Biochemical Tests
(c) Costin Stoica
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Mannitol fermentation by Staphylococcus aureus,
negative (left) & positive (right)
Methylene blue decomposition test
positive (left) & negative (right)
Hydrogen sulfide production testing by lead acetate
impreganted paper positive (left) & negative (right)
Litmus milk test
negative (left) & positive (right)
E. coli positive Litmus milk test: pink discoloration,
gas production & curd disruption by gas
Arcanobacterium pyogenes positive Litmus milk test:
pink discoloration, curd production & no gas production
Coagulase test (Stahpylococcus aureus)
both tubes are positive
Liquefaction of clotted horse serum.
Arcanobacterium pyogenes
proteolytic activity,
R E G N U M
PROKARYOTAE
CAMP test, lines 2 & 4 from left to right are positive
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ONPG test (beta-galactosidase)
positive (left) and negative (right)