L. costaricensis, L. goaensis, L. thailandensis, L. valentina, Erysipelothrix amsterdamensis, E. piscisicarius, Turicibacter faecis, Holdemanella biformis, Allocoprobacillus halotolerans, Amedibacillus dolichus, Amedibacterium intestinale, Catenisphaera adipataccumulans, Copranaerobaculum intestinale, Dielma fastidiosa, Faecalibacillus faecis, F. intestinalis, Intestinibaculum porci, Longibaculum muris, Longicatena caecimuris and Tannockella kyphosi.

STREPTOCOCCUS DATABASE
* Transfer of Facklamia tabacinasalis to genus Ruoffia as Ruoffia tabacinasalis and emended description.
* Added 7 new species: Facklamia lactis, Faecalicoccus acidiformans, Faecalitalea cylindroides, Fundicoccus ignavus, Fundicoccus fermenti, Ruoffia halotolerans and Suicoccus acidiformans.

CAMPYLOBACTER DATABASE
* Added 23 new species: Campylobacter iguaniorum, C. corcagiensis, C. geochelonis, C. hepaticus, C. ornithocola, C. pinnipediorum, C. blaseri, C. armoricus, C. novaezeelandiae, C. massiliensis, C. portucalensis, C. bilis, C. magnus, C. troglodytis, C. californiensis, C. majalis, C. suis, C. devanensis, C. porcelli, C. vicugnae, C. molothri, C. felis and C. ovis.
* Emended description of Campylobacter fetus subsp. venerealis, Campylobacter lanienae, Campylobacter (Bacteroides) ureolyticus and Campylobacter sputorum biovar paraureolyticus.

PSEUDOMONAS DATABASE
* Added 4 Trinickia species: Trinickia dabaoshanensis, T. diaoshuihuensis, T. dinghuensis and T. fusca.
* Emended description of T. soli and T. symbiotica.
* Trehalose utilization test for Pseudomonas aeruginosa was changed to variable.

The ABIS microbial database reached the 3000 species milestone. The lucky 3000th taxon is Pseudomonas ogarae - a species isolated from the rhizosphere of sugar beet.

PSEUDOMONAS DATABASE
* Added 45 new species isolated from fish, milk, beef, insects, plants, soil and water: Pseudomonas caspiana, P. gregormendelii, P. guryensis, P. ullengensis, P. lactucae, P. lalucatii, P. mucoides, P. neuropathica, P. pisciculturae, P. piscium, P. nicosulfuronedens, P. ovata, P. paracarnis, P. paraversuta, P. quercus, P. saliphila, P. schmalbachii, P. saxonica, P. tohonis, P. typographi, P. aromaticivorans, P. benzopyrenica, P. boanensis, P. californiensis, P. quasicaspiana, P. canavaninivorans, P. crudilactis, P. cuatrocienegasensis, P. danubii, P. folii, P. triticifolii, P. germanica, P. aestiva, P. grandcourensis, P. helvetica, P. purpurea, P. knackmussii, P. lijiangensis, P. ogarae, P. oligotrophica, P. pergaminensis, P. quebecensis, P. rustica, P. serbiensis and Halopseudomonas salegens.
* Emended description of 34 older species.

ENTEROBACTERIA DATABASE
Enterobacter cloacae subsp. cloacae - urea hydrolysis was set negative.

STAPHYLOCOCCUS DATABASE
Minor adjustments for Staphylococcus xylosus and S. nepalensis.

BACILLUS DATABASE
Emended description of Bacillus thuringiensis.

GENERAL
* New Android app released. See the Google Play Store.
* A session variables expiration control was added to the 'Input'and 'Compare' pages.
* Removed a bug that was destroying the SESSION variables in the 'Contact' page.
* The login page can automatically detect a mobile device and redirect the user to a mobile-friendly page.
* Selected 'Target' is now automatically deleted when returning to main menu; however, red target indicator is still functional if duplicate pages are used in the browser. The use of multiple tabs is prohibited as it can seriously affect the identification results!
* The 'Results' page no longer displays taxa with a probability of less than 1%.
* Activity and status report: added the approximate geolocation for the 'World activity', showing 'people interest' and the result of the most 'recent identification'.

PSEUDOMONAS DATABASE
* Increasing the accuracy of identification by replacing the generic "Arabitol" and "Xylose" tests with the stereoisomers "D-Arabitol" and "D-Xylose".
* Added 14 new species: Pseudomonas thermotolerans, P. leptonychotis, P. lalkuanensis, P. resinovorans, P. rhizoryzae, P. viciae, P. xionganensis, P. yangonensis, P. allokribbensis, P. gozinkensis, P. glycinae, P. anatoliensis, P. iridis, P. arcuscaelestis, P. atacamensis, P. aylmerensis, P. baltica, P. kielensis, P. bijieensis, P. campi, P. capsici, P. carbonaria, P. cremoris and P. cyclaminis.
* Emended description of 22 older species.

STREPTOCOCCUS DATABASE
* Added 14 Lactococcus species: Lactococcus taiwanensis, L. formosensis, L. hircilactis, L. laudensis, L. nasutitermitis, L. petauri, L. reticulitermitis, L. termiticola, L. allomyrinae, L. carnosus, L. paracarnosus, L. hodotermopsidis, L. insecticola and L. kimchii.
* Emended description of 99 Streptococcus and Lactococcus species.
* According to Nouioui et al. 2018 Atopobium parvulum, the former Streptococcus parvulus, belongs to genus Lancefieldella as Lancefieldella parvula.
* Streptococcus oligofermentans is considered to be a later heterotypic synonym of Streptococcus cristatus.
* Li et al. 2021 transferred Lactococcus lactis subsp. cremoris to Lactococcus cremoris and Lactococcus lactis subsp. tructae to Lactococcus cremoris subsp. tructae.

ENTEROBACTERIA DATABASE
* Added Mixta intestinalis and Photorhabdus caribbeanensis.
* emended description of Musicola paradisiaca.

CORYNEBACTERIUM DATABASE
* Correction in the input page: 'growth at 45°C' was replaced by 'growth at 46°C'.

PASTEURELLACEAE DATABASE
* According to Bisgaard and Christensen (2023), Mannheimia pernigra is an earlier heterotypic synonym of Mannheimia ovis.
* According to Gaillot et al. (2013), Pasteurella lymphangitidis is a later heterotypic synonym of Yersinia pseudotuberculosis.
* Adhikary et al. (2017), proposed the transfer of Pasteurella pneumotropica to genus Rodentibacter as Rodentibacter pneumotropicus.
* Added 22 new species: Mannheimia bovis, M. pernigra, M. caviae, Necropsobacter rosorum, Nicoletella semolina, Otariodibacter oris, Rodentibacter caecimuris, R. heidelbergensis, R. mrazii, R. myodis, R. rarus, R. ratti, R. trehalosifermentans, R. haemolyticus, Seminibacterium arietis, Spirabiliibacterium falconis, S. mucosae, S. pneumoniae, Testudinibacter aquarius, Ursidibacter arcticus, U. maritimus and Vespertiliibacter pulmonis.
* Emended description of 34 older species.

BACILLUS DATABASE
* Added Bacillus velezensis.
* Emended description of Bacillus amyloliquefaciens.

PSEUDOMONAS DATABASE
* Psedomonas duriflava was moved to genus Chryseomonas.
* Pseudomonas saccharophila was moved to genus Roseateles as Roseateles saccharophilus.
* Pseudomonas stutzeri, P. azotoformans, P. balearica, P. xanthomarina and P. zhaodongensis were moved to genus Stutzerimonas.
* Burkholderia alpina was moved to genus Pararobbsia.
* Paraburkholderia caryophylii, P. soli and P. symbiotica were moved to genus Trinickia.
* Paraburkholderia endofungorum and P. rhizoxinica were moved to genus Mycetohabitans.
* According to Babich et al. (2023), Pusillimonas soli and Pusillimonas ginsengisoli belong to genus Allopusillimonas.
* Added 3 biochemical tests that improve the Pseudomonads identification: acid phosphatase activity, galactose utilization and 5-ketogluconate utilization.
* Added 29 new species: Bordetella pseudohinzii, Burkholderia ubonensis, Pararobbsia silviterrae, Pelistega ratti, Pseudomonas sichuanensis, P. tructae, P. bubulae, P. carnis, P. neustonica, P. defluvii, P. fildesensis, P. haemolytica, P. synxantha, P. hydrolytica, P. izuensis, P. kairouanensis, P. nabeulensis, P. piscis, P. karstica, P. spelaei, P. khazarica, Stutzerimonas kirkiae, S. balearica, S. nosocomialis, S. degradans, S. frequens, S. zhaodongensis, S. nitrititolerans, S. tarimensis and S. urumqiensis.
* Emended description of 130 other species.

LACTOBACILLUS DATABASE
* Added 4 new species from the genus Lacticaseibacillus: L. absianus, L. parahuelsenbergensis, L. styriensis and L. zeae subsp. silagei.
* Emended description of Lacticaseibacillus zeae subsp. zeae.

GENERAL CHANGES
* "Filters" button, a function that can help the user when there are not enough tests available, is now orange-highlighted when insufficient tests are provided for identification. Additionally, the user can now apply two different types of filters: "Coarse" which allows all strains meeting the criteria and "Fine" which removes variable and rarely meeting criteria strains.
* "Compare" button is now orange-highlighted when the discrimination between taxa is too low.
* A red "Target" button warns the user that the selected taxon does not belong to the current database (this function is not available in the mobile application).
* Added new functions in 'Activity and status report': 'people interest' (species that users are interested in) and 'recent findings' (the results of the most recent identifications).

GENERAL CHANGES
* Added the 'Inconsistency' function which shows conflicting tests (e.g. selecting positive gas production but negative glucose fermentation).
* Minor graphical changes and adjustments.

PSEUDOMONAS DATABASE
* Emended description of Pseudomonas putida and combined biovar A and B. Emended description of other 23 Pseudomonas species.
* According to Tohya et al. 2020 Pseudomonas fuscovaginae is a later heterotypic synonym of Pseudomonas asplenii. The two species were merged in ABIS database.
* Added 24 new species: P. alloputida, P. abietaniphila, P. asiatica, P. bohemica, P. daroniae, P. dryadis, P. edaphica, P. floridensis, P. furukawaii, P. inefficax, P. juntendi, P. laurylsulfatiphila, P. laurylsulfativorans, P. mangiferae, P. mangrovi, P. persica, P. profundi, Pseudomonas reidholzensis, P. sesami, P. silesiensis, P. tianjinensis, P. versuta, P. wadenswilerensis and Atopomonas hussainii.

PASTEURELLACEAE DATABASE
* According to Dickerman et al. 2020, Haemophilus parasuis was reclassified as Glaesserella parasuis.
* According to Christensen et al.2021, Haemophilus haemoglobinophilus was reclassified as Canicola haemoglobinophilus.
* Added 20 new species: Actinobacillus porcitonsillarum, A. anseriformium, A. vicugnae, Glaesserella australis, Avibacterium endocarditidis, Basfia succiniciproducens, Bisgaardia hudsonensis, Bisgaardia miroungae, Caviibacterium pharyngocola, Conservatibacter flavescens, Chelonobacter oris, Cricetibacter osteomyelitidis, Mesocricetibacter intestinalis, Frederiksenia canicola, Haemophilus pittmaniae, H. seminalis, H. sputorum, H. massiliensis, Histophilus somni and Lonepinella koalarum.
* Emended description of 17 other species.
* The cyclic AMP (AMPc) test was removed from the database as it has low discrimination power.

ENTEROBACTERIACEAE DATABASE
* Emended description of Escherichia marmotae and E.ruysiae.

PSEUDOMONAS DATABASE
* added 22 new species isolated from hot spring, soil, bark beetle, plants (Solanum tuberosum, Populus euphratica), sea sediment, compost and bovine milk: Pseudomonas guguanensis, P. punonensis, P. helmanticensis, P. prosekii, P. coleopterorum, P. endophytica, P. psychrophila, P. glareae, P. granadensis, P. matsuisoli, P. azotifigens, P. soli, P. yamanorum, P. helleri, P. weihenstephanensis, P. populi, P. turukhanskensis, P. vancouverensis, P. cerasi, P. orientalis, P. lactis and P. paralactis.
Some are psychrophilic, other are thermophilic and other are plant pathogens (P. cerasi).
*emended description of 30 older species.

LACTOBACILLUS DATABASE
* added 3 new species grass and corn silage, mustard and feces: Lacticaseibacillus huelsenbergensis, Ligilactobacilus cholophilus and Limosilactobacillus caccae.

ENTEROBACTERIA DATABASE
* added Leclercia tamurae.
* emended description of Leclercia adecarboxylata.

BACILLUS DATABASE
* minor adjustments of citrate utilization test for B. subtilis, B. thuringiensis, B. megaterium and B. cereus.

BIFIDOBACTERIUM DATABASE
* added Bifidobacterium longum subsp. iuvenis.

