QUALITY CONTROL
Following germs should have a good to very good growth at 35-37
°C in 24 hours: Escherichia coli (25922) and Escherichia coli (11775).
COMPOSITION
Contains per litre 10g tryptone (Difco), 5g yeast extract, 5g sodium
chloride. Supplements, such as 1% sorbitol, are added to Luria
Bertani agar medium for Erwinia amylovora and related species.
Adjust the pH of the solution to 7.4. For solid medium add 15 g agar
to 1 litre of Luria Bertani broth.
Sterilize by autoclaving at 121 ºC for 15 minutes.
DESCRIPTION
Medium used for cultivation of Enterobacteriaceae (Escherichia coli) and other organisms. It is nutritionally rich,
suitable for the growth of pure cultures like recombinant strains and for molecular genetic studies.
(c) Costin Stoica