QUALITY CONTROL
Salmonella typhimurium ATCC 14028: good growth; red (same colour as the medium / translucent) colonies with
black center, Escherichia coli ATCC 25922: yellow colonies, Shigella flexneri ATCC 12022: colorless colonies,
Enterobacter cloacae ATCC 13047: yellow colonies.
Salmonella typhimurium ATCC 14028: good growth; red (same colour as the medium / translucent) colonies with
black center, Escherichia coli ATCC 25922: yellow colonies, Shigella flexneri ATCC 12022: colorless colonies,
Enterobacter cloacae ATCC 13047: yellow colonies.
COMPOSITION
Yeast extract ............................................................................... 3.0 g
L-Lysine HCl ............................................................................... 5.0 g
Xylose ....................................................................................... 3.75 g
Lactose ....................................................................................... 7.5 g
Sucrose ...................................................................................... 7.5 g
Sodium desoxycholate ................................................................ 1.0 g
Sodium chloride .......................................................................... 5.0 g
Sodium thiosulphate ................................................................... 6.8 g
Ferric ammonium citrate ............................................................. 0.8 g
Phenol red ................................................................................ 0.08 g
Agar ......................................................................................... 13.5 g
Distilled water ad 1000ml; adjust pH to 7.4 ± 0.2. Do not overheat.
Yeast extract ............................................................................... 3.0 g
L-Lysine HCl ............................................................................... 5.0 g
Xylose ....................................................................................... 3.75 g
Lactose ....................................................................................... 7.5 g
Sucrose ...................................................................................... 7.5 g
Sodium desoxycholate ................................................................ 1.0 g
Sodium chloride .......................................................................... 5.0 g
Sodium thiosulphate ................................................................... 6.8 g
Ferric ammonium citrate ............................................................. 0.8 g
Phenol red ................................................................................ 0.08 g
Agar ......................................................................................... 13.5 g
Distilled water ad 1000ml; adjust pH to 7.4 ± 0.2. Do not overheat.
DESCRIPTION
Medium used for isolation and identification of Salmonella and Shigella. H2S-positive germs form black colonies.
Fermentation of xylose, lactose and sucrose results in yellow colonies, sometimes together with medium yellowing
around colonies. Lysine decarboxylation produces a purple colouration around the colonies.
Composition formula is included in SR EN ISO 6579 standard "Horizontal method for the detection of Salmonella
spp.".
Medium used for isolation and identification of Salmonella and Shigella. H2S-positive germs form black colonies.
Fermentation of xylose, lactose and sucrose results in yellow colonies, sometimes together with medium yellowing
around colonies. Lysine decarboxylation produces a purple colouration around the colonies.
Composition formula is included in SR EN ISO 6579 standard "Horizontal method for the detection of Salmonella
spp.".
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