(c) Costin Stoica
'Step by step' identification scheme for Salmonella
gallinarum - pullorum. Contains isolation and
identification requirements based on SR EN ISO
6579 and frequently used media.
Also, may be extended to other Salmonella serovars.
Measured diameter of the inhibition zones are
compared with the standard values and sorted in
descending order of antibacterial activity (also bacterial
strain is marked as 'susceptible', 'intermediate' or
'resistant' accordingly to the used antibiotics).
Software database contains CLSI and EUCAST
recommended antibiotics for Enterobacteriaceae,
Pasteurellaceae, Enterococcus, Neisseria,
Staphylococcus, Streptococcus and Pseudomonas.
The ABIS project started in 2003 as a MS-DOS-based
program for clinically significant Enterobacteriaceae
identification, using biochemical characters, morphology,
growth conditions, ecology & pathogenicity data.
In one year program reached version 4, upgrading with 3
more genera: Bacillus, Streptococcus & Staphylococcus.
ABIS 5 was released in 2005 with major changes
including an easy to use Windows based interface, step
by step identification for Gram positive & negative bacteria,
rods / cocci (Genera only), identification maps and 2 more
families included: Pasteurellaceae (Pasteurella,
Actinobacillus, Haemophilus) & Pseudomonadaceae.
Also, Bacillus, Enterobacteriaceae & Staphylococcus
databases were updated and the biochemical tests list,
used for identification, was extended.
Continuous changes in bacterial taxonomy forced us to
stop the development of the stand-alone version and
focus on the online development. In the year 2007 "ABIS 6
online", linked with a germs Encyclopedia and hosted by
'Regnum Prokaryotae" site, was officially released for
public use. Later, ABIS 7 brought Vibironaceae
identification, ABIS 8 - family Campylobacteraceae and
ABIS 9 - genus Clostridium and Salmonella
Kauffman-White scheme. Other upgrades followed up to
actual ABIS: Helicobacter and Lactobacillus (ABIS 10),
Listeria and Erysipelothrix (ABIS 11), Corynebacterium
and Lancefield grouping for streptococci (ABIS 12),
Neisseria and Chromobacterium (ABIS13). Databases
are periodically revised and updated following the latest
changes in bacterial domain.
Salmonella isolation &
ABIS online, Antibiogram interpreter and Regnum
Prokaryotae site are also available as free Android
applications in Google Play Store.
ABIS online - Advanced Bacterial Identification Software is a laboratory tool for bacterial identification,
based on morpho-biochemical characters, cultural characteristics, growth conditions, ecology and
pathogenicity data. The program allows a great flexibility in choosing biochemical tests and is an
alternative to commercial systems or code-books. The software is linked with a germs Encyclopedia and
an antibiogram interpreter.