LACTOBACILLUS DATABASE
* Added 2 new species: Ligilactobacilus pabuli and Lactobacillus isalae.

BACILLUS DATABASE
* Added 3 more species: Paenibacillus azotofixans, Paenibacillus mucilaginosus and Paenibacillus edaphicus.

PSEUDOMONAS DATABASE
* Emended decription of Pseudomonas segetis, P. pohangensis, P. extremorientalis, P. azotoformans, P. poae and P. trivialis.
* Added 34 new species: Pseudomonas caricapapayae, P. alkylphenolica, P. arsenicoxydans, P. benzenivorans, P. saponiphila, P. canadensis, P. brassicae, P. huaxiensis, P. sivasensis, P. petroselini, P. extremaustralis, P. taeanensis, P. allii, P. kitaguniensis, P. chengduensis, P. donghuensis, P. toyotomiensis, P. taiwanensis, P. composti, P. deceptionensis, P. baetica, P. jessenii, P. mandelii, P. jessenii, P. entomophila, P. protegens, P. guangdongensis, P. guariconensis, P. sagittaria, P. linyingensis, P. kuykendallii, P. laurentiana, P. palmensis, P. asturiensis and P. seleniipraecipitans.

OTHER CHANGES
* Removed a bug that blocked the input field in Lancefield database.
* Correction of the probability calculation formula that disfavored the taxa with an incomplete matrix.
* For a better evaluation of the identification possibilities, the limitation of the displayed taxa in the results page was lowered from 80% to 75% similarity.

ENTEROBACTERALES DATABASE
* Revision of the members of genus Citrobacter.
* Emended description of Escherichia albertii, E. coli biotype 2, E. fergusonii, E. marmotae and E.ruysiae.
* Escherichia vulneris Brenner et al. 1983 was moved to genus Pseudescherichia as Pseudescherichia vulneris (Brenner et al. 1983) Alnajar and Gupta 2017.

PSEUDOMONAS DATABASE
* Added 30 new species isolated from plants, soil and deep sea samples: Chryseomonas asuensis, Denitrificimonas caeni, Entomomonas moraniae, Halopseudomonas aestusnigri , H. bauzanensis, H. litoralis, H. formosensis, H. pelagia, H. gallaeciensis, H. oceani, H. salina, H. phragmitis, H. xinjiangensis, Rhizobacter dauci, R. fulvus, R. bergeniae, R. gummiphilus, R. profundi, Rugamonas rubra, R. aquatica, R. rivuli, R violacea, Thiopseudomonas denitrificans, T. alkaliphila, Pseudomonas abyssi, P. aegrilactucae, P. akappageensis and P. atagonensis.
* Emended decription of Pseudomonas japonica and P. alcaliphila.

STREPTOCOCCUS DATABASE
* Refining of Enterococcus faecium and Enterococcus lactis biochemical identification data.

OTHER CHANGES
* Modification of the identification code and sorting of the results: the percentage of probability now has priority over the percentage of biochemical similarity.
* Graphical refining and optimizations.

NOTICE FOR ANDROID USERS
* The "ABIS bacteria identification" application (net.tgw1916.apk) is no longer available for installing from Google Play Store. You have to manually download and install the Android application from https://www.tgw1916.net/software.html

SOFTWARE CHANGES
* Added database filter option in "Analytics" page. "All of a kind" option has been removed.
* Added "Filter" and "Strongest" test determination buttons in "Input" and "Results" pages.
* Added a two taxa "Comparison" button and moved "Serology" and "Warning" buttons on the upper bar in "Results" page. "Serology" button works only in case of Salmonella or Streptococcus species detection and leads to Kauffmann-White-Le Minor and Lancefield classification schemes, respectively.

PASTEURELLACEAE DATABASE
* Added Coenonia anatina and Ornithobacterium rhinotracheale, two respiratory tract pathogens for birds.
* Emmended description of Mannheimia species.

PSEUDOMONAS DATABASE
* Added 4 birds pathogens (pigeons, ducks and geese): Riemerella columbipharyngis, Chryseobacterium gleum, Chryseobacterium balustinum and Elizabethkingia meningoseptica.

ENTEROBACTERALES DATABASE
* Added 27 new species: Rahnella bruchi, R. inusitata, R. variigena, R. victoriana, R. woolbedingensis, Serratia symbiotica, S. quinivorans, S. aquatilis, S. bockelmannii, S. nevei, S. oryzae, S. inhibens, S. microhaemolytica, Chimaeribacter arupi, C. coloradensis, C. californicus, Yersinia alsatica, Y. artesiana, Y. proxima, Y. vastinensis, Y. thracica, Y. rochesterensis, Y. nurmii, Y. entomophaga, Y. hibernica, Y. canariae and Y. enterocolitica subsp. palearctica.

LACTOBACILLUS DATABASE
* Moving Lactobacillus bombintestini, Lactobacillus garii and Lactobacillus suantsaiihabitans to genera Apilactobacillus, Lactiplantibacillus and Levilactobacillus, respectively.
* Added 9 new species isolated from bees and plants: Apilactobacillus apisilvae, Bombilactobacillus apium, B. thymidiniphilus, B. folatiphilus, Nicolia spurrieriana, Fructilactobacillus cliffordii, F. hinvesii, F. carniphilus and F. myrtifloralis.
* Emended description of several other species.

BIFIDOBACTERIUM DATABASE
* Added 5 new species isolated from water kefir and feces of tamarin, gorilla and bats: Bifidobacterium moukalabense, B.rousetti, B. tibiigranuli, B. vespertilionis and B. vansinderenii.

GENERAL
* New feature in "Analitics" section: the "Strongest test" option can list the best tests to differentiate a species from other taxa inside their genus or family.
* Kauffmann-White classification:
- Added three Salmonella serovars: two subsp. salamae and one subsp. houtenae.
- Salmonella serovars combinations and synonyms that have been withdrawn from the Kauffman-White scheme were added as historical reference.

ENTEROBACTERIA DATABASE
* Added 33 species isolated from plants and nematodes. Some of them are pathogens for onion, pear, apple, pineapple, eucalyptus, and maize. New entries: Pantoea allii, P. brenneri, P. eucrina, P. septica, P. conspicua, P. anthophila, P. deleyi, P. eucalypti, P. vagans, P. coffeiphila, P. rodasii, P. rwandensis, P. wallisii, Erwinia oleae, E. gerundensis, E. piriflorinigrans, E. uzenensis, E. endophytica, E. teleogrylli, Mixta calida, M. gaviniae, M. theicola, M. tenebrionis, Tatumella saanichensis, Xenorhabdus budapestensis, X. ehlersii, X. innexi, X. indica, X. szentirmaii, X. eapokensi, X. thuongxuanensis, X. ishibashii, and X. khoisanae.
* Emended description of some members of the genera Xenorhabdus and Pantoea.
* "Mucate fermentation" test was abandoned due to the low value for identification.
* Correction of "esculin hydrolysis" test for Pantoea stewartii subsp. stewartii.
* Due to division of the order Enterobacterales into the 5 families: Enterobacteriaceae, Erwiniaceae, Pectobacteriaceae, Yersiniaceae, Hafniaceae, Morganellaceae, and Budviciaceae, the "Enterobacteriaceae" database was renamed "Enterobacterales" and this is how it will appear on all ABIS pages.

BIFIDOBACTERIUM DATABASE
* Added 9 species isolated from human, monkeys, sloth and dog feces: Bifidobacterium canis, B. catulorum, B. choloepi, B. faecale, B. primatium, B. scaligerum, B. felsineum, B. simiarum and B.jacchi.
* Emended description of several bifidobacteria.

ENTEROBACTERIA DATABASE
* Added 11 new species from genera Photorhabdus, Proteus and Providencia: Photorhabdus cinerea, P. heterorhabditis, P. stackebrandtii, P. noenieputensis, P. namnaonensis, Proteus alimentorum, P. terrae, P. columbae, P. cibi, P. faecis and Providencia thailandensis.
* Emended description of Proteus hauseri and Proteus penneri the former P. vulgaris biogroups 3 and 1, respectively.

LACTOBACILLUS DATABASE
* Added 2 new species: Lentilactobacillus laojiaonis and Furfurilactobacillus milii.
* Emended description of Lentilactobacillus parabuchneri, Lentilactobacillus rapi and Lentilactobacillus senioris.

BIFIDOBACTERIUM DATABASE
* Added 30 new species: Bifidobacterium thermacidophilum, B. porcinum, B. tsurumiense, B. aerophilum, B. avesanii, B. ramosum, B. aesculapii, B. anseris, B. criceti, B. italicum, B. imperatoris, B. margollesii, B. parmae, B. hapali, B. tissieri, B. myosotis, B. apousia, B. choladohabitans, B. polysaccharolyticum, B. coryneforme, B. apri, B. aquikefiri, B. crudilactis, B. psychraerophilum, B. castoris, B. callimiconis, B. goeldii, B. samirii, B. dolichotidis and B. callitrichidarum.
* Emended description of several other Bifidobacteria.

ENTEROBACTERIA DATABASE
* Added 4 bioluminescent bacteria: Photorhabdus laumondii subsp.clarkei, P. bodei, P. kayaii and P. thracensis.

PSEUDOMONAS DATABASE
* Added 13 soil bacteria some of them involved in nitrogen fixation: Formosimonas limnophila, Hydromonas duriensis, Lautropia mirabilis, Limnobacter litoralis, Limnobacter thiooxidans, Mycoavidus cysteinexigens, Quisquiliibacterium transsilvanicum, Azotobacter chroococcum, A. beijerinckii, A. bryophylli, A. nigricans, A. vinelandii, A. armeniacus and Azorhizophilus paspali.

BIFIDOBACTERIUM DATABASE
* Added 22 bifidobacteria: Bifidobacterium bombi, B. erythrocebi, B. moraviense B. oedipodis B. olomucense, B. panos, B. eulemuris, B. stellenboschense, B. scardovii, B. lemurum, B. saguini, B. biavatii, B. callitrichos, B. reuteri, B. gallinarum subsp. gallinarum, B. gallinarum subsp. saeculare, B. pullorum, B. gallicum, B. magnum, B. merycicum, B. mongoliense, and B. ruminantium.

STREPTOCOCCUS DATABASE
* Added 24 new species from genera Enterobacter and Vagococcus, isolated from: the gut of wax moth (Galleria mellonella), cloaca of common kingfishers (Alcedo atthis), camel milk, faeces of timber rattlesnake (Crotalus horridus), gut of the camel cricket (Diestrammena coreana), chinese pickle juice, swine manure, cotton flower (Gossypium hirsutum L.), Bitto cheese, green-olive fermentations, Aegopodium flowers, water, chicken meat, faeces of yaks (Bos grunniens), fermented cow milk, ground beef, foot wound, grain used to make silage, small intestine of a marten (Martes flavigula) and cloacal content of a snow finch (Montifringilla taczanowskii): Enterobacter innesii, E. alcedinis, E. bulliens, E. crotali, E. diestrammenae, E. dongliensis, E. hulanensis, E. nangangensis, E. pingfangensis, E. songbeiensis, E. eurekensis, E. lemanii, E. florum, E. lactis, E. olivae, E. plantarum, E. quebecensis, E. ureasiticus, E. rivorum, E. rotai, E. ureilyticus, E. saigonensis, E. xiangfangensis, E. wangshanyuanii, Vagococcus teuberi, V. bubulae, V. vulneris, V. silagei, V. martis, and V. xieshaowenii.
* Emended description of Vagococcus penaei.
* Lancefield antigens database updated.

ENTEROBACTERIA DATABASE
* Emended description of Dickeya chrysanthemi, D. paradisiaca, Pectobacterium carotovorum, P. cacticida, and P. wasabiae.
* Transfer of Brenneria quercina (Hildebrand and Schroth 1967) Hauben et al. 1999. to genus Lonsdalea as Lonsdalea quercina (Hildebrand and Schroth 1967) Brady et al. 2012 and emended description.
* Transfer of Dickeya paradisiaca (Fernandez-Borrero and Lopez-Duque 1970) Samson et al. 2005 to genus Musicola as Musicola paradisiaca (Fernandez-Borrero and Lopez-Duque 1970) Hugouvieux-Cotte-Pattat et al. 2021.
* Added 21 species, most of them plant pathogens:
-Lonsdalea britannica, L. iberica, L. populi;
-Brenneria populi, B. roseae subsp. roseae, B. roseae subsp. americana, B. goodwinii, B. corticis;
-Dickeya chrysanthemi biovar parthenii, D. dianthicola, D. dadantii subsp. dieffenbachiae, D. aquatica, D. fangzhongdai;
-Pectobacterium carnegiana, P. odoriferum, P. brasiliense, P. polaris, P. actinidiae, and P. polonicum.
-Sodalis glossinidius, S. praecaptivus.

