| Endospore Stainig Schaeffer & Fulton 1933 |
DESCRIPTION
Endospores are non-reproductive structures produced by some Gram-positive bacteria, being a form of resistance
in unfavorable conditions, unlike the eukaryotic spores wich have a reproductive role. Bacterial endospores exists
in a dormant state waiting for reactivation when conditions become favorable.
Endospores are non-reproductive structures produced by some Gram-positive bacteria, being a form of resistance
in unfavorable conditions, unlike the eukaryotic spores wich have a reproductive role. Bacterial endospores exists
in a dormant state waiting for reactivation when conditions become favorable.
Staining technique consists in dyeing the smear with malachite green. Only the endospore
retain the stain. Vegetative cell is decolorized when washed with water. The counterstain
safranin is then used to color vegetative cell.
REAGENTS
Malachite green solution 5%,
Safranin solution 0.5% or diluted fuchsin.
PROCEDURE
Dry and heat-fix the slide then cover it with Malachite green solution. Heat the slide for 3
minutes; avoid boiling. Wash in running tap water.
Cover the slide with Safranin solution and wait 1 minute. Remove solution, wash and dry
the slide.
retain the stain. Vegetative cell is decolorized when washed with water. The counterstain
safranin is then used to color vegetative cell.
REAGENTS
Malachite green solution 5%,
Safranin solution 0.5% or diluted fuchsin.
PROCEDURE
Dry and heat-fix the slide then cover it with Malachite green solution. Heat the slide for 3
minutes; avoid boiling. Wash in running tap water.
Cover the slide with Safranin solution and wait 1 minute. Remove solution, wash and dry
the slide.
| Malachite green stained, central, non-deforming spores (Bacillus anthracis) |
RESULTS
Light-green inclusions (endospores) in pink-to-red vegetative cells.
Light-green inclusions (endospores) in pink-to-red vegetative cells.
A demonstration of staining procedure is available in video section.
(c) Costin Stoica
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