| MIU (Motility Indole Urea) |
DESCRIPTION
Test checks simultaneously for the production of indole, urea hydrolysis and bacteria motility. Medium, together with
TSI and Simmons agar, is most used for Enterobacteriaceae identification, especially Escherichia and Salmonella.
Test checks simultaneously for the production of indole, urea hydrolysis and bacteria motility. Medium, together with
TSI and Simmons agar, is most used for Enterobacteriaceae identification, especially Escherichia and Salmonella.
MEDIUM PREPARATION
Composition:
MIU base
Proteose peptone Difco ..................................................10g
Beef extract ......................................................................5g
NaCl ................................................................................ 5g
KH2PO4 ......................................................................... 2g
Phenol red solution 1/500 .............................................. 6 ml
Agar ................................................................................3g
Distilled water ................................................................. 900ml
Adjust pH to 6.8-6.9
Urea - glucose solution
10 g urea + 1 g glucose + distilled water ad 100 ml.
Preparation: to 90 ml base medium add 10 ml urea-glucose solution.
PROCEDURE
Harvest a well isolated colony and inoculate a MIU tube by stabbing the
medium. Incubate at 37 °C, 24 hours.
RESULTS
Motility is positive if the entire medium became opaque (semisolid state of
the medium permits the bacteria moving). Result is negative if the culture
grows only on the stabbing line.
For the indole test add 2-3 drops of Ehrlich-Covacs reactive. A red layer on
the surface of the medium appears if the reaction is positive.
Urea is hydrolysed if the entire medium turns red.
Composition:
MIU base
Proteose peptone Difco ..................................................10g
Beef extract ......................................................................5g
NaCl ................................................................................ 5g
KH2PO4 ......................................................................... 2g
Phenol red solution 1/500 .............................................. 6 ml
Agar ................................................................................3g
Distilled water ................................................................. 900ml
Adjust pH to 6.8-6.9
Urea - glucose solution
10 g urea + 1 g glucose + distilled water ad 100 ml.
Preparation: to 90 ml base medium add 10 ml urea-glucose solution.
PROCEDURE
Harvest a well isolated colony and inoculate a MIU tube by stabbing the
medium. Incubate at 37 °C, 24 hours.
RESULTS
Motility is positive if the entire medium became opaque (semisolid state of
the medium permits the bacteria moving). Result is negative if the culture
grows only on the stabbing line.
For the indole test add 2-3 drops of Ehrlich-Covacs reactive. A red layer on
the surface of the medium appears if the reaction is positive.
Urea is hydrolysed if the entire medium turns red.
NOTES
Melt base medium by boiling it and wait to cool under 50 ºC before adding Urea - glucose solution, otherwise urea
will be inactivated. Final medium state must be semisolid.
Melt base medium by boiling it and wait to cool under 50 ºC before adding Urea - glucose solution, otherwise urea
will be inactivated. Final medium state must be semisolid.
REFERENCES:
1. Helgomar Raducanescu, Valeria.Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
1. Helgomar Raducanescu, Valeria.Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
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A demonstration of TSI, MIU and Simmons utilization is available in video section.
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