LACTOBACILLUS DATABASE
* Added 7 more species isolated from woody biomass, human vaginal discharge, mushroom substrate, pressed yeast, milk, cheese, fermenting plant material, grain silage, leaves of a strawberry plant, corn steep liquor, and sugar-vegetable fermented beverage: Lactobacillus jensenii, Lentilactobacillus fungorum, Lentilactobacillus buchneri, Latilactobacillus fragifolii, Lacticaseibacillus zeae, and Lentilactobacillus kosonis.
* Emended description of Lentilactobacillus rapi, L. otakiensis, L. diolivorans, L. farraginis, L. kisonensis, L. parabuchneri, and L. sunkii.

OTHERS
The ABIS online application has returned to the Google Play Store.
It can be installed on android mobile devices from https://play.google.com/store/apps/details?id=net.tgw1916

BIFIDOBACTERIUM GRAND OPENING
Opening of the Bifidobacterium identification for public us starting with a 29 species database.

ENTEROBACTERACEAE DATABASE
Added 56 species belonging to genus Enterobacter and other 18 new genera: Kalamiella, Phaseolibacter, Pseudocitrobacter, Biostraticola, Cronobacter, Siccibacter, Franconibacter, Gibbsiella, Saccharobacter, Budvicia, Hafnia, Chania, Phytobacter, Izhakiella, Kosakonia, Mangrovibacter, Rosenbergiella, and Scandinavium.

LACTOBACILLUS DATABASE
Added 4 new species: Limosilactobacillus urinaemulieris, Limosilactobacillus portuensis, Secundilactobacillus folii and Lactobacillus nasalidis.

VARIA
Emended description of Erysipelothrix rhusiopathiae, Erysipelothrix tonsillarum, and Citricoccus muralis. Correction of misspelled name Yersinia frederiksenii.

ANNOUNCEMENT
From November we will start working on the Bifidobacterium database, which we hope will be accessible to the public in a few months.

GENERAL FIXES
* In the "Results" page the similarity percent is no longer displayed by the name of the taxon but next to probability percent, in order to help the user better decide on the identification.
The printout of the results has been optimized for grayscale printers.
* Fixed an error causing some elements on the "Input" page to overlap when using the Mozilla Firefox browser.
Radio buttons were returned to square shape.

DATABASE CHANGES
* Enterobacteriaceae database extended by 4 new species isolated from microbial fuel cell, fresh water lake and beetles: Raoultella electrica, Limnobaculum parvum, Intestinirhabdus alba and Enterobacillus tribolii.
* Staphylococcus/Micrococcus database extended by 24 species isolated from human oral cavity, carious dentine, and saline soils: Rothia dentocariosa, R. aeria, R. mucilaginosa, R. nasimurium, R. amarae, R.terrae, R. endophytica, R. aerolata, Yaniella halotolerans, Y. flava, Y. soli, Enteractinococcus fodinae, Tersicoccus phoenicis, T. solisilvae, Nesterenkonia halobia, N. halotolerans, N. halophila, N. aurantiaca, N. lutea, N. sandarakina, N. jeotgali, N. massiliensis, N. endophytica, and N. natronophila.
* Corynebacterium/Actinomyces database extended by 39 species isolated from diseased salmonids, saline soils, water, penguin guano, air, human ear, porcupine faeces, fermented seafood, and deep-sea sediments: Renibacterium salmoninarum, Arthrobacter rhombi, A. roseus, A. nanjingensis, A. humicola, A. oryzae, A. pokkalii, A. silvisoli, A. celericrescens, A. psychrochitiniphilus, A. russicus, Auritidibacter ignavus, Glutamicibacter halophytocola, Paeniglutamicibacter psychrophenolicus, Pseudarthrobacter niigatensis, P. phenanthrenivorans, Micrococcoides hystricis, Garicola koreensis, Zhihengliuella halotolerans, Z. alba, Z. salsuginis, Z. flava, Nesterenkonia aethiopica, N. suensis, N. flava, N. alba, N. lacusekhoensis, N. alkaliphila, N. populi, N. muleiensis, N. pannonica, N. salmonea, N. sphaerica, Psychromicrobium lacuslunae, P. silvestre, Specibacter cremeus, Glutamicibacter soli, G. endophyticus, and Sinomonas halotolerans.
* Emended description of Geobacillus stearothermophilus, Pragia fontium, Arthrobacter woluwensis, Arthrobacter globiformis, Lactobacillus brevis, Lactobacillus casei and Lactobacillus curvatus.

* New Enterobacteriaceae isolated from human feces, urine, tomato, and well water: Escherichia ruysiae, Klebsiella huaxiensis, K. indica, Citrobacter cronae, C. europaeus, C. pasteurii, C. portucalensis, and C. telavivensis.
The new genus Lelliottia includes the former Enterobacter amnigenus and Enterobacter nimipressuralis, and a new species Lelliottia jeotgali isolated from Korean fermented clam.
* New Micrococcaceae species isolated from cryoconite, poultry litter, pine caterpillar, soil, water, and marine sediments: Arthrobacter cryoconiti, A. stackebrandtii, A. psychrolactophilus, A. cupressi, A. deserti, A. pityocampae, A. agilis, A. subterraneus, A. halodurans, A. livingstonensis, A. paludis, Paeniglutamicibacter cryotolerans, and Pseudarthrobacter enclensis.
* New Lactobacillus species isolated from Marmota hymalayana, human urine and vinegar: Lactobacillus xujianguonis, Lactobacillus mulieris, and Acetilactobacillus jinshanensis, respectively.
* Emended description of Micrococcus (Arthrobacter) agilis, Bacillus thuringiensis, Lactobacillus rossiae and Fructilactobacilus fructivorans.
Enterobacter aerogenes has been moved to genus Klebsiella as Klebsiella aerogenes.
Enterobacter cloacae and Enterobacter dissolvens were renamed as E. cloacae subsp. cloacae and E. cloacae subsp. dissolvens, respectively.
Enterobacter gergoviae has been moved to genus Pluralibacter as Pluralibacter gergoviae.

TAXONOMY CHANGES
* Genus Lactobacillus has been divided in 23 novel genera (Zheng et al. 2020): Holzapfelia, Amylolactobacillus, Bombilactobacillus, Companilactobacillus, Lapidilactobacillus, Agrilactobacillus, Schleiferilactobacillus, Loigolactobacilus, Lacticaseibacillus, Latilactobacillus, Dellaglioa, Liquorilactobacillus, Ligilactobacillus, Lactiplantibacillus, Furfurilactobacillus, Paucilactobacillus, Limosilactobacillus, Fructilactobacillus, Acetilactobacillus, Apilactobacillus, Levilactobacillus, Secundilactobacillus and Lentilactobacillus.
* L. reuteri has been splitted into 6 subspecies (Li et al. 2021): Limosilactobacillus reuteri subsp. kinnaridis, Limosilactobacillus reuteri subsp. murium, Limosilactobacillus reuteri subsp. porcinus, Limosilactobacillus reuteri subsp. reuteri, and Limosilactobacillus reuteri subsp. rodentium.
* Order 'Enterobacteriales' has been replaced by the new order Enterobacterales (Adeolu et al. 2016) and divided into the families Enterobacteriaceae, Erwiniaceae, Pectobacteriaceae, Yersiniaceae, Hafniaceae, Morganellaceae, and Budviciaceae.

SYSTEM CHANGES
* "Spore production" discrimination test has been replaced by "Rod-shaped cell" test due to introduction of genus Pediococcus (lactic acid cocci) in Lactobacillus database.

DATABASE CHANGES
* Enterobacteria database Emended description of Escherichia albertii.
Lactobacillus database
* 31 species isolated from soil, fermented foods, grape must, beer, cow manure, geese faeces, bees and rodents intestines, were added: Companilactobacillus suantsaicola, C. bobalius, Lactobacillus suantsaiihabitans, L. bombintestini, L. selangorensis, L. amylolyticus, Liquorilactobacillus uvarum, Limosilactobacillus balticus, L. albertensis, L. rudii, L. agrestis, L. fastidiosus, Levilactobacillus cerevisiae, L. enshiensis, L. suantsaii, Lentilactobacillus curieae, Lacticaseibacillus chiayiensis, L. brantae, Furfurilactobacillus curtus, Lapidilactobacillus (Pediococcus) dextrinicus, Pediococcus acidilactici, P. pentosaceus, P. damnosus, P. parvulus, P. inopinatus, P. claussenii, P. cellicola, P. stilesii, P. ethanolidurans, P. siamensis, and P. argentinicus.
Corynebacterium database
* 26 species isolated from air, soil, sea water, bamboo, tobacco leaves, American holly, wall paintings, chicken feces, sewage, and cheeses, were added: Arthrobacter castelli, A. monumenti, A. parietis, A. pigmenti, A. tecti, A. tumbae, A. citreus, A. bambusae, A. ramosus, A. pascens, A. koreensis, Glutamicibacter mysorens, G. nicotianae, G. arilaitensis, G. bergerei, Pseudarthrobacter defluvii, P. chlorophenolicus, P. polychromogenes, P. oxydans, Paeniglutamicibacter gangotriensis, P. kerguelensis, P. sulfureus, Paenarthrobacter nicotinovorans, P. aurescens, P. nitroguajacolicus, and P. ilicis.

* 21 new Lactobacillus species isolated from traditional fermented foods and beverage, pickles, Ceddar cheese, bee bread, swine intestine, wild mice intestine, birds intestine, human gingival crevices, saliva, faeces, and spent mushroom substrates have been added: Lactobacillus metriopterae, L. hulanensis, L. porci, L. porcinae, L. panisapium, L. rodentium, L. shenzhenensis, L. uli, L. rimae, L. minutus, L. mixtipabuli, L. plajomi, L. modestisalitolerans, L. sicerae, L. wasatchensis, L. parabrevis, L. paragasseri, L. colini, L. silagincola, L. pentosiphilus, and L. salitolerans.
* Emended description of L. curvatus, L. similis, L. paracollinoides, L. acidipiscis, L. pobuzihii, L. satsumensis, L. lindneri, L. taiwanensis, and L. collinoides.
* 12 soil bacteria from genera Arthrobacter, Sinomonas and Paeniglutamicibacter have been added: Arthrobacter globiformis, A. alkaliphilus, A. alpinus, Paeniglutamicibacter antarcticus, Sinomonas albida, S. echigonensis, S. humi, S. atrocyanea, S. soli, S. flava, S. mesophila, and S. susongensis.
* 18 Mycobacterium species have been added: M. paraseoulense, M. kyorinense, M. lehmannii, M. neumannii, M. litorale, M. llatzerense, M. mantenii, M. neglectum, M. palauense, M. noviomagense, M. oryzae, M. paraffinicum, M. paragordonae, M. riyadhense, M. shinjukuense, M. stephanolepidis, M. virginiense, and M. vulneris.
* Emended description of several Mycobacterium species.
* Mycobacterium database update is complete and is running at full capacity.
Other changes:
* A SSL certificate bug that trigger the antivirus' security warning or browser's page blocking as "untrusted" has been removed.
* Suggestion for a minimum number of tests has been introduced at the bottom of the "Input tests" page.

HELICOBACTER
* Added Helicobacter mastomyrinus to identification database.

PASTEURELLACEAE
* Added Gallibacterium group V, Gallibacterium genomospecies 1, 2 and 3.
* Minor adjustments of Gallibacterium melopsittaci biochemical matrix.

CLOSTRIDIUM
* Correction of Clostridium tetani's gelatin hydrolysis test.

MYCOBACTERIUM
* Emended description of Mycobacterium farcinogenes, M. senegalense, M. gilvum, M. obuense, M. chubuense, M. houstonense, M. aubagnense, M. poriferae, and M. psychrotolerans.
* 38 species added: M. madagascariense, M. hodleri, M. murale, M. monacense, M. novocastrense, M. goodii, M. wolinskyi, M. elephantis, M. poriferae, M. psychrotolerans, M. alsense, M. angelicum, M. aquiterrae, M. arcueilense, M. lutetiense, M. montmartrense, M. celeriflavum, M. decipiens, M. eburneum, M. paraense, M. europaeum, M. stomatepiae, M. sherrisii, M. fragae, M. grossiae, M. helvum, M. sarraceniae, M. hippocampi, M. icosiumassiliensis, M. insubricum, M. iranicum, M. komaniense, M. malmesburyense, M. koreense, M. parakoreense, M. kyogaense, M. chlorophenolicum, and M. pyrenivorans.

LACTOBACILLUS
* Emended description of Lactobacillus gigeriorum, L. taiwanensis, L. johnsonii, L. gasseri, L. futsaii, L. farciminis, L. heilongjangensis, L. musae, L. nantensis, L. sanfranciscensis, and L. lindneri.
* 23 new species added: L. herbarum, L. hominis, L. pasteurii, L. huachuanensis, L. crustorum, L. nuruki, L. musae, L. zhachilii, L. kimchiensis, L. vespulae, L. ixorae, L. yonginensis, L. pingfangensis, L. daoliensis, L. nangangensis, L. daowaiensis, L. dongliensis, L. songbeiensis, L. kaifaensis, L. mudanjiangensis, L. songhuajiangensis, L. nenjiangensis, and L. oligofermentans.

