Following germs should have a good to very good growth at 35-37 °C in 24
hours: Escherichia coli ATCC 25922 and Escherichia coli ATCC 11775.
Contains per litre 10g tryptone (Difco), 5g yeast extract, 5g sodium chloride.
Supplements, such as 1% sorbitol, are added to Luria Bertani agar medium for
Erwinia amylovora and related species.
Adjust the pH of the solution to 7.4. For solid medium add 15 g agar to 1 litre of
Luria Bertani broth.
Sterilize by autoclaving at 121 ºC for 15 minutes.
Medium used for cultivation of Enterobacteriaceae (Escherichia coli) and other organisms. It is nutritionally rich, suitable for the
growth of pure cultures like recombinant strains and for molecular genetic studies.
(c) Costin Stoica