| Methylene Blue Stainig |
DESCRIPTION
Stainig method that cannot differentiate between bacteria.
Stainig method that cannot differentiate between bacteria.
REAGENTS
Loffler methylene blue solution: methylene blue crystals 1 g, phenol crystals
melted, 96% alcohol 10 ml, distilled water 100 ml.
PROCEDURE
Dry and heat fix the slide, then cover it with methylene blue solution
Wait 2 to 5 minutes then rinse in distilled water and dry the slide.
RESULTS
Vegetative forms of bacteria appear blue colored.
Loffler methylene blue solution: methylene blue crystals 1 g, phenol crystals
melted, 96% alcohol 10 ml, distilled water 100 ml.
PROCEDURE
Dry and heat fix the slide, then cover it with methylene blue solution
Wait 2 to 5 minutes then rinse in distilled water and dry the slide.
RESULTS
Vegetative forms of bacteria appear blue colored.
NOTES:
Using old, oxidized, Loffler methylene blue solution (stored over few months) capsule will be pink-colored and the
rest of the cell will be blue.
REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Dumitru Buiuc, Carmen Panzaru: Coloratii, coloranti si reactivi pentru microscopie, micrometrie. In Dumitru Buiuc,
Marian Negut: Tratat de microbiologie clinica ed. III, 2009, 1173-1215.
Using old, oxidized, Loffler methylene blue solution (stored over few months) capsule will be pink-colored and the
rest of the cell will be blue.
REFERENCES
1. H. Raducanescu, V. Bica-Popii,1986. Bacteriologie veterinara, Ed. Ceres, Bucuresti.
2. Dumitru Buiuc, Carmen Panzaru: Coloratii, coloranti si reactivi pentru microscopie, micrometrie. In Dumitru Buiuc,
Marian Negut: Tratat de microbiologie clinica ed. III, 2009, 1173-1215.
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