OTHER MEDIA
Rogosa selective Lactobacillus agar (composition per liter)
Agar .................................................................................15 g
Sodium acetate............................................................. 15.0 g
Glucose .........................................................................10.0 g
Casein (digested) ..........................................................10.0 g
K2HPO4 ............................................................................6.0 g
Yeast extract .................................................................. 5.0 g
Arabinose ....................................................................... 5.0 g
Saccharose .................................................................... 5.0 g
Ammonium citrate ........................................................... 2.0 g
Sorbitan monooleate ...................................................... 1.0 g
MgSO4.7H2O ................................................................. 0.57 g
MnSO4.7H2O ..................................................................0.12 g
FeSO4.H2O .................................................................... 0.03 g
Acetic acid (glacial) ...................................................... 1.32 ml
Streptococcus equinus & Streptococcus gallolyticus selective medium.
Rogosa selective Lactobacillus agar (composition per liter)
Agar .................................................................................15 g
Sodium acetate............................................................. 15.0 g
Glucose .........................................................................10.0 g
Casein (digested) ..........................................................10.0 g
K2HPO4 ............................................................................6.0 g
Yeast extract .................................................................. 5.0 g
Arabinose ....................................................................... 5.0 g
Saccharose .................................................................... 5.0 g
Ammonium citrate ........................................................... 2.0 g
Sorbitan monooleate ...................................................... 1.0 g
MgSO4.7H2O ................................................................. 0.57 g
MnSO4.7H2O ..................................................................0.12 g
FeSO4.H2O .................................................................... 0.03 g
Acetic acid (glacial) ...................................................... 1.32 ml
Streptococcus equinus & Streptococcus gallolyticus selective medium.
QUALITY CONTROL
Incubate 3 days at 35 °C or 5 days at 30 °C, better in CO2 enriched atmosphere.
Lactobacillus gasseri ATCC 19992: good growth; yellow colonies.
Escherichia coli ATCC 25922: no growth.
Bacillus cereus ATCC 11778: no grow.
Incubate 3 days at 35 °C or 5 days at 30 °C, better in CO2 enriched atmosphere.
Lactobacillus gasseri ATCC 19992: good growth; yellow colonies.
Escherichia coli ATCC 25922: no growth.
Bacillus cereus ATCC 11778: no grow.
COMPOSITION
Peptone.............................................................................. 10g
Meat extract.......................................................................... 8g
Yeast extract........................................................................ 4g
Glucose.............................................................................. 20g
Sodium acetate 3H2O........................................................... 5g
Tween 80............................................................................. 1g
Dipotassium hydrogen phosphate........................................ 2g
Triammonium citrate............................................................. 2g
Magnesium sulfate 7H2O................................................... 0.2g
Manganese sulphate 4H2O.............................................. 0.05g
Agar................................................................................... 10g
H2O ad 1000 ml, Final pH 6.2 at 25°C.
Sterilize by autoclaving at 118 ºC for 15 minutes. Aspect: medium to dark amber coloured, clear to slightly opalescent.
Peptone.............................................................................. 10g
Meat extract.......................................................................... 8g
Yeast extract........................................................................ 4g
Glucose.............................................................................. 20g
Sodium acetate 3H2O........................................................... 5g
Tween 80............................................................................. 1g
Dipotassium hydrogen phosphate........................................ 2g
Triammonium citrate............................................................. 2g
Magnesium sulfate 7H2O................................................... 0.2g
Manganese sulphate 4H2O.............................................. 0.05g
Agar................................................................................... 10g
H2O ad 1000 ml, Final pH 6.2 at 25°C.
Sterilize by autoclaving at 118 ºC for 15 minutes. Aspect: medium to dark amber coloured, clear to slightly opalescent.
DESCRIPTION
A special medium containing growth factors for lactic acid bacteria (Lactobacillus spp. and related germs) from dairy
products, foods, feces and other sources.
A special medium containing growth factors for lactic acid bacteria (Lactobacillus spp. and related germs) from dairy
products, foods, feces and other sources.
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