Other media
Campylobacter selective agar
Peptone protein mixture .................................................... 21 g/l
Electrolyte ........................................................................... 5 g/l
Starch, soluble .................................................................... 1 g/l
Agar-Agar .......................................................................... 13 g/l
Corynebacterium agar
Casein peptone, tryptic digest ......................................... 10.0 g
Yeast extract ..................................................................... 5.0 g
Glucose ............................................................................. 5.0 g
NaCl .................................................................................. 5.0 g
Agar ................................................................................ 15.0 g
Distilled water ad 1000.0 ml, adjust pH to 7.2 - 7.4.
Haemophilus medium
Distilled water ...............................................................  900.0 ml
PPLO agar (Difco B412) .................................................. 34.0 g  
or Mycoplasma Agar Base (Oxoid CM 401) ................... 35.0 g
Glucose ............................................................................. 1.0 g
Sterilize at 121 °C for 15 minutes, cool to 45-50 °C and add:
Horse serum (inactivated) ................................................ 50.0 ml
Yeast extract fresh ......................................................... 100.0 ml
Suspend ingredients in distilled water and bring to the boil to dissolve completely. Adjust to pH 7.4 and add agar;
sterilize, cool to 45 - 50 °C and add:
L-Cysteine HCl ................................................................... 0.5 g/l
Vitamin K1 ........................................................................ 1.0 ml/l
Heterotrophic medium for Hydrogenomonas
KH2PO4 ..............................................................................0.2 g
MgSO4 ...............................................................................0.1 g
Sodium citrate . 2H2O ........................................................0.5 g
Sodium acetate . 3H2O ......................................................0.3 g
Sodium succinate . 6H2O ...................................................2.0 g
Sodium glutamate...............................................................1.0 g
Agar...................................................................................15.0 g
Tryptose...............................................................................5.0 g
Yeast extract........................................................................1.0 g
Corn starch...........................................................................2.0 g
Distilled water.......................................................................1.0 l
Adjust pH to 6.8-7.2. Autoclave 15 minutes at 121C.
ABM (Antarctic bacterial medium) Reddy et al., 2002, 2003
Peptone................................................................................ 5 g
Yeast extract ........................................................................ 2 g
Agar ....................................................................................15 g
Distilled water ad 1000 ml. Adjust pH to 6.9. Used for psychrophilic pseudomonads isolation.
Nutrient Seawater Agar
3ml of glycerol, 1 g of yeast extract, 3 g of peptone, and 15 g of agar per liter of 75% seawater.
Medium is used for marine Vibrio isolation.
Czapek - Dox agar
Sucrose ...............................................................................30.00 g
NaNO3 ..................................................................................3.00 g
MgSO4 x 7 H2O.....................................................................0.50 g
KCl........................................................................................ 0.50 g
FeSO4 x 7 H2O .....................................................................0.01 g
K2HPO4 ................................................................................1.00 g
Agar ....................................................................................13.00 g
Distilled water 1000.00 ml; adjust pH to 7.2.
PPLO broth base
Beef heart infusion ....................................................... 250 g/l
Sodium chloride .............................................................. 5 g/l
Peptic digest of animal tissue .........................................10 g/l
Crystal violet ............................................................... 0.01 g/l
Final pH 7.8 For Mycoplasma isolation add 2.8 ml/l of 1% Potassium Tellurite solution and 25% Ascitic fluid .
TY broth

Casein enzymatic hydrolysate type I ......................8g
Yeast extract powder .............................................5g
NaCl ........................................................,,,,,,,.......5g
H2O ad 1,000 ml
(c) Costin Stoica
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Bordet-Gengou Medium
Bordet-Gengou Agar Base (BD 248200)…………………….30.0 g
Glycerol………………………………………………….………10.0 ml
Proteose peptone …….....……………………………….……..10.0 g
H2O  ......…………………………………….……………………840 ml
Adjust pH to 6.7 +/- 0.2. Autoclave at 121º C. Cool  to 45-50ºC and add 150 ml sterile defibrinated rabbit blood. Used
for Bordetella pertussis & Pseudomonas pertucinogena cultivation.
BCSA medium
NaCl : 5g Saccharose : 10g Lactose : 10g Phennol red: 0,08g Crystal violet : 0,002g Trypticase peptone : 10g Yeast
extract : 1,5g  Agar : 14g  Sulfate de polymyxine B : 600 000 UI Gentamicine : 0,01 Vancomycine : 0,025 H2O ad
1000 ml. Used for Burkholderia cepacia isolation.
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