| Chocolate agar |
| Sheep blood agar |
QUALITY CONTROL
Following germs should have a good growth at 35 °C in 24 hours:
Neisseria meningitidis ATCC 13090,
Streptococcus pneumoniae ATCC 6305,
Streptococcus pyogenes ATCC 19615,
Bacillus cereus ATCC 11778.
Following germs should have a good growth at 28 °C in 3 days:
Candida albicans ATCC 10231,
Aspergillus niger ATCC 16404.
Following germs should have a good growth at 35 °C in 24 hours:
Neisseria meningitidis ATCC 13090,
Streptococcus pneumoniae ATCC 6305,
Streptococcus pyogenes ATCC 19615,
Bacillus cereus ATCC 11778.
Following germs should have a good growth at 28 °C in 3 days:
Candida albicans ATCC 10231,
Aspergillus niger ATCC 16404.
COMPOSITION
Nutrient agar composition:
Beef extract .......................................................................10g
Peptone .............................................................................10g
NaCl .....................................................................................5g
Agar ...................................................................................20g
Add distilled water 1000ml; adjust pH to 7.2 at 25 ºC. Sterilize by
autoclaving at 121 ºC for 15 minutes.
Melt nutrient agar base, cool to 45-50 ºC then add 5% sterile, defibrinated
blood (sheep, bovine or horse origin). For Haemophilus spp. and
Neisseria spp. prepare chocolate agar by adding blood at higher
temperature (70-80 ºC).
Nutrient agar composition:
Beef extract .......................................................................10g
Peptone .............................................................................10g
NaCl .....................................................................................5g
Agar ...................................................................................20g
Add distilled water 1000ml; adjust pH to 7.2 at 25 ºC. Sterilize by
autoclaving at 121 ºC for 15 minutes.
Melt nutrient agar base, cool to 45-50 ºC then add 5% sterile, defibrinated
blood (sheep, bovine or horse origin). For Haemophilus spp. and
Neisseria spp. prepare chocolate agar by adding blood at higher
temperature (70-80 ºC).
DESCRIPTION
Highly nutritive medium used for the isolation, cultivation and hemolytic ability testing of various fastidious
microorganisms.
Highly nutritive medium used for the isolation, cultivation and hemolytic ability testing of various fastidious
microorganisms.
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