Thallium acetate may be added to a max concentration of 1/10,000;
addition of pig serum to a final concentration of 20%; pH adjustment to 7.4
Composition of Hank's balanced salt solution (modified)
2 stock solutions needed:
Stock A
NaCl ............................................................................. 80 g
KCl ................................................................................. 4 g
MgSO4 7 H20 ................................................................. 1 g
MgCl2 6 H2O ................................................................... 1 g
dissolve in 400 ml water CaCl2 (anhydrous) 1.4 g; add water to 500 ml
Stock B
Na2HPO4 12 H2O ........................................................ 1.5 g dissolved in 400 ml water
KH2PO4 ....................................................................... 0.6 g
water ad 500 ml
For use, add 25 ml of stock A to 400 ml of water, followed by 25 ml of stock B; then water ad 500 ml.
addition of pig serum to a final concentration of 20%; pH adjustment to 7.4
Composition of Hank's balanced salt solution (modified)
2 stock solutions needed:
Stock A
NaCl ............................................................................. 80 g
KCl ................................................................................. 4 g
MgSO4 7 H20 ................................................................. 1 g
MgCl2 6 H2O ................................................................... 1 g
dissolve in 400 ml water CaCl2 (anhydrous) 1.4 g; add water to 500 ml
Stock B
Na2HPO4 12 H2O ........................................................ 1.5 g dissolved in 400 ml water
KH2PO4 ....................................................................... 0.6 g
water ad 500 ml
For use, add 25 ml of stock A to 400 ml of water, followed by 25 ml of stock B; then water ad 500 ml.
COMPOSITION
Hank's balanced salt solution (modified) ....................... 500 ml
Water ............................................................................ 750 ml
Bacto brain heart infusin (Difco) .................................... 8.2 g
Bacto PPLO broth w/o CV (Difco) .................................. 8.7 g
Autoclaving at 121 'C for 2-5 minutes.
Yeast extract ................................................................... 60 ml
0.5% phenol red solution ............................................... 4.5 ml
Bacitracin ..................................................................... 250 mg
Meticillin ....................................................................... 250 mg
Hank's balanced salt solution (modified) ....................... 500 ml
Water ............................................................................ 750 ml
Bacto brain heart infusin (Difco) .................................... 8.2 g
Bacto PPLO broth w/o CV (Difco) .................................. 8.7 g
Autoclaving at 121 'C for 2-5 minutes.
Yeast extract ................................................................... 60 ml
0.5% phenol red solution ............................................... 4.5 ml
Bacitracin ..................................................................... 250 mg
Meticillin ....................................................................... 250 mg
DESCRIPTION
Medium used for Mycoplasma hyopneumoniae and M. flocculare isolation.
Medium used for Mycoplasma hyopneumoniae and M. flocculare isolation.
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