RELATED MEDIA
Trypticase soy yeast extract medium
Trypticase Soy Broth (BD 211768)................................30.0 g
Yeast extract.................................................................. 3.0 g
Agar..............................................................................15.0 g
Distilled water ad 1000 ml. Adjust for final pH 7.0 - 7.2. Autoclave at 121°C for 15 minutes.
Tryptose agar
Tryptose ......................................................................... 10g
Meat extract....................................................................... 5g
Yeast extract...................................................................... 5g
Proteose peptone ........................................................ 10g
Sodium chloride ................................................................ 5g
Agar................................................................................. 20g
H2O ad 1000 ml, Final pH 7.2 at 25°C
May be supplemented with
L-Cysteine HCl ...........................................................0.25g/l
Trypticase soy yeast extract medium
Trypticase Soy Broth (BD 211768)................................30.0 g
Yeast extract.................................................................. 3.0 g
Agar..............................................................................15.0 g
Distilled water ad 1000 ml. Adjust for final pH 7.0 - 7.2. Autoclave at 121°C for 15 minutes.
Tryptose agar
Tryptose ......................................................................... 10g
Meat extract....................................................................... 5g
Yeast extract...................................................................... 5g
Proteose peptone ........................................................ 10g
Sodium chloride ................................................................ 5g
Agar................................................................................. 20g
H2O ad 1000 ml, Final pH 7.2 at 25°C
May be supplemented with
L-Cysteine HCl ...........................................................0.25g/l
QUALITY CONTROL
Following germs should have a good growth at 35 °C in 24-48 hours: Staphylococcus aureus ATCC 25923,
Streptococcus pyogenes ATCC 19615
Following germs should have a good growth at 35 °C in 24-48 hours: Staphylococcus aureus ATCC 25923,
Streptococcus pyogenes ATCC 19615
COMPOSITION
Pancreatic digest of casein ..................................................................15g
Enzymatic digest of soya bean .............................................................10g
NaCl .......................................................................................................5g
Agar .....................................................................................................15g
Distilled water ad1000ml; adjust pH to 7.3 ± 0.2 Sterilize by autoclaving at
121 ºC for 15 minutes.
Medium may also be used as a blood agar base. Melt, cool to 45-50 ºC
then add 5% sheep or bovine blood.
Pancreatic digest of casein ..................................................................15g
Enzymatic digest of soya bean .............................................................10g
NaCl .......................................................................................................5g
Agar .....................................................................................................15g
Distilled water ad1000ml; adjust pH to 7.3 ± 0.2 Sterilize by autoclaving at
121 ºC for 15 minutes.
Medium may also be used as a blood agar base. Melt, cool to 45-50 ºC
then add 5% sheep or bovine blood.
DESCRIPTION
Medium used for the growth of a large variety of microorganisms. May also be used for colony counting.
Medium used for the growth of a large variety of microorganisms. May also be used for colony counting.
| Tryptone Soya Agar (Casein soya bean digest agar) |
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