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(c) Costin Stoica
R E G N U M
PROKARYOTAE
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Bacterial Cultural Characteristics
Actinobacillus pleuropneumoniae  
dependent of NAD-producing
Staphylococcus aureus (yellow line)
Acinetobacter baumannii colonies
on Sheep Blood Agar
Aerococcus viridans colonies on
Sheep Blood Agar
Agar pitting colonies (Bacillus spp.)
Bacillus anthracis colonies on
Sheep Blood Agar
Bacillus anthracis culture on solid
and liquid media
Bacillus subtilis culture on solid and
liquid media
Bacillus licheniformis on Sheep
Blood Agar
Bacillus megaterium colonies
Bacillus mycoides specific colony
shape
Bacillus pumilus on Sheep Blood
Agar
Bacillus thuringiensis on Sheep
Blood Agar
Bacteriophage - infected culture
(spots & plaques no/partial growth)
Bordetella bronchiseptica on solid
medium
Campylobacter jejuni
Clostridium spp. on sulphite agar
Clostridium spp. gas production &
turbidity in Liver Broth medium  
(positive-left and negative - right)
Corynebacterium amycolatum
colonies on Sheep Blood Agar
Dendritiform and galaxy-shape
colonies of
Bacillus spp. Mixed
culture from animal feed
Enterobacter cloacae colonies on
Sheep Blood Agar
Enterococcus faecium on Bile
Esculin Agar (dark brown halos)
Enterococcus faecium colonies on
Sheep Blood Agar
Erysipelothrix rhusiopathiae
colonies after 48h incubation
Erysipelothrix rhusiopathiae after 1
week anaerobic incubation
Escherichia coli beta haemolysis
on Sheep Blood Agar
Escherichia coli on GEAM / Levine
medium (metallic look)
Escherichia coli on Rambach agar
(blue-green colonies)
Fusobacterium necrophorum
in Thioglycollate Broth
Granulicatella / Abiotrophia  
adiacens
colonies, 24h-incubation
Helicobacter pylori colonies on
Sheep Blood Agar
Hydrogen sulfide produced by
Salmonella on XLD agar (black)
Klebsiella pneumoniae (mucous,
confluent colonies)
Kocuria varians colonies on
Sheep Blood Agar
Lactobacillus fermentum
colonies on MRS Agar
Lactococcus lactis subsp. lactis on
Sheep Blood Agar
Lactose-negative colonies on CLED
medium
Lactose-negative colonies on
Istrati-Meitert medium
Lactose-positive colonies on Mac
Conkey medium
Lactose-positive colonies on CLED
medium
Lysinibacillus sphaericus on Sheep
Blood Agar
MacConkey agar: lactose+ (up),
lactose- (left) and lactose- but red
pigmented
Serratia colonies (right)
Micrococcus spp. on solid medium
Moraxella lacunata colonies on
Sheep Blood Agar
Mycobacterium spp. colonies on
Sheep Blood Agar
Mycoplasma agalactiae colonies
(B&W)
Mycoplasma agalactiae colonies,
magnification x70
Mycoplasma agalactiae broth culture
(positive - left and negative - right)
Nocardia asteroides - surface
growth only
Paenibacillus macerans spreading
colonies on Sheep Blood Agar
Pasteurella (Mannheimia)
haemolytica
colonies
Pasteurella multocida colonies on
Sheep Blood Agar
Pasteurella multocida rising and
twisting deposit  when shaked
Proteus mirabilis swarming
Proteus mirabilis swarming and
Dienes effect
Pseudomonas aeruginosa, green,
fluorescent, diffusible pigment
Pseudomonas aeruginosa
haemolysis on Sheep Blood Agar
Pseudomonas aeruginosa on Milk
Agar
Salmonella spp. lactose-negative
colonies on Mac Conkey agar
Salmonella Gallinarum on MSRV
medium (non-motile)
Salmonella Enteritidis motility on
MSRV medium
Serratia marcescens red-pigmented
colonies after 72h incubation
Serratia rubidaea on milk agar
(prodigiosin pigment production)
Staphylococcus colony outskirts on
Sheep Blood Agar. Hyperzoom 200x
Staphylococcus aureus carotenoid
pigment production after 48h
Staphylococcus aureus
alpha- & beta-hemolysis
Staphylococcus chromogenes
colonies on Sheep Blood Agar
Staphylococcus haemolyticus
colonies on Sheep Blood Agar
Staphylococcus xylosus colonies on
Sheep Blood Agar
Streptococci haemolysis: alpha
haemolytic  (right),  beta hemolytic
(left), non-hemolytic (down)
Streptococcus agalactiae on Sheep
Blood Agar (alpha-hemolytic)
Streptococcus agalactiae on Sheep
Blood Agar (beta-hemolytic)
Streptococcus constellatus  colonies
on Sheep Blood Agar
Streptococcus mitis colonies on
Sheep Blood Agar
Streptococcus mutans colonies on
BHI Agar
Streptococcus pyogenes colonies
after 48h incubation
Streptococcus uberis colonies on
Sheep Blood Agar