| Cellulose Hydrolysis |
DESCRIPTION
Some bacteria are able to hydrolyze carboxymethylcellulose. The cellulases produced by bacteria will lower the
viscosity of the medium (a thick syrup of cellulose in a minimal medium has a high viscosity).
Some bacteria are able to hydrolyze carboxymethylcellulose. The cellulases produced by bacteria will lower the
viscosity of the medium (a thick syrup of cellulose in a minimal medium has a high viscosity).
MEDIUM PREPARATION
Na-taurocholate ............................................................................... 5 g
NH4H2PO4 ......................................................................................... 1 g
MgSO4 * 7H2O .................................................................................. 0.2 g
KCl ................................................................................................... 0.2 g
Bromthymolblue ............................................................................... 0.05 g
CaCO3 ............................................................................................. 3 g
Carboxymethylcellulose .................................................................... 5 g
PROCEDURE
Harvest a well isolated colony and inoculate a medium tube. Incubate at
37 °C, min. 24 hours. Positive control: Erwinia chrysanthemi, negative
control: Erwinia carotovora subsp. carotovora
Na-taurocholate ............................................................................... 5 g
NH4H2PO4 ......................................................................................... 1 g
MgSO4 * 7H2O .................................................................................. 0.2 g
KCl ................................................................................................... 0.2 g
Bromthymolblue ............................................................................... 0.05 g
CaCO3 ............................................................................................. 3 g
Carboxymethylcellulose .................................................................... 5 g
PROCEDURE
Harvest a well isolated colony and inoculate a medium tube. Incubate at
37 °C, min. 24 hours. Positive control: Erwinia chrysanthemi, negative
control: Erwinia carotovora subsp. carotovora
RESULTS
Check viscosity by comparioson with a negative control. Reaction is positive if the inoculated medium is more fluid
than the control tube.
Check culture for the yellowish-green color produced by the cellulose degradation. The color change is not
obligatory.
Check viscosity by comparioson with a negative control. Reaction is positive if the inoculated medium is more fluid
than the control tube.
Check culture for the yellowish-green color produced by the cellulose degradation. The color change is not
obligatory.
(c) Costin Stoica
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