ENTEROBACTERIA
* New Escherichia species added in Enterobacteriaceae database: Escherichia marmotae isolated from faeces of Marmota himalayana.

MYCOBACTERIA
* Mycobacterium database extended by 10 species: M. holsaticum, M. pulveris, M. conceptionense, M. canariasense, M. mageritense, M. confluentis, M. mucogenicum, M. aubagnense, M. phocaicum, and M. salmoniphilum.

LACTOBACILLI
* Emended biochemical description of Lactobacillus perolens.
* Lactobacillus plantarum subsp. argentoratensis was elevated to the species level as Lactobacillus argentoratensis.
* 30 new Lactobacillus species isolated from the intestinal tract of a bees, flowers, plants, fermented beverages, fermented foods, spoiled soft drinks, cocoa bean heap fermentation, cow udder, oral cavity of Guinea pig, faeces of a gorilla, a jackal (Canis mesomelas) and racoons (Procyron lotor) were added to Lactobacillus database: L. hegangensis, L. suibinensis, L. daqingensis, L. yichunensis, L. mulanensis, L. achengensis, L. wuchangensis, L. gannanensis, L. binensis, L. angrenensis, L. zhaodongensis, L. argentoratensis, L. micheneri, L. timberlakei, L. quenuiae, L. bombicola, L. camelliae, L. thailandensis, L. cacaonum, L. fabifermentans, L. caviae, L. faecis, L. formosensis, L. futsaii, L. garii, L. gorillae, L. halodurans, L. salsicarnum, L. harbinensis, and L. heilongjiangensis.

STREPTOCOCCI
* Reclassification of Streptococcus dentisani as Streptococcus oralis subsp. dentisani, Streptococcus tigurinus as Streptococcus oralis subsp. tigurinus, and Streptococcus oligofermentans as a later synonym of Streptococcus cristatus.
* Minor biochemical adjustments of Streptococcus mitis, S. sanguinis, S. gordonii, S. gallolyticus subspecies gallolyticus, macedonicus and pasteurianus.

CAMPYLOBACTER
* Campylobacter sputorum species was splited into three biovars: sputorum, paraureolyticus and fecalis.
* Minor biochemical adjustments of several Arcobacter and Campylobacter species.

OTHERS
* New functions in 'Server status' page: 30 days connections and server detection.

LACTOBACILLI
* Splitting arabinose and xylose fermentation tests to their L- and D- (laevus and dexter) isomers.
* Adding glycogen, turanose and N-acetyl-D-glucosamine fermentation tests.
* Separation of Lactobacillus pentosus and L. paraplantarum from L. plantarum identification matrix and minor biochemical adjustments.
* Emmended description of several Lactobacillus species: Lactobacillus curvatus subsp. melibiosus, L. cypricasei, L. vaccinostercus (L. durianis), L. murinus, L. oryzae, L. pentosus, and L. paraplantarum.
* 28 new Lactobacillus species added: Lactobacillus silagei, L. hokkaidonensis, L. oryzae, L. kimchicus, L. baiquanensis, L. bayanensis, L. keshanensis, L. kedongensis, L. jidongensis, L. yilanensis, L. hulinensis, L. mishanensis, L. zhongbaensis, L. bambusae, L. paraplantarum, L. baoqingensis, L. jixianensis, L. jiayinensis, L. zhaoyuanensis, L. lindianensis, L. kimchii, L. delbrueckii subsp. sunkii, L. huananensis, L. tangyuanensis, L. fuyuanensis, L. tongjiangensis, L. fujinensis, and L. mulengensis.

PASTEURELLACEAE
* New Gallibacterium species were added to Pasteurellaceae database: Gallibacterium melopsittaci and G. trehalosifermentans.
* Gallibacterium anatis was splitted in two biovars "anatis" and "haemolytica".
* Minor adjustments of biochemical matrices of G. anatis biovar anatis and G. salpingitidis.

MYCOBACTERIA
* Mycobacterium database extended by 33 species: Mycobacterium vaccae, M. gilvum, M. obuense, M. chubuense, M. rhodesiae, M. aichiense, M. tokaiense, M. agri, M. fallax, M. brumae, M. aurum, M. neoaurum, M. thermoresistibile, M. diernhoferi, M. gadium, M. duvalii, M. komossense, M. austroafricanum, M. vanbaalenii, M. sphagni, M. immunogenum, M. alvei, M. frederiksbergense, M. doricum, M. ostraviense (M. kansasii subtype IV), M. kubicae, M. houstonense, M. boenickei, M. neworleansense, M. brisbanense, M. porcinum, M. septicum, and M. moriokaense.

OTHERS
* Correction in Analytics page when using two taxa comparison function.

1. An experimental Mycobacterium identification database is now open for public use.
2. Kauffman-White scheme: 74 new Salmonella serovars have been added and 25 previously described serovars have been updated.
3. Correction of Salmonella Poona antigenic formula and correction of Salmonella Kaevlinge and Widemarsch names.
4. Ten Kocuria species were added to Staphylococcus database: Kocuria turfanensis, K. atrinae, K. dechangensis, K. gwangalliensis, K. halotolerans, K. salsicia, K. pelophila, K. tytonis, K. uropygialis, and K. uropygioeca.
5. Fifteen new Lactobacillus species were added to Lactobacillus database: Lactobacillus apinorum, L. apis, L. mellifer, L. mellis, L. melliventris, L. kimbladii, L. helsingborgensis, L. kullabergensis, L. bombi, L. tucceti, L. allii, L. insicii, L. furfuricola, L. backi, and L. iwatensis.
6. Minor adjustments of biochemical matrices of several Lactobacillus species.
7. Adjustment of some biochemical characters of Vibrio metschnikovii.
8. Site migration from http:// to https://
9. ABIS Android application is no longer listed in Google Play Store. It can only be dowloaded as independent app from Regnum Prokaryotae site or ABIS online.

1. New functions in "Analitics" section:
-comparison of two taxa by morphological and biochemical characters.
-listing all taxa in the database that behave the same for a specified biochemical value.
2. Dark theme aplied to ABIS (requires to clear browser's cache to be effective).
3. Streptococcus cristatus inclusion in Lancefield group F and emmended biochemical description.
4. Based on 16S rRNA gene phylogenetic analysis, Burkholderia (Pseudomonas) andropogonis has been moved to genus Robbsia as Robbsia andropogonis.
5.Staphylococcus database-The "fibrinolysin" test was removed from input panel and replaced with esculin hydrolysis and gelatin hydrolysis as they have a higher significance for identification.
-"Carotenoid pigment production" test was renamed “Yellow-, orange-, pink- or red-pigmented colonies” in order to adapt Staphylococcus database for Micrococcus and Kocuria identification
-30 species were added: Aliicoccus persicus, Auricoccus indicus, Corticicoccus populi, Micrococcus luteus, M. lylae, M. antarcticus, M. endophyticus, M. flavus, M. terreus, M. yunnanensis, M. cohnii, M. aloeverae, Micrococcus (Arthrobacter) agilis, Neomicrococcus aestuarii, N. lactis, Citricoccus alkalitolerans, C. muralis, C. parietis, C. zhacaiensis, Kocuria rosea, K. kristinae, K. varians, K. aegyptia, K. palustris, K. carniphila, K. himachalensis, K. polaris, K.marina, K. rhizophila, and Flaviflexus salsibiostraticola.6. Corynebacterium database- 9 species added: Actinotignum sanguinis, A. schaalii, A. urinale, Flaviflexus huanghaiensis, F. salsibiostraticola, Mobiluncus curtisii, M. mulieris, Varibaculum anthropi, and V. cambriense.
Note: Flaviflexus salsibiostraticola was included both in Staphylococcus and Corynebacterium identification databases due to the Gram-positive cocci morphology and the affiliation to the class Actinobacteria by 16S rRNA gene sequence.

GENERAL FIXES
Correction of Gemella haemolysans, Gemella sanguinis, Gemella bergeri and Gemella cuniculi haemolysis character.
Added [Klebsiella] extension to members of the genus Raoultella in order to emphasise their basonym.
Graphical issue of antibiogram result printing button was solved.
Clostridium difficile and Clostridium mangenotii were moved to Family Peptostreptococcaceae, Genus Clostridioides as Clostridioides difficile and Clostridioides mangenotii, respectively.
A Server Status menu is available. The user is informed about time left before session expires. The average lifetime of stored information in the ABIS server is 24 minutes. If the session expires, there is a risk that the browser may lose those information, and the expected results may be inaccurate. In this case restarting the program is required.
Other features: information about active connections (live users), last database update, number of species, subspecies and serovars in db, and a limited per session activity log.

DATABASES CHANGES
34 new species were added in Non-fermenters (Pseudomonas) and Corynebacterium databases: Actinomyces oricola, A. radicidentis, A. ruminicola, A. slackii, A. suimastitidis, A. timonensis, A. urogenitalis, A. vaccimaxillae, A. vulturis, A. weissii, Actinobaculum suis, A. massiliense, Cupriavidus necator, C. basilensis, C. campinensis, C. metallidurans, C. gilardii, C. pauculus, C. respiraculi, C. laharis, C. pinatubonensis, C. alkaliphilus,C. nantongensis, C. yeoncheonensis, C. numazuensis, C. oxalaticus, C. pampae, C. taiwanensis, Chitinimonas koreensis, C. lacunae, C. naiadis, C. prasina, C. taiwanensis and C. viridis.

* "Growing on MacConkey agar" character of Pasteurella multocida subspecies has been changed from "variable" to "usually negative".
* Minor biochemical adjustments on Pasteurella multocida subsp. multocida and gallinarum, Streptococcus suis, Granulicatella adiacens, Streptococcus downei, Streptococcus intermedius, Burkholderia plantarii, Caballeronia glathei, Caballeronia sordidicola, Caballeronia zhejiangensis, Paraburkholderia bannensis, Paraburkholderia acidipaludis, Paraburkholderia caledonica and Paraburkholderia fungorum.
* 48 species added to Non-fermenters and Corynebacterium databases: Paraburkholderia endofungorum, P. ferrariae, P. ginsengisoli, P. heleia, P. hospita, P. terricola, P. humisilvae, P. solisilvae, P. rhizosphaerae, P. insulsa, P. jirisanensis, P. kururiensis, P. metalliresistens, P. mimosarum, P. monticola, P. nodosa, P. oxyphila, P. pallidirosea, P. phenoliruptrix, P. phymatum, P. phytofirmans, P. piptadeniae, P. rhizoxinica, P. ribeironis, P. rhynchosiae, P. sabiae, P. sacchari, P. sartisoli, P. silvatlantica, P. soli, P. sprentiae, P. susongensis, P. symbiotica, P. terrae, P. tropica, P. tuberum, P. unamae, P. xenovorans, Actinomyces odontolyticus, A. viscosus, A. dentalis, A. denticolens, A. haliotis, A. hongkongensis, A. howellii, A. nasicola, A. naturae and Cellulomonas humilata (Actinomyces humiferus).

* The minimum number of input tests has been increased from 8 to 9 when using 'Auto' and 'Normal' modes. The limit of 'Forced' mode has not been changed. ABIS may require more tests if the identification results are not reliable.
* Non-fermenters and Corynebacterium databases - 47 species from Burkholderia, Pandoraea, Caballeronia, Paraburkholderia and Actinomyces genera have been added: Burkholderia contaminans, B. lata, B. humptydooensis, B. pseudomultivorans, B. puraquae, B. singularis, B. stagnalis, B. territorii, B. thailandensis, B. ubonensis, Caballeronia arvi, C. choica, C. humi, C. telluris, C. terrestris, C. udeis, C. cordobensis, C. grimmiae, C. jiangsuensis, C. megalochromosomata, C. sordidicola, C. zhejiangensis, Pandoraea norimbergensis, P. pulmonicola, P. apista, P. pnomenusa, P. sputorum, Paraburkholderia aspalathi, P. azotifigens, P. bannensis, P. bryophila, P. megapolitana, P. caffeinilytica, P. sediminicola, P. caledonica, P. fungorum, P. caribensis, P. diazotrophica, P. dilworthii, P. dipogonis, P. eburnea, Actinomyces bovis, A. georgiae, A. gerencseriae, A. israelii, A. johnsonii, and A. oris.

