PROCEDURE
Disperse culture or pathologic material on the medium surface, then incubate 2-3 days at 37 ºC .
RESULTS
Bordetella colonies are smooth, raised, shiny, 1-2 mm in diameter, sometimes with a gray-black center or
surrounded by hemolysis zone.
REFERENCES
Disperse culture or pathologic material on the medium surface, then incubate 2-3 days at 37 ºC .
RESULTS
Bordetella colonies are smooth, raised, shiny, 1-2 mm in diameter, sometimes with a gray-black center or
surrounded by hemolysis zone.
REFERENCES
- Handbook Culture Media Merck, 1982.
- Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat
de microbiologie clinica ed. III, 2009, 1045-1084.
COMPOSITION
Potatoes infusion ……………………………………………………..… 4.5 g
NaCl ………………………………………………………………….…... 5.5 g
Agar-agar ………………………………………………………………….15 g
Glycerol …………………………………………………….……………. 10 ml
Blood (sheep, rabbit or human) ………………………………150 – 200 ml
Penicillin ………………………………………………………….…….. 250 IU
Distilled water ad 1000 ml, pH 6.6 ± 0.2, autoclave before adding blood, pour in plates. Plates can be stored
refrigerated for 2 weeks.
Addition of 1% peptone (5 g/l peptone from casein & 5 g/l peptone from meat) without penicillin improves the
growth of Bordetella in large vaccine cultures.
Potatoes infusion preparation: wash and peel the potatoes then slice them in small pieces. Add the NaCl and
potatoes in 500 ml water. Boil 30 minutes. Filter then adjust pH to 7.0 .
Potatoes infusion ……………………………………………………..… 4.5 g
NaCl ………………………………………………………………….…... 5.5 g
Agar-agar ………………………………………………………………….15 g
Glycerol …………………………………………………….……………. 10 ml
Blood (sheep, rabbit or human) ………………………………150 – 200 ml
Penicillin ………………………………………………………….…….. 250 IU
Distilled water ad 1000 ml, pH 6.6 ± 0.2, autoclave before adding blood, pour in plates. Plates can be stored
refrigerated for 2 weeks.
Addition of 1% peptone (5 g/l peptone from casein & 5 g/l peptone from meat) without penicillin improves the
growth of Bordetella in large vaccine cultures.
Potatoes infusion preparation: wash and peel the potatoes then slice them in small pieces. Add the NaCl and
potatoes in 500 ml water. Boil 30 minutes. Filter then adjust pH to 7.0 .
DESCRIPTION
Medium was designed for the isolation of Bordetella pertusis, B. parapertussis and other Bordetella species.
Penicillin addition inhibits the growth of associate Gram-positive microflora.
Medium was designed for the isolation of Bordetella pertusis, B. parapertussis and other Bordetella species.
Penicillin addition inhibits the growth of associate Gram-positive microflora.
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