Add glycine, potassium tellurite, egg-yolk emulsion & sulfamethazine after autoclaving and cooling to 45-50 ºC.
Pour in plates. Can be stored 1 month at 4 ºC.
Egg-yolk solution preparation: harvest sterile yolks (see lecithinase test) and mix 3:7 (volumes) with physiological
saline solution.
PROCEDURE
Inoculate medium by spreading the strain on surface and incubate 24-48 hours at 37 ºC.
RESULTS
Staphylococcus forms black shiny, convex colonies, 1-5 mm in diameter, with a narrow white edge, surrounded by a
2-5 mm clear zone. After 48 hours may appear opaque rings within the clear zone.
Micrococci form very small, brown to black colonies, no clear zones
Bacillus species form dark brown colonies, clear zones sometimes appear after 48 hours.
Yeasts form white colonies & no clear zones.
REFERENCES
Pour in plates. Can be stored 1 month at 4 ºC.
Egg-yolk solution preparation: harvest sterile yolks (see lecithinase test) and mix 3:7 (volumes) with physiological
saline solution.
PROCEDURE
Inoculate medium by spreading the strain on surface and incubate 24-48 hours at 37 ºC.
RESULTS
Staphylococcus forms black shiny, convex colonies, 1-5 mm in diameter, with a narrow white edge, surrounded by a
2-5 mm clear zone. After 48 hours may appear opaque rings within the clear zone.
Micrococci form very small, brown to black colonies, no clear zones
Bacillus species form dark brown colonies, clear zones sometimes appear after 48 hours.
Yeasts form white colonies & no clear zones.
REFERENCES
- Helgomar Raducanescu, Valeria Bica-Popii: Bacteriologie veterinara. Ed. Ceres, 326, 1986.
- Alexandru Rafila: Medii de cultura, medii de transport si conservare. In Dumitru Buiuc, Marian Negut: Tratat
de microbiologie clinica ed. III, 2009, 1045-1084. - Handbook Culture Media Merck, 1982.
COMPOSITION
Peptone from casein ……………………………….....................….. 10.0 g
Meat extract …………………………………………........................…. 5.0g
Yeast extract ………………………………………......................……. 1.0 g
Sodium pyruvate …………………………………....................…….. 10.0 g
Glycine ……………………………………………......................……. 12.0 g
Lithium chloride ………………………………….......................……... 5.0 g
Agar-agar …………………………………………....................…….. 15.0 g
Egg-yolk emulsion ……………………………….....................……… 50 ml
Potassium tellurite ………………………………..................……… 0.105 g
Distilled water ad 1000 ml, pH 6.8 ± 0.2
Optional (for Proteus inhibition):
Sulfamethazine …………………………………....................………. 0.05 g
Peptone from casein ……………………………….....................….. 10.0 g
Meat extract …………………………………………........................…. 5.0g
Yeast extract ………………………………………......................……. 1.0 g
Sodium pyruvate …………………………………....................…….. 10.0 g
Glycine ……………………………………………......................……. 12.0 g
Lithium chloride ………………………………….......................……... 5.0 g
Agar-agar …………………………………………....................…….. 15.0 g
Egg-yolk emulsion ……………………………….....................……… 50 ml
Potassium tellurite ………………………………..................……… 0.105 g
Distilled water ad 1000 ml, pH 6.8 ± 0.2
Optional (for Proteus inhibition):
Sulfamethazine …………………………………....................………. 0.05 g
DESCRIPTION
The medium contains pyruvate and glycine which stimulate the growth of staphylococci; also contains lithium chloride
and tellurite which inhibit the growth of other germs. Used for detection from foods and pharmaceutical products.
Staphylococus colonies show two characterisitic features when grown on this medium:
1. forming characteristic zones and rings due to lipolysis (egg-yolk content) and proteolysis
2. producing a black color because of the tellurite.
The medium contains pyruvate and glycine which stimulate the growth of staphylococci; also contains lithium chloride
and tellurite which inhibit the growth of other germs. Used for detection from foods and pharmaceutical products.
Staphylococus colonies show two characterisitic features when grown on this medium:
1. forming characteristic zones and rings due to lipolysis (egg-yolk content) and proteolysis
2. producing a black color because of the tellurite.
| Baird-Parker Staphylococcus agar |
| Egg-yolk emulsion |
(c) Costin Stoica
| Antibiogram |
| Encyclopedia |
| Culture media |
| Biochemical tests |
| Stainings |
| Images |
| Movies |
| Articles |
| Identification |
| Software |
| R E G N U M PROKARYOTAE |