DATABASES CHANGES
* Minor biochemical adjustments were made for Burkholderia cepacia (genomovar I), Burkholderia gladioli (Pseudomonas antimicrobica), and Ralstonia pickettii.
* Alkaline phosphatase test values for several Pseudomonas, Burkholderia and Oligella species were revised.
* 34 new species were added in Non-fermenters (Pseudomonas) and Corynebacterium databases: Burkholderia multivorans (B. cepacia genomovar II), B. cenocepacia (B. cepacia genomovar III), B. stabilis (B. cepacia genomovar IV), B. vitenamiensis (B. cepacia genomovar V), B. dolosa (B. cepacia genomovar VI), B. ambifaria (B. cepacia genomovar VII), B. anthina (B. cepacia genomovar VIII), B. pyrrocinia (B. cepacia genomovar IX), B. alpina, B. arboris, B. metallica, B. diffusa, B. latens, B. seminalis, Paraburkholderia acidipaludis, P. hiiakae, P. metrosideri, P. paradisi, P. peleae, Caballeronia arationis, C. hypogeia, C. ptereochthonis, C. glebae, C. pedi, C. fortuita, C. calidae, C. concitans, C. peredens, C. temeraria, C. turbans, C. catudaia, Actinomyces massiliensis, A. meyeri, and A. naeslundii.

* Correction of Simmons citrate utilization for Yokenella regensburgei (Koserella trabulsii).
* Added itaconate utilization test in Non-fermenters (Pseudomonas) identification matrix.
* 28 species added in Non-fermenters database: Achromobacter animicus, A. mucicolens, A. insolitus, A. spanius, A. marplatensis, Alcaligenes aquatilis, Alcaligenes endophyticus, Advenella alkanexedens, A. faeciporci, A. incenata, A. kashmirensis subsp. kashmirensis, A. kashmirensis subsp. methylica, A. mimigardefordensis, Azohydromonas australica, Ampullimonas aquatilis, Brackiella oedipodis, Caenimicrobium hargitense, Candidimonas bauzanensis, C. humi, C. nitroreducens, Castellaniella caeni, C. daejeonensis, C. denitrificans, C. ginsengisoli, C. hirudinis, Derxia gummosa, Derxia lacustris and Verticiella sediminum.
* 6 species added in Corynebacterium database: Actinomyces funkei, A. graevenitzii, A. hominis, A. hordeovulneris, A. hyovaginalis and A. liubingyangii.

- Added urease test in Streptococcus identification scheme.
- Added hemolysis character in Vibrio and Aeromonas identification scheme, while phenylalanine deaminase test was removed.
- Added Streptococcus phocae subsp. salmonis in Lancefield group G.
- A script bug showing in duplicate the target's similarity was removed.
- Biochemical characters adjustments were made for some taxa from Bacillus, Pasteurella, Streptococcus and Vibrio databases.
- 27 more species were added:
*Non-fermenters database: Alcaligenes faecalis, Castellaniella defragrans, Azohydromonas lata, Achromobacter xylosoxidans, Achromobacter denitrificans, Achromobacter ruhlandii, Achromobacter piechaudii, Achromobacter aegrifaciens, Achromobacter insuavis, Achromobacter anxifer, Achromobacter dolens, Achromobacter agilis, Achromobacter pestifer, Achromobacter deley and Achromobacter kerstersii.
*Streptococcus database: Lactococcus fujiensis, Streptococcus parasuis and Enterococcus saccharolyticus subsp. taiwanensis.
*Pasteurella database: Pasteurella skyensis.
*Vibrio database: Aliivibrio finisterrensis, Aliivibrio sifiae.
*Corynebacterium database: Actinomyces bowdenii, Actinomyces canis, Actinomyces cardiffensis, Actinomyces catuli, Actinomyces coleocanis, and Actinomyces europeus.

Taxonomic changes:
- Glaeser et al. 2013 proposed the transfer of Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus.
- Actinobacillus muris was moved in genus Muribacter as Muribacter muris.
- The former Pasteurella species B, Mutters et al. 1985 is now Pasteurella oralis Christensen et al. 2012.

Changes in Results page:
-"Matching" indicator has been renamed "Similarity" - which shows the closeness between input tests and biochemical patterns in database.
-"Accuracy" indicator has been replaced by "Matrix integrity" - which reflects the gaps in database.
-"Probability", a probabilistic indicator based on Bayes' theorem (Lapage et al. 1973) has been introduced.
Pseudomonas identification scheme has been extended by 19 substrate utilization tests: acetate, adipate, L-alanine, arabitol, caprate, erythritol, gluconate, 5-ketogluconate, glycerol, L-histidine, lactate, L-leucine, malate, malonate , maltose, N-acetylglucosamine, phenylacetate, propionate and L-serine.
Other changes:
-Diffusible and Non-diffusible non-fluorescent pigments were combined in a single query: Nonfluorescent pigment.
-Corynebacterium identification scheme has been extended by 4 tests: alpha-galactosidase, pyrrolidonyl arylamidase, trypsin and Voges-Proskauer.
-Biochemical adjustments in Staphylococcus, Corynebacterium and Non-fermenters (Pseudomonas) databases.
-Pseudomonas lemoignei has been moved to Genus Paucimonas as Paucimonas lemoignei, type and single species of the genus.
-"Corynebacterium pseudodiphtheriticum" species name has been corrected.
-Access to molecular identification has been restricted.
-Correction of letter L index error in ABIS Encyclopedia.
DATABASES CHANGES
Six species belonging to genera Staphylococcus, Jeotgalicoccus, and Salinicoccus were added in Staphylococcus database: Staphylococcus argensis, Jeotgalicoccus aerolatus, Jeotgalicoccus coquinae, Salinicoccus alkaliphilus, Salinicoccus luteus and Salinicoccus salsiraiae.
Eleven species belonging to genera Trueperella, Arcanobacterium. Rhodococcus, Microbacterium and Actinomyces were added in Corynebacterium database: Trueperella bialowiezensis, Trueperella bonasi, Arcanobacterium canis, Arcanobacterium hippocoleae, Arcanobacterium phocisimile, Arcanobacterium pinnipediorum, Rhodococcus hoagii, Arcanobacterium pluranimalium, Microbacterium arborescens, Actinomyces neuii subsp. anitratus, and Actinomyces neuii subsp. neuii.
Sixteen species belonging to genera Comamonas, Pseudomonas and Burkholderia have been added in Pseudomonas database: Comamonas terrigena, Comamonas aquatica (previously known as Comamonas terrigena DNA group 2), Comamonas kerstersii (previously known as Comamonas terrigena DNA group 3), Pseudomonas brenneri, Pseudomonas cannabina (Pseudomonas syringae pv. Cannabina), Pseudomonas cedrina subsp. cedrina, Pseudomonas cedrina subsp. fulgida, Pseudomonas delhiensis, Pseudomonas kilonensis, Pseudomonas mohnii, Pseudomonas moorei, Pseudomonas mucidolens, Pseudomonas reinekei, Pseudomonas putida biovar B, Pseudomonas tremae (Pseudomonas syringae pv. Tremae) and Burkholderia glathei.
Ten species belonging to geneus Vagococcus were added in Streptococcus database: Vagococcus acidifermentans, Vagococcus carniphilus, Vagococcus elongatus, Vagococcus entomophilus, Vagococcus fessus, Vagococcus fluvialis, Vagococcus humatus, Vagococcus lutrae, Vagococcus penaei, and Vagococcus salmoninarum.

* Graphical revamping and new option button in "Results" page. Salmonella, Streptococcus and some related genera can be further characterized by Kauffman-White and Lancefield schemes, respectively.
* Biochemical adjustments in Non-fermenters (Pseudomonas) and Neisseria databases.
* Correction of "Main Menu" graphical issue in Internet Explorer browser.
* Ten species belonging to Family Enterococcaceae have been added in Streptococcus database: Bavariicoccus seileri,Catellicoccus marimammalium, Melissococcus plutonius, Pilibacter termitis, Tetragenococcus halophilus subsp. flandriensis, Tetragenococcus halophilus subsp. halophilus, Tetragenococcus koreensis, Tetragenococcus muriaticus,Tetragenococcus osmophilus and Tetragenococcus solitarius.
* Three species belonging to Family Actinomycetaceae have been added in Corynebacterium database: Trueperella abortisuis, Trueperella bernardiae and Arcanobacterium phocae.
* Seventeen species belonging to Family Chromobacteriaceae have been added in Neisseria database: Aquitalea magnusonii, Aquitalea denitrificans, Aquitalea pelogenes, Gulbenkiania mobilis, Gulbenkiania indica, Jeongeupia naejangsanensis, Jeongeupia chitinilytica, Laribacter hongkongensis, Leeia oryzae, Paludibacterium purpuratum, Paludibacterium paludis, Paludibacterium yongneupense, Pseudogulbenkiania gefcensis, Pseudogulbenkiania subflava, Silvimonas amylolytica, Silvimonas iriomotensis and Silvimonas terrae

Tributyrin hydrolysis test has been removed.
Eight test have been added: citrate utilization, chitin hydrolysis, casein hydrolysis, alkaline phosphatase, arginine dihydrolase, fermentation of: arabinose, mannitol and trehalose.
Minor biochemical adjustments of Bacillus amyloliquefaciens, Neisseria cinerea, N. meningitidis, N. mucosa, N. polysaccharea, N. sicca, N. subflava, Moraxella caviae / M. cuniculi / M. ovis, and Eikenella corrodens.
31 species added: Chromobacterium violaceum, C. alkanivorans, C. aquaticum, C. haemolyticum, C. piscinae, C. pseudoviolaceum, C. rhizoryzae, C. subtsugae, C. vaccinii, Iodobacter fluviatilis, I. arcticus, I. limnosediminis, Chitiniphilus shinanonensis, Vogesella indigofera, V. amnigena, V. alkaliphila, V. facilis, V. fluminis, V. lacus, V. mureinivorans, V. perlucida; Andreprevotia chitinilytica, A. lacus, Chitinilyticum aquatile, C. litopenaei, Chitinibacter alvei, C. fontanus, C. tainanensis, Deefgea chitinilytica, D. rivuli, and Formivibrio citricus.

CORYNEBACTERIUM DATABASE
8 species added: Microbacterium binotii, M. gubbeenense, M. imperiale, M. liquefaciens, M. oxydans, M. resistens, M. sorbitolivorans, and Arcanobacterium haemolyticum.

GENERAL CHANGES
The "Library" buttons have been removed from the Main Menu because they were confused with the database buttons by some users. They were replaced with a magnifying glass icon placed in the upper left corner of database buttons.

- Added 1 more test in Neisseria database: helical shape, required by Aquaspirillum species.
- Added 4 more tests for Listeria identification: maltose, raffinose, turanose and D-xylitol acidification.
- Correction in Listeria input tests: xylose was replaced with D-xylose. Consequently Listeria rocourtiae xylose-variable test changed to D-xylose-positive.- Casein hydrolysis test was removed due to its very low value for Listeria identification.
- Listeria seeligeri CAMP-test was corrected: negative with Rhodococcus equi and positive with Staphylococcus aureus.

55 new species were added:
LACTOBACILLUS DATABASE
Lactobacillus bifermentans, L. graminis, L. hamsteri, L. kefiri, L. hammesii, L. hayakitensis, L. fornicalis, L. iners, L. koreensis, L. lindneri, L. murinus, L. pontis, L. perolens, L. psittaci, L. satsumensis, L. sharpae, L. siliginis, L. spicheri, L. suebicus and L. sanfranciscensis

LISTERIACEAE DATABASE
Erysipelothrix larvae, Listeria aquatica, L. cornellensis, L. grandensis, L. floridensis, L. riparia, L. booriae, L. newyorkensis, L. weihenstephanensis, L. fleischmannii subsp. fleischmannii, L. fleischmannii subsp. coloradonensis, Allobaculum stercoricanis, Bulleidia extructa, Holdemania filiformis, Solobacterium moorei, Amantichitinum ursilacus and Snodgrassella alvi.
NEISSERIACEAE DATABASE
Aquaspirillum bengal, A. arcticum, A. metamorphum, A. polymorphum, A. putridiconchylium, A. sinuosum, A. serpens, Comamonas aquatica (Aquaspirillum aquaticum), Curvibacter delicatus (Aquaspirillum delicatum), Giesbergeria (Aquaspirillum) anulus, Giesbergeria (Aquaspirillum) giesbergeri, Herbaspirillum (Aquaspirillum) autotrophicum, Hylemonella gracilis (Aquaspirillum gracile), Insolitispirillum (Aquaspirillum) peregrinum, Magnetospirillum (Aquaspirillum) magnetotacticum, Microvirgula aerodenitrificans (Aquaspirillum dispar), Novispirillum (Aquaspirillum) itersonii and Simplicispira (Aquaspirillum) psychrophilum.

SOFTWARE CHANGES
- Reorganisation of the Biochemical identification menu on Gram-staining basis;
- "Details" page was removed. The "Results" page is now containing the identified taxa, id%, accuracy, entered tests, opposing tests and suggestions for the differentiation of close taxa;
- Printing results with larger characters;
- Minor change in id% calculating formula in order to allow the identification of rare and poorly described taxa. Note that this taxa are better identified by using "Target" mode;
- Change of accuracy (acc%) calculating formula.
DATABASE CHANGES
- Minor biochemical adjustments to Enterobacter nimipressuralis & Enterobacter pyrinus;
- Minor biochemical adjustments to Bacillus subtilis and its biochemically similar taxa: B. atrophaeus / B. vallismortis.

