Brevundimonas (Pseudomonas) vesicularis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas vesicularis
(Busing, Doll & Freitag 1953) Galarneault & Leifson 1964. Moved to Class Alphaproteobacteria, Order  
Caulobacterales, Family Caulobacteraceae, Genus Brevundimonas,
Brevundimonas vesicularis (Büsing et al. 1953) Segers et al. 1994.
Old synonyms:
Corynebacterium vesiculare  Büsing et al. 1953, Pseudomonas vesiculare  (Büsing et al. 1953) Galarneault and Leifson
Gram-negative rods with a single polar flagellum, 0.5 by 1-4 μm.
Accumulate poli-beta-hydroxybutyrate.
Yellow / orange colonies (non fluorescent, non diffusible, carotenoid pigment
Obligately aerobic, optimum temperature 26-30
ºC (no growth at 4 or 41 ºC). Can
grow on Nutrient agar
and Nutrient broth.
First isolated from the seminal vesicle of  Hirudo medicinalis (medicinal leech). Other isolates from water, soil, slime deposits in
paper mills, rarely clinical human specimens.
Unknown. Few reports of bacteremia in immunocompromised patients.
  1. George M. Garrity, Julia A. Bell and Timothy Lilburn: Order V. Caulobacterales Henrici and Johnson 1935b, 4AL. In Bergey’s
    Manual of Systematic Bacteriology Second Edition Volume Two The Proteobacteria Part C The Alpha-, Beta-, Delta-, and
    Epsilonproteobacteria, George M. Garrity Editor-in-Chief, 2005, pp 285-323.
  2. Bobbak Vahid: Brevundimonas Vesicularis Bacteremia Following Allogeneic Bone Marrow Transplantation. The Internet Journal of
    Infectious Diseases. 2006. Volume 5 Number 1.
  3. Busing K.H., Doll W. & Freitag K.: Die Bakterienflora der medizinischen Blutegel. Archiv fur Mikrobiologie, 1953, 19, 52-86.
  4. Galarneault T.P. & Leifson E.: Pseudomonas vesiculare (