| V-Factor Requirement |
DESCRIPTION
V-factor or nicotinamide adenine dinucleotide (NAD) is a coenzyme involved in redox reactions. Some bacteria
cannot produce NAD and need to get it from the substrate or grow as a satellite of a NAD-producing organism.
V-factor or nicotinamide adenine dinucleotide (NAD) is a coenzyme involved in redox reactions. Some bacteria
cannot produce NAD and need to get it from the substrate or grow as a satellite of a NAD-producing organism.
Satellitism can be demonstrated on an agar medium lacking the V-factor
by cross-inoculating the plate with a feeder strain (e.g. Staphylococcus or
Pseudomonas). The NAD-requiring strain will grow only in the vicinity of
the NAD-producing strain.
V-factor impregnated disks may be used instead of the feeder strain.
NOTES:
NAD is destroyed by autoclaving the medium.
The satellite phenomenon may be difficult to achieve in ordinary agar
plates because blood may contain various amounts of V-factor. For a
NAD-free medium autoclaving is required after adding the blood.
by cross-inoculating the plate with a feeder strain (e.g. Staphylococcus or
Pseudomonas). The NAD-requiring strain will grow only in the vicinity of
the NAD-producing strain.
V-factor impregnated disks may be used instead of the feeder strain.
NOTES:
NAD is destroyed by autoclaving the medium.
The satellite phenomenon may be difficult to achieve in ordinary agar
plates because blood may contain various amounts of V-factor. For a
NAD-free medium autoclaving is required after adding the blood.
REFERENCES:
1. H. Raducanescu, V.Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. J. G.Holt et al., 1994.Begey’s manual of Determinative Bacteriology, 9-edition, Williams & Wilkins.
1. H. Raducanescu, V.Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. J. G.Holt et al., 1994.Begey’s manual of Determinative Bacteriology, 9-edition, Williams & Wilkins.
| Actinobacillus pleuropneumoniae dependent of V factor (NAD)-producing Staphylococcus (line) and CAMP positive reaction |
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