59 RARE SPECIES ADDED:
*BACILLUS DATABASE
B. aerius, B. altitudinis, B. azotoformans, B. clarkii, B. cohnii, B. funiculus, B. galliciensis, B. indicus, B. infernus, B. koreensis, B. niabensis, B. pseudalcaliphilus, B. wakoensis, B. invocatus, Paenibacillus alginolyticus, P. alkaliterrae, P. barengoltzii, P.dendritiformis, P elgii, P. favisporus and P. phyllosphaerae
*CORYNEBACTERIUM DATABASE
C. capitovis, C. halotolerans, C. hansenii, C. nuruki, C. terpenotabidum, Clavibacter michiganensis subsp. tessellarius,Arthrobacter woluwensis, Brevibacterium jeotgali, B. otitidis, B. pityocampae, Rathayibacter caricis, R. festucae, R. toxicus,Sanguibacter inulinus, S. keddieii, S. suarezii, Microbacterium aurantiacum, M. halophilum, M. hominis, M. lacticum and M. maritypicum.
*CAMPYLOBACTER & HELICOBACTER DATABASE
Campylobacter peloridis, Helicobacter anseris, H. brantae, H. cetorum, H. macacae, H. marmotae & H. mesocricetorum.
*CLOSTRIDIUM DATABASE
C. acidurici, C. aldenense, C. aminophilum, C. bolteae, C. citroniae and C. termitidis.
*ENTEROBACTERIACEAE DATABASE
Klebsiella singaporensis, Providencia vermicola, Serratia glossinae and Yersinia massiliensis.

Optimization of the "Target" mode, a function that allows the monitoring of a specific taxon during the identification process:after selecting the targeted taxon, the user is returned to "Input" page where test values (positive/negative/variable/unavailable) for the targeted taxon are listed on the left side.
Because there are taxons incompletely described in the literature, the identification databases may contain gaps. This lack of information may alter the identification results. To achieve the best identification, the input of the "unavailable" tests is blocked, so the user can only enter tests corresponding to a solid, well defined information in the database.
Utility function. The Input tests page will show on left side the global utility of the tests regarding the selected database, ranking *** high ** medium and * low. This will help the user to select the best performing tests and avoid consuming the lab resources with weak tests. See Analytics page for details about calculating the power of the tests.
New species added:
Dermabacter hominis - isolated from human blood cultures, abscesses, wounds, skin lesions, eyes.
Dermabacter vaginalis - isolated from human vaginal fluid.
Uruburuella suis - isolated from lesions of pneumonia and pericarditis in pigs.
Uruburuella testudinis - isolated from the pharynx, lung, liver, spleen, kidney and eye of tortoises of the genus Testudo.
Other changes
- Melibiose fermentation test was added to Staphylococcus database.
- Special characters used in some bacteria names from Pasteurella and Campylobacter databases were blocking the taxon to be selected in target mode. They were removed.
- A bug that was limiting the number of discriminatory tests sugested in "Details" page, was corrected.
- Minor biochemical adjustments to Gemella morbillorum, Streptococcus intermedius and Staphylococcus saccharolyticus.- The name of the taxon "Providentia stuartii" has been replaced with "Providencia stuartii".
- Added a message in "Details" page when no tests were used.
- Ads were removed from "Results" page.

1. Antibiogram interpreter changes: a duplicate input field in EUCAST-Enterobacteriaceae database was corrected.
2. Complete revision of ABIS Encyclopedia.
3. Changes in bacterial identification databases:
- Reorganisation of biochemical databases; tests are now listed in the same order with the input fields.
- Correction of Aeromonas veronii subsp. veronii "O129" test.
- Correction of Clostridium "neutral red" test.
- Correction of Vibrionaceae "Details" page error not showing the database version.
- Nine species added to Neisseria and Corynebacterium databases: Eikenella corrodens, Crenobacter luteus, Morococcus cerebrosus, Brevibacterium casei, Brevibacterium oceani, Brevibacterium paucivorans, and Brevibacterium sanguinis.

1. Changes in Bacterial Identification Databases:
Campylobacter jejuni subsp. jejuni was divided in 3 biotypes.
Thirteen Corynebacterium-like species from genera Arthrobacter, Brevibacterium, and Pseudoarthrobacter were added: Arthrobacter gandavensis, A. luteolus, A. nasiphocae, A. sanguinis, Pseudarthrobacter equi, P. scleromae, Brevibacterium avium, B. epidermidis, B. iodinum, B. linens, B. marinum, B. massiliense, and B. mcbrellneri.
2. Antibiogram interpreter changes:
Added CLSI disk diffusion criteria for antimicrobial susceptibility testing of Neisseria. Please note that EUCAST committee has not yet defined the disk diffusion criteria for antimicrobial susceptibility testing of Neisseria. Use the MIC breakpoints or the CLSI standard instead.Removed Vancomycin test from CLSI and Custom Staphylococcus databases.
Revision of Oxacillin test in Staphylococcus CLSI database and added Cefoxitin as surrogate for Oxacillin when testing S. aureus, S. lugdunensis and coagulase-negative staphylococci.
A CLSI Enterococcus database malfunction due to an error in input panel, was corrected.

Neisseria identification database including 34 species is now open for public use.
By adding another 6 coryneforms, the Corynebacterium database now exceeds 100 species.
The "Limits" menu was reorganized in "Analytics" menu and upgraded by a new function: "Similarity" that calculates the closeness between a specified taxon and its neighbors in the same taxonomic group (family or genus) based on their morphological and biochemical properties.
The "Target" function: a bug that was preventing the dropbox to list the taxa in ascending order, was removed.
Added Neisseriae: Neisseria animalis, N. animaloris, N. bacilliformis, N. canis, N. cinerea, N. dentiae, N. elongata subsp. elongata, N. elongata subsp. glycolytica, N. elongata subsp. nitroreducens, N. flavescens, N. iguanae, N. gonorrhoeae, N. lactamica, N. macacae, N. meningitidis, N. mucosa, N. polysaccharea, N. sicca, N. flava, N. perflava, N. subflava, N. shayeganii, N. wadsworthii, N. weaveri, N. zoodegmatis, Kingella denitrificans, K. kingae, K. oralis, K. potus, Alysiella crassa, Alysiella filiformis, Bergeriella denitrificans, Conchiformibius kuhniae, Conchiformibius steedae, and Simonsiella muelleri.
Added Coryneforms: Corynebacterium flavescens, Curtobacterium flaccumfaciens, Rhodococcus equi, Turicella otitidis, Pseudoglutamicibacter cumminsii, and Pseudoglutamicibacter albus.

"Target" function optimization: the user is better informed now, receiving the best tests for identification together with their values (positive/negative) for the targeted taxon. Identification criteria changes: D- & L- lactic acid tests were removed from Staphylococcus database and oxidative & fermentative metabolism tests were removed from Corynebacterium database.
14 Corynebacterium species added: C. accolens, C. auriscanis, C. atypicum, C. camporealensis, C. doosanense, C. epidermidicanis, C. felinum, C. kroppenstedtii, C. lubricantis, C. marinum, C. maris, C. massiliense, C. timonense & C. testudinoris.
Introducing salinicocci, a moderately halophylic group of microoorganisms, classified in Genus Salinicoccus, Family Staphylococcaceae; 11 species added: Salinicoccus albus, S. carnicancri, S. halodurans, S hispanicus, S. iranensis, S. jeotgali, S. kunmingensis, S qingdaonensis, S. roseus, S. sesuvii and S. siamensis.

ANDROID UPDATES:
"Bacterial identification" (ABIS online) application runs in landscape mode only.
"Results" page graphics were optimized for mobile browsers.
The last app version in Google Play is now 12.3 and is optimized for android version 5.

GENERAL CHANGES:
The minimal number of required test for identification was raised from seven to eight.
The default identification mode is now set to "Auto". This mode will let the computer decide which mode to use, "Normal" or "Forced". These modes can still be manually set if the user consider to be necesary.
Eight AEROCOCCUS species added in Streptococcus identification database: Aerococcus christensenii, A. sanguinicola, A. suis, A. urinae, A. urinaeequi, A. urinaehominis, A. vaginalis, and A. viridans.
Three species added to genus Corynebacterium: C. humireducens, C. mustelae and C. mastitidis.

GENUS STREPTOCOCCUS
Species added: Streptococcus cuniculi, S. dentasini, S. dentisani, S. hongkongensis, S. loxodontisalivarius, S. moroccensis, S.oriloxodontae, S. orisasini, S. rifensis, and S. saliviloxodontae.
Biochemical tests revison of Streptococcus merionis, S. ovis, S. oralis, S. gallinaceus, S. criceti, S. pseudopneumoniae, and S. pseudoporcinus.

GENUS CORYNEBACTERIUM
H2S production and indole production were removed as they have little significance for identification. They were replaced by ribose fermentation and alpha-glucosidase activity tests.
Species added: Corynebacterium caspium, C. ciconiae, C. efficiens, C. frankenforstense, C.breiburgense, C. glaucum, C. lactis.
OTHER CHANGES
Listeria, Campylobacter, and Helicobacter 16S RNA databases added to molecular identification.
Graphical optimization.

The "Target" mode; a new function that allows the monitoring of a specific taxon during the identification process has been revised and upgraded. The recommended tests for the identification and the unsuitable tests (weak, delayed, variable) are listed for the selected taxon."Results" and "Biochemical details" pages, displaying the distance from the top ranked taxa, the matching percent of biochemical tests and their suitability, remain unchanged.The "Tests power" option evaluates the biochemical tests, providing information about discrimination power, variability and reliability. Reviewing these performance indicators may be useful for selecting the best tests or minimal requirement for identification.
20 species were added to Corynebacterium database and ABIS Encyclopedia: C. appendicis, C. aquilae, C. canis,C. confusum, C. casei, C. coyleae, C. durum, C. falsenii, C. freneyi, C. imitans, C. lipophiloflavum, C. mooreparkense, C. mucifaciens, C. nigricans, C. riegelii, C. simulans, C. singulare, C. sundsvallense, and C. thomssenii.

1. Adding Corynebacterium identification database;
2. Introduction of Lancefield grouping for streptococci and related genera (enterococci, lactococci et al.);
3. Reorganization of Encyclopedia's index for an easier access;
4. Complete revision of the entire Streptococcus genus, 10 more biochemical tests (fermentation of: dulcitol, galactose, glycerol, glycogen, inositol, maltose, melibiose, melezitose, salicin, and starch), and 13 new Streptococcus species added to identification database: S. dentapri, S. devriesei, S. intermedius, S. lactarius, S. porci, S. porcorum, S. rubneri, S. rupicaprae, S. sinensis, S. tigurinus, S. troglodytae, S. troglodytidis and S. ursoris.
Other changes:
- Streptococcus pleomorphus Barnes et al. 1977 was moved to genus Faecalicoccus as Faecalicoccus pleomorphus De Maesschalck et al. 2014.
- Streptococcus bovis is now included in S. equinus species.
- Streptococcus mitior was removed as is no longer considered a valid name.

GENERAL CHANGES:
Added transparent graphics for navigation buttons.
Revision of A+T/C+G page.

BIOCHEMICAL IDENTIFICATION
a) 5 species were added to Helicobacter database: Helicobacter pametensis, H. rodentium, H. suis, H. typhlonius & Helicobacter sp. strain CLO-3.
b) Acinetobacter rudis was added to Non-fermenters database. Revision of Acinetobacter haemolyticus, A. venetianus, A. beijerinckii & A. gyllenbergii.
c) 16 species were added to Lactobacillus database: Lactobacillus saerimneri, L. sakei, L. saniviri, L. senioris, L. secaliphilus, L. suntoryeus, L. senmaizukei, L. similis, L. sucicola, L. taiwanensis, L. ultunensis, L. vaginalis, L. versmoldensis, L. zymae, L. xiangfangensis and L. vini.

Announcing the end support for "ABIS online" Android application 1118532 by October 01.
A newer version is available in Google Play Store at https://play.google.com/store/apps/details?id=net.tgw1916. Changes from previous version: zoom option added (double tap for zoom in/out), hardware "back" button is no longer returning to home page, refresh button added (cache clearing is no longer required for updates to be effective). Requirements: Android 4.4 or lower; all devices are supported.
2 species were added to Campylobacter/Helicobacter database: Helicobacter muridarum and Helicobacter mustelae.
13 species were added to Lactobacillus database: Lactobacillus ozensis, L. panis, L. pantheris, L. paracollinoides, L. parakefiri, L. paralimentarius, L. parafarraginis, L. parabuchneri, L. ferintoshensis, L. paucivorans, L. pobuzihi, L. rhamnosus, L. rossiae and L. rennini.

GENERAL UPDATES
The search engine for somatic and flagellar antigens in Kauffman-White scheme (Salmonella) was improved.

MOLECULAR IDENTIFICATION (not available on Android mobile application).
Optimization of ARN 16S gene identification engine, speed being significantly faster now.
Bacillaceae molecular database, including 396 species in 52 genera, is now open for public use.

BIOCHEMICAL IDENTIFICATION

FAMILY ENTEROBACTERIACEAE: revision of Salmonella nomenclature.
GENUS LACTOBACILLUS: 14 species added: Lactobacillus helveticus,Lactobacillus hilgardii, Lactobacillus homohiochii, Lactobacillus hordei, Lactobacillus ingluviei, Lactobacillus intestinalis, Lactobacillus kalixensis, Lactobacillus kefiranofaciens subsp. kefiranofaciens, Lactobacillus kefiranofaciens subsp. kefirgranum, Lactobacillus kitasatonis, Lactobacillus kisonensis, Lactobacillus otakiensis, Lactobacillus rapi and Lactobacillus sunkii.

GENUS HELICOBACTER: gelatin, casein and starch hydrolysis tests were removed due to their low significance for identification.
Gamma-glutamyl-transferase test was added.
3 species added: Helicobacter hepaticus, Helicobacter ganmani and Helicobacter cholecystus.

PSEUDOMONAS DATABASE
Several biochemical tests were updated in Moraxella bovis.
13 species added: Moraxella caprae, M. lacunata, M. equi, M. caviae, M. cuniculi, M. lincolnii, M. nonliquefaciens, M. osloensis, M. ovis, M. pluranimalium, M. porci, Oligella ureolytica, Oligella urethralis.

CLOSTRIDIUM DATABASE
Several biochemical tests were updated in Clostridium clostridioforme.
16 species added:Clostridium psychrophilum, C. puniceum, C. putrefaciens, C. ramosum, C. rectum, C. saccharobutylicum, C. roseum, C. saccharolyticum, C. saccharoperbutylacetonicum, C. sardiniense, C. sartagoforme, C. scatologenes, C. scindens, C. sphenoides, C. spiriforme, and C. sporosphaeroides.

PSEUDOMONAS DATABASE
Tests changes: 'Denitrification' was replaced with 'Nitrates reduction'. 'Number of flagella' was replaced with 'Motility'. Biochemical tests have been revised accordingly.
Tests added: 'growth on MacConkey agar', 'Indole production', 'H2S production', 'Lysine decarboxylase', 'Ornithine decarboxylase' and 'Alkaline phosphatase'.
7 species added: Riemerella (Moraxella) anatipestifer, Riemerella columbina, Moraxella atlantae, Moraxella bovis, Moraxella boevrei, Moraxella bovoculi and Moraxella canis.

CLOSTRIDIUM DATABASE
25 species added: Cellulosilyticum (Clostridium) lentocellum, Cellulosilyticum ruminicola, Flavonifractor plautii, Clostridium ghonii, C. glycolicum, C. herbivorans, C. hiranonis, C. hylemonae, C. indolis, C. innocuum, C. intestinale, C. isatidis, C. lacusfryxellense, C. limosum, C. lituseburense, C. malenominatum, C. mangenotii, C. nexile, C. oceanicum, C. oroticum, C. papyrosolvens, C. paraputrificum, C. pascui, C. pasteurianum and C. propionicum.

GENERAL CHANGES
We are proud to announce new ABIS services:
The most important is the “Salmonella serovars identification”. Based on updated Kauffman-White scheme, a 2570 Salmonella serovars database is now available. It can be accessed from the “Log in” page.
Second service: we have added a 'Software limitations' page where all identifiable taxa are listed and biochemical tests are evaluated. This service evaluates the biochemical tests, providing information about discrimination power, variability and reliability. Reviewing these performance indicators may be useful for selecting the best tests or minimal requirements for identification. It can be accessed from the biochemical identification main page.
Third: the molecular identification has now the A+T / G+C ratio calculation option.
Others: the 16S rRNA gene identification engine has been optimized for the increasing of computing speed.>BACILLUS DATABASE
Minor bichemical changes for Brevibacillus brevis, Paenibacillus thiaminolyticus and Paenibacillus amylolyticus.
Species added: Paenibacillus validus, Paenibacillus panacisoli, Paenibacillus pectinilyticus, Paenibacillus prosopidis, Paenibacillus provencensis, Paenibacillus urinalis, Paenibacillus residui, Paenibacillus sputi, Paenibacillus turicensis, Paenibacillus wynnii, Brevibacillus agri, Brevibacillus centrosporus & Brevibacillus parabrevis.

GENERAL CHANGES
Printing optimisation: when printing out the results, the fonts are enlarged for a better visibility on paper. The printer icon is located in 'Biochemical details' page. For best contrast, we recommend the printer to be set on 'black and white' finishing.
Introducing 'Strain name / code' function. Strain name or code will appear both on 'Biochemical details' page and paper-printed results.

BACILLUS DATABASE
According to Genersch et al. 2006, P. larvae subsp. pulvifaciens and P. larvae subsp. larvae are reclassified as a single species: Paenibacillus larvae. ABIS database has been updated with taxonomic and biochemical changes and P. larvae subsp pulvifaciens was removed from database.
Paenibacillus macquariensis - contains two subspecies: P. macquariensis subsp. defensor and P. macquariensis subsp. macquariensis. Important biochemical changes have been applied.
Species added: Paenibacillus chinjuensis, P. chondroitinus, P. alginolyticus, P. cookii, P. contaminans, P. curdlanolyticus, P. glucanolyticus, P. graminis, P. jamilae, P. konsidensis, P. koreensis, P. kribbensis, P. terrae, P. lactis, P. lautus, P. peoriae, P. massiliensis, P. sanguinis, P. timonensis, P. mendelii, P. montaniterrae, P. siamensis, P. septentrionalis, P. nanensis, P. thailandensis, P. naphthalenovorans, P. nematophilus, P. odorifer and P. pabuli.

New genus added: Aeromonas
For a more easy and reliable identification, and considering the ecological niche, biochemical tests, pathogenicity and cultural characters, Genus Aeromonas was included in Vibrio database.

VIBRIO DATABASE
New identification test added: “resistance to O/129 vibriostatic agent”.
Species added: Vibrio rhizosphaerae, Vibrio rumoiensis, Vibrio sagamiensis and Vibrio superstes.

BACILLUS DATABASE
Minor biochemical changes for Paenibacillus apiarius and Paenibacillus alvei.
Species added: Paenibacillus assamensis, Paenibacillus azoreducens, Paenibacillus aestuarii, Paenibacillus barcinonensis, Paenibacillus borealis , Paenibacillus brasilensis, Paenibacillus castaneae, Paenibacillus chibensis , Paenibacillus illinoisensis, Paenibacillus cellulositrophicus and Paenibacillus cineris.

GENERAL UPDATES
Android market - ABIS can now be installed on mobile devices. Search 'Google Play Store' from your Android OS phone or tablet PC.
Graphical optimizations for increasing loading speed.
Introduced a direct link to species description in "Details" page. By clicking on magnifying glass from the vicinity of identified species, details about taxonomy, ecology, pathogenicity, biochemical and morpho-cultural characters are revealed.
Implemented a warning button in 'Results' page for highlighting supplementary species-specific morpho-biochemical characters that may be useful for identification.

CAMPYLOBACTER DATABASE
New tests added: alkaline phosphatase, growth with triphenyl-tetrazolium chloride, growth with cefoperazone (64 mg/L), and growth on MacConkey agar.
Species added: Arcobacter cibarius, A. halophilus, A. marinus, A. mytili and A. thereius.

BACILLUS DATABASE
New tests added: melezitose, melibiose, raffinose and rhamnose acidification.
Species added: Bacillus aidingensis, Bacillus canaveralius, Bacillus cibi, Bacillus horneckiae, Bacillus isabeliae, Bacillus korlensis, Lysinibacillus massiliensis, Bacillus muralis, Bacillus neizhouensis, Bacillus oshimensis, Bacillus polygoni, Bacillus ruris, Bacillus safensis, Bacillus siamensis, Bacillus tequilensis and Bacillus trypoxylicola.

Elevation of ABIS online to version 8, featuring the Campylobacter database. What is new in ABIS 8:

CAMPYLOBACTER DATABASE
Campylobacter and Arcobacter biochemical identification (for the list of identifiable species see ABIS Encyclopedia).

ENTEROBACTERIACEAE DATABASE
Pantoea stewartii has been changed to P. stewartii subsp. stewartii as a consequence of Pantoea stewartii subsp. indologenes description by Mergaert et al. 1993.
Xenorhabdus luminescens Thomas and Poinar 1979 has been transferred to genus Photorhabdus as Photorhabdus luminescens by Boemare 1993.
Proteus myxofaciens Cosenza and Podgwaite 1966 has been transferred to genus Cosenzaea as Cosenzaea myxofaciens (Cosenza and Podgwaite 1966) Giammanco et al. 2011.
Shigella boydii, S. dysenteriae and S. flexneri are now treated as individual taxons.
Biochemical tests have been revised.
Important biochemical changes for genus Xenorhabdus.
Species added: Pantoea stewartii subsp. indologenes, Photorhabdus asymbiotica subsp. asymbiotica, Photorhabdus asymbiotica subsp. australis, Photorhabdus luminescens subsp. akhurstii, Photorhabdus luminescens subsp. laumondii, Photorhabdus temperata, Providencia burhodogranariea, Providencia sneebia, Serratia nematodiphila, Serratia ureilytica, Tatumella citrea, Tatumella punctata, Tatumella terrea, Tatumella morbirosei, Trabulsiella odontotermitis, Xenorhabdus japonica, Yersinia similis, Enteric Group 58, Enteric Group 60, Enteric Group 68 and Enteric Group 69.
Molecular 16S RNA database for Enterobacteriaceae is now available.

BACILLUS DATABASE
Species added: Bacillus sporothermodurans, Bacillus subterraneus, Bacillus thermoamylovorans, Bacillus vietnamensis, Bacillus acidiceler and Bacillus acidiproducens.
Minor biochemical changes for several other Bacillus species.

ENTEROBACTERIACEAE DATABASE
Escherichia blattae Burgess et al. 1973 was transferred to genus Shimwellia as Shimwellia blattae Priest and Barker 2010.
Obesumbacterium proteus biogroup 2 was transferred to genus Shimwellia as Shimwellia pseudoproteus.
Hafnia alvei biogroup 1 and Obesumbacterium proteus biogroup 1 may be considered synonyms.
Klebsiella planticola and Klebsiella terrigena were transferred to genus Raoultella as Raoultella planticola and Raoultella terrigena, respectively.
Species added: Shimwellia pseudoproteus (Obesumbacterium proteus biogroup 2), Hafnia alvei biogroup 1, (Obesumbacterium proteus biogroup 1), Raoultella ornithinolytica, Kluyvera georgiana, Salmonella enterica subsp. enterica serovar Choleraesuis, Salmonella enterica subsp. enterica serovar Gallinarum, Salmonella enterica subsp. enterica serovar Paratyphi A, Salmonella enterica subsp. enterica serovar Pullorum, Salmonella enterica subsp. enterica serovar Typhi, Morganella morganii subsp. sibonii and Morganella morganii biogroup 1.

BACILLUS DATABASE
Species added: Rummeliibacillus pycnus, Bacillus oleronius, Bacillus shackletonii, Solibacillus silvestris, Bacillus siralis and Bacillus smithii.

BACILLUS DATABASE
Species added: Bacillus marisflavi, Bacillus methanolicus, Bacillus nealsonii, Viridibacillus neidei, Bacillus niacini, Bacillus novalis, Bacillus soli and Bacillus vireti.

ENTEROBACTERIACEAE DATABASE
Enterobacter intermedius was transferred to genus Kluyvera as Kluyvera intermedia.
Enterobacter sakazaki was transferred to genus Cronobacter as Cronobacter sakazakii.
Enterobacter agglomerans was transferred to genus Pantoea as Pantoea agglomerans.
Erwinia nigrifluens, Erwinia quercina, Erwinia rubrifaciens and Erwinia salicis were transferred to genus Brenneria as Brenneria nigrifluens, B. quercina, B rubrifaciens and B. salicis, respectively (Hauben et al. 1999).
Pectobacterium chrysanthemi was transferred to genus Dickeya as Dickeya chrysanthemi (Samson et al. 2005).
Erwinia paradisiaca was transferred to genus Dickeya as Dickeya paradisiaca (Samson et al. 2005).
Erwinia carotovora was transferred to genus Pectobacterium as Pectobacterium carotovorum.
Erwinia ananas was transferred to genus Pantoea as Pantoea ananatis.
Erwinia stewartii was transferred to genus Pantoea as Pantoea stewartii.
Erwinia uredovora is a synonym of Erwinia ananas and was transferred to genus Pantoea as Pantoea ananatis.
Erwinia dissolvens was transferred to genus Enterobacter as Enterobacter dissolvens (Rosen 1922) Brenner et al. 1988.
Erwinia cypripedii was transferred to genus Pantoea as Pantoea cypripedii.
Biochemical changes for several species.
Species added: Buttiauxella brennerae, Buttiauxella ferragutiae, Buttiauxella gaviniae, Buttiauxella izardii, Buttiauxella noackiae, Buttiauxella warmboldiae, Cedecea species 3, Cedecea species 5, Citrobacter braakii, Citrobacter farmeri, Citrobacter gillenii, Citrobacter murliniae, Citrobacter rodentium, Citrobacter sedlakii, Citrobacter werkmanii, Citrobacter youngae, Edwardsiella tarda biogroup 1, Enterobacter pyrinus, Brenneria alni, Dickeya paradisiaca, Pectobacterium atrosepticum, Pectobacterium betavasculorum, Pectobacterium wasabiae, Erwinia aphidicola, Erwinia billingiae, Erwinia pyrifoliae and Erwinia toletana.

GENUS BACILLUS
Bacillus insolitus was moved to genus Psychrobacillus as Psychrobacillus insolitus.
Minor biochemical changes for several species.
Strains of B. carotarum , B. capri, B. cobayae, B. musculi, B. maroccanus, and B. macroides were reclassified as Bacillus simplex by Heyrman et al. 2005.
Species added: Salimicrobium halophilum, Bacillus horti, Bacillus hwajinpoensis, Bacillus jeotgali, Bacillus krulwichiae, Bacillus luciferensis.
An erroneous scripting line for xylose test was corrected. The line was responsible only for showing unmatching xylose test so, the previous identifications were not afected.

GENUS STAPHYLOCOCCUS
Staphylococcus pulvereri Zakrzewska-Czerwinska et al. 1995 is a later synonym of Staphylococcus vitulinus.
Staphylococcus sciuri includes subspecies sciuri, rodentium & carnaticus.
Minor biochemical changes for several species.
Species added: Staphylococcus microti, Staphylococcus pasteuri, Staphylococcus succinus subsp. succinus, Staphylococcus succinus subsp. casei,
Species added in genus Macrococcus: Macrococcus bovicus, Macrococcus brunensis, Macrococcus carouselicus, Macrococcus equipercicus, Macrococcus hajekii, Macrococcus lamae.

GENUS STAPHYLOCOCCUS
Staphylococcus caseolyticus was moved to genus Macrococcus as Macrococcus caseolyticus.
New species added: Staphylococcus equorum subsp. linens
Staphylococcus equorum changed to Staphylococcus equorum subsp. equorum
Staphylococcus and Streptococcus interface optimization
Biochemical changes for several species.

GENUS BACILLUS
New species added: Bacillus endophyticus, Bacillus farraginis, Bacillus fordii, Bacillus fortis, Bacillus flexus, Bacillus fumarioli, Bacillus galactosidilyticus, Bacillus gelatini, Lysinibacillus fusiformis

GENUS PSEUDOMONAS
Chryseomonas polytricha Holmes et al. 1986 is a later heterotypic synonym of Pseudomonas luteola Kodama et al. 1985.

GENUS BACILLUS
Bacillus laterosporus was moved to genus Brevibacillus.
Bacillus apiarius was moved to genus Paenibacillus.
"Capsule" is no longer an identification criteria in Bacillus database.
New species added: Bacillus aeolius, B. alcalophilus, B. amyloliquefaciens, B. asahii, B. aquimaris, B. barbaricus, B. bataviensis, B. benzoevorans, B. carboniphilus, B. decolorationis, B.drentensis.

GENUS ENTEROCOCCUS
New species added: Enterococus gilvus, E. palens, E. thailandicus.
Genus Streptococcus
New species added: Streptococcus cristatus, S. dentirousetti.

FAMILY VIBRIONACEAE
New species added: Enterovibrio nigricans, Salinivibrio siamensis, Vibrio aerogenes, Vibrio agarivorans, Vibrio cyclitrophicus, Vibrio hepatarius, Vibrio inusitatus, Vibrio kanaloae, Vibrio pacinii, Vibrio pomeroyi, Vibrio porteresiae, Vibrio rarus, Vibrio rotiferianus, Vibrio ruber, and Vibrio sinaloensis.

Major update:
A new section based on molecular 16S rRNA sequence analysis is now available. For details and technical info see ‘Help’ page.
Changes:
H.agni and H. somnus may be synonyms of Histophilus somnis (formerly Histophilus ovis). De Ley et al., (1990) suggested that H. agni and H. somnus to be transferred to genus Histophilus, Histophilus ovis being a senior synonym for H. agni. However, none of these names are valid, and no types have been designated.
Pasteurella langaa changed to P. langaaensis (corrected by Trüper and De' Clari 1998).
Pasteurella trehalosi changed to Biberstenia trehalosi.
New species included in ABIS databases:
Phocoenobacter uteri, Volucribacter amazonae, Volucribacter psittacicida, Streptococcus caballi, Streptococcus castoreus, Streptococcus didelphis, Streptococcus entericus, Streptococcus henryi, Streptococcus ictaluri, Streptococcus lutetiensis, Streptococcus massiliensis, Streptococcus merionis, Vibrio neptunius, Vibrio brasiliensis, Vibrio xuii, Vibrio crassostreae, Vibrio lentus, Vibrio gallicus, Salinivibrio proteolyticus.
General updates:
A scripting error that was causing wrong buttons’ placement in ‘Results’ page, triggered by ‘Insufficient tests’ message, has been corrected.

New species included in ABIS databases:

Vibrionaceae database: Enterovibrio norvegicus.

Streoptococcus database: S gallolyticus subsp. macedonicus, S gallolyticus subsp. pasteurianus and 32 species from genus Enterococcus (for species list see ABIS encyclopedia).
Changes:Streptococcus intestinalis and Streptococcus alactolyticus are considered synonyms (S. intestinalis was removed).
S. bovis & S. equinus may be considered synonyms (they are still treated as separate entities by ABIS online).
Removed tests: growth at pH 9.6, growth with 40% bile, growth with 0.25% optochin and acid production from salicin.
Added tests: acid production from amygdalin, arabinose, arbutin, fructose, glucose, mannose, rhamnose, sucrose and xylose.

General updates:
- graphics were optimized for faster loading,
- the software is now suggesting the required tests to differentiate the first two identified species, if the id% is smaller than 1% and if there are available tests.

Vibrio damsela changed to Photobacterium damselae.
Pasteurella piscicida changed to Photobacterium damselae subsp.piscicida, consequently Photobacterium damselae changed to Photobacterium damselae subsp. damselae.
New entries in ABIS databases:
a) Enterobacteriacaeae: Plesiomonas shigelloides.
b) Vibrionaceae: Photobacterium leiognathi, Photobacterium angustum, Photobacterium phosphoreum, Vibrio navarrensis, Vibrio scophthalmi, Vibrio splendidus I, Vibrio splendidus II, Vibrio tapetis, Vibrio tubiashii.
c) Pseudomonas and Pseudomonas-like: Burkholderia plantarii, Burkholderia graminis, Pseudomonas fuscovaginae, P. koreensis, P. jinjuensis, P. lini, P. lutea, P. marincola, P. moraviensis, P. mosselii, P. nitroreducens, P. panacis, P. pachastrellae, P. proteolytica, P. pohangensis, P. pertucinogena, P. rhizosphaerae, P. simiae, P. taetrolens.

We are proud to announce the long-awaited release of ABIS 7 online. Since the ABIS 6 is history, you should know what is new in ABIS 7:
1 Vibrionaceae family including Vibrio, Aliivibrio, Listonella & Grimontia genera (35 identifiable species out of 42) is now available for public use. For the species list visit the Encyclopedia section.
2 Data-entering pannels for Pseudomonas and Enterobacteriaceae databases was redesigned for an easier usage.
3 Pseudomonas database was updated:
a) 13 identification morphobiochemical characters added: urea hydrolysis, esculin hydrolysis, ONPG (beta-galactosidase), utilization as carbon source of citrate, L-arabinose, mannose, mannitol, sorbitol, sucrose, xylose & fructose, growth at 4 ºC, number of flagella.
b) 24 more Pseudomonas and Pseudomonas-like species added:Pseudomonas migulae, Pseudomonas lurida, Pseudomonas lundensis, Pseudomonas libanensis, Pseudomonas indica, Burkholderia glumae, Pseudomonas grimontii, Pseudomonas graminis, Pseudomonas gessardii, Burkholderia andropogonis, Pseudomonas peli, Pseudomonas borbori, Pseudomonas asplenii, Pseudomonas agarici, Pseudomonas meridiana, Pseudomonas cremoricolorata, Pseudomonas frederiksbergensis, Pseudomonas extremorientalis, Pseudomonas citronellolis, Pseudomonas brassicacearum, Pseudomonas thivervalensis, Pseudomonas argentinensis, Pseudomonas antarctica.
c) Pseudomonas saccharophila Doudoroff 1940 changed to Pelomonas saccharophila Xie and Yokota 2005.

PASTEURELLACEAE DATABASE
New member added: Actinobacillus arthritidis.
Pasteurella granulomatis changed to Mannheimia granulomatis.
Pasteurella haemolytica biogroup 1 changed to Mannheimia haemolytica.
Pasteurella haemolytica biovar T changed to Pasteurella trehalosi.
Pasteurella haemolytica biogroup 3 and 5 changed to Mannheimia glucosida.
Mannheimia (Pasteurella) haemolytica bigroup 6 changed to Mannheimia varigena.
Actinobacillus actinomycetemcomitans changed to Aggregatibacter actinomycetemcomitans.

STREPTOCOCCUS DATABASE
New members added: Streptococcus plurextorum, Streptococcus pluranimalium, Streptococcus phocae, Streptococcus parasanguinis, Streptococcus ovis, Streptococcus orisuis, Streptococcus orisratti, Streptococcus australis and Streptococcus oligofermentans.

GENERAL FIXES: identification engine improvement
1. Identification formula in "Normal mode" was changed, id% beeing now 3 to 7% higher. "Forced mode" formula was not modified. Results before June 1st may have minor variations.
2. Taxa below 75% probability are no more listed, returning now "Taxon not found" message. Change was made in order to avoid confusions when user is trying to identify a taxon that is not included in database.

STREPTOCOCCUS DATABASE
Accuracy results corrected.
Streptococcus hansenii changed to Blautia hansenii.
S. alactolyticus and S. intestinalis are synonyms but are still treated as individual species.
S. equinus and S. bovis are synonyms but are still treated as individual species.
S shiloi was included in S. iniae as they are considered synonyms.
S.difficilis was included in S. agalactiae as they are considered synonyms.
Alpha hemolysis on sheep blood agar of Streptococcus iniae (S. shiloi) was set to 'negative' (admin decision).
S. milleri, S. constellatus and S. intermedius are now included in S. anginosus.
S. waius and S. macedonicus were changed to S. gallolyticus subsp. macedonicus.
S. infantarius subspecies infantarius & coli are presented as a single taxon: S. infantarius.
S. mitis was splitted into biotype 1 & biotype 2
S. mitior was included in S. mitis biotype 1 due to S. mitior invalid name and a very close biochemical resemblance with S. mitis biotype 1 (admin decision).
S. cricetus changed to S. criceti (Trüper and De’ Clari 1998)
New species added: S. minor, S. marimammalium, S. infantis / peroris, S. infantarius, S. hyovaginalis, S. halichoeri, S. gallinaceus, S. equi subsp. ruminatorum, S. gallolyticus (S. caprinus).

ENTEROBACTERIACEAE DATABASE
Corrected a wrong displaying of rhamnose expected test in Biochemical details section.
Salmonella choleraesuis species changed to Salmonella enterica.

General fixes:
A hyperlink-video overlapping error encountered by Mozilla-Firefox users was fixed.

BACILLUS DATABASE
B. cereus, B. thuringiensis and B.mycoides are now treated as separate entities.
B. globisporus changed to Sporosarcina globispora,
B. pasteurii changed to Sporosarcina pasteurii,
B. psychrophilus changed to Sporosarcina psychrophila,
B. aneurinolyticus changed to Aneurinibacillus aneurinilyticus,
B. pantothenicus changed to Virgibacillus pantothenicus,
Paenibacillus pulvifaciens changed to P. larvae subsp. pulvifaciens and consequently P. larvae changed to P. larvae subsp. larvae,
7 other Bacillus species moved to genus Paenibacillus,
2 Bacillus species moved to genus Brevibacillus,
B. chitinosporus and B. rarerepertus were eliminated from database,
Bacillus cirroflagelosus was included in database.
Biochemical tests updated for several Bacillus species.
Beta testing is over; Bacillus identification is running at full capacity.

PSEUDOMONAS DATABASE
Gelatin hydrolysis and 2-Ketogluconate utilization test corrected for C. luteola
Stentrophomonas maltophilia corrected to Stenotrophomonas maltophilia
Staphylococcus database:
"Growth at 15 ºC" character of Staphylococcus schleiferi subsp. coagulans and Staphylococcus schleiferi subsp. schleiferi was set to "negative" (admin decision).