Staphylococcus haemolyticus on Sheep blood agar
Staphylococcus haemolyticus
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Staphylococcaceae, Genus Staphylococcus, Staphylococcus haemolyticus  
Schleifer and Kloos 1975.
Gram-positive cocci, nonmotile, nonsporing, 0.8–1.3 μm in diameter, grouped in pairs
and tetrads.
Colonies are nonpigmented or yellowish, smooth, glistening, opaque, with entire
margins, 5-9 mm in diameter and haemolytic. Hemolysis may be weak sometimes.  
Facultatively anaerobic,  grow better aerobically. Temperature range  18 to 40 ºC
(optimum 34-35 ºC). Used media: Trypticase Soy Agar + 5% sheep blood, Mueller
Hinton agar, P agar. Grows well in medium containing 10% NaCl; variable and poor
growth at 15% NaCl.
Isolated from human & animal skin. Susceptible to novobiocin & lysostaphin. Resistant to lysozyme.
Commensal of the skin, rarely cause infections in immunocompromised  organisms (septicemia, peritonitis, otitis, and urinary tract
infections). Known for its resistance to many antibiotics. Has occasionally been associated with mastitis in cattle.
  1. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Wiliams S.T., 1994. Bergey's Manual of Determinative Bacteriology, Ninth Edition,
    Williams & Wilkins, Baltimore. Group 17, Gram-Positive Cocci, 527-558.
  2. Schleifer K.H. & Kloos W.E.: Isolation and characterization of staphylococci from human skin. Amended descriptions of
    Staphylococcus epidermidis and Staphylococcus saprophyticus and descriptions of three new species: Staphylococcus cohnii,
    Staphylococcus haemolyticus, and Staphylococcus xylosus. International Journal of Systematic Bacteriology, 1975, 25, 50-61.
  3. Karl-Heinz Schleifer and Julia A. Bell, 2009. Family VIII. Staphylococcaceae fam. nov.. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R.
    Krieg, W. Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The
    Firmicutes, Springer, 392-426.
Positive results for arginine dihydrolase, catalase, acid production from glucose,
glycerol, maltose,sucrose & trehalose.

Negative results for alkaline phosphatase, urease, oxidase, coagulase-rabbit
plasma, clumping factor, heat-stable nuclease, beta-galactosidase, acid production
from mannose, xylitol, raffinose, xylose, arabinose, cellobiose, erythritol, erythrose,
fucose,
gentiobiose, salicin & melezitose.

Variable results for acetoin production, beta-glucosidase, beta-glucuronidase,
deoxyribonuclease, nitrate reduction, acid production from: lactose, mannitol,
fructose, turanose & ribose.
(c) Costin Stoica
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