Methylene Blue Stainig
DESCRIPTION
A stainig method for bacteria visualisation that cannot differentiate between bacteria.


REAGENTS
Loffler methylene blue solution: methylene blue crystals 1 g, phenol crystals melted,
96% alcohol 10 ml, distilled water 100 ml.


PROCEDURE
Dry and heat fix the slide, then cover it with methylene blue solution.
Wait 2 to 5 minutes then rinse in distilled water and dry the slide.


RESULTS
Vegetative forms of bacteria  appear blue colored.


NOTES:
Using old, oxidized Loffler methylene blue solution (stored over few months) capsule
will be pink-colored and the rest of the cell will be blue.


REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Dumitru Buiuc, Carmen Panzaru: Coloratii, coloranti si reactivi pentru microscopie,
micrometrie. In Dumitru Buiuc, Marian Negut: Tratat de microbiologie clinica ed. III,
2009, 1173-1215.
(c) Costin Stoica
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Methylene blue crystals
Methylene blue stained cells (Bacillus megaterium)