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Firmicutes => Bacilli => Lactobacillales => Enterococcaceae => Enterococcus => Enterococcus haemoperoxidus Svec et al. 2001
(decompose hydrogen peroxide into oxygen and water on blood-agar media)
Lancefield group D.
Gram-positive, elongated cocci, grouped in pairs, short chains or small groups.
Non-motile.
Colonies are circular and smooth with entire margins, 1 mm in diameter after 24 h of
cultivation and up to 3-4 mm after 5 days. A slightly yellowish pigment may be
produced. No hemolysis on sheep-blood agar.
Uniform turbidity produced in BHI broth culture at 37 °C.
Optimum temperature 37 °C, grow at 10 °C but not at 45°C. Facultatively anaerobic.
Growth enhanced slightly by cultivation in a 5% CO
2 atmosphere. Grow in 6.5% NaCl.
Isolated from non-potable water.
Unknown.
  1. Pavel Svec and  Luc A. Devriese, 2009: Genus I. Enterococcus (ex Thiercelin and Jouhaud 1903) Schleifer and Kilpper-Bälz
    1984, 32VP in Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume Three, Vos, P.D.; Garrity, G.; Jones, D.;
    Krieg, N.R.; Ludwig, W.; Rainey, F.A.; Schleifer, K.-H.; Whitman, W.B. (Eds.), pp 594-606.
  2. P Svec, L A Devriese, I Sedl√°cek, M Baele, M Vancanneyt, F Haesebrouck, J Swings, and J Doskar. Enterococcus
    haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water. Int J Syst Evol Microbiol July 2001 51:
    1567-74.
Catalase reaction is positive when cultivated on blood agar and negative when is
cultivated on media without blood.
Positive results for  acetoin production, hippurate hydrolysis, aesculin hydrolysis, pyrrolidonyl arylamidase, leucine
arylamidase, arginine dihydrolase, acid production from: glycerol, ribose, galactose, D-glucose, D-fructose, D-mannose, methyl
alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, sucrose, trehalose, melezitose,
beta-gentiobiose & maltotriose.

Negative results for  beta-glucuronidase, beta-galactosidase, alkaline phosphatase, urease, acid production from: erythritol, D- or
L-arabinose, D- or L-xylose, adonitol, methyl beta-xyloside, L-sorbose, rhamnose, dulcitol, inositol, sorbitol, methyl
alpha-D-mannoside, melibiose, inulin, D-raffinose, xylitol, D-lyxose, D-tagatose, D- or L-fucose, D- or L-arabitol, gluconate, 2- &
5-ketogluconate.

Variable results for acid production from: starch, glycogen, D-turanose & mannitol.
Enterococcus haemoperoxidus
(c) Costin Stoica
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