Adjust pH to 7.3 - 7.7. Do not autoclave. Heat to boil 15 minutes on water bath. Distribute in Petri dishes.
READING
Lactose-positive bacteria (Escherichia coli) form yellow, opaque colonies. Lactose-negative bacteria form
transparent, blue-green colonies (Salmonella, Shigella), sometimes with black center (Proteus).
BILE PREPARATION - harvest ox bile, wait 24 hours for sedimentation. Distribute the upper phase in trays and dry
at 60 ºC. Triturate in a mortar the dryed product. A 8-10% solution obtained from dry bile is equivalent with fresh
bile.
REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de
microbiologie clinica ed. III, 2009, 1045-1084.
READING
Lactose-positive bacteria (Escherichia coli) form yellow, opaque colonies. Lactose-negative bacteria form
transparent, blue-green colonies (Salmonella, Shigella), sometimes with black center (Proteus).
BILE PREPARATION - harvest ox bile, wait 24 hours for sedimentation. Distribute the upper phase in trays and dry
at 60 ºC. Triturate in a mortar the dryed product. A 8-10% solution obtained from dry bile is equivalent with fresh
bile.
REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat de
microbiologie clinica ed. III, 2009, 1045-1084.
COMPOSITION
Lactose ........................................................................................ 1.5 g
Dry ox bile..................................................................................... 0.8 g
Sodium citrate .............................................................................. 0.8 g
Na hiposulfite ............................................................................... 0.8 g
Fe citrate ..................................................................................... 0.2 g
Bromthymole blue 0.2% solution................................................... 4.0 ml
Agar ........................................................................................... 12.0 g
Distilled water ad 1000ml.
Lactose ........................................................................................ 1.5 g
Dry ox bile..................................................................................... 0.8 g
Sodium citrate .............................................................................. 0.8 g
Na hiposulfite ............................................................................... 0.8 g
Fe citrate ..................................................................................... 0.2 g
Bromthymole blue 0.2% solution................................................... 4.0 ml
Agar ........................................................................................... 12.0 g
Distilled water ad 1000ml.
DESCRIPTION
Selective - differential medium for Enterobacteriaceae isolation. Favorize Gram-negative bacteria and inhibits
Gram - positives. Inhibits the swarming of Proteus.
Selective - differential medium for Enterobacteriaceae isolation. Favorize Gram-negative bacteria and inhibits
Gram - positives. Inhibits the swarming of Proteus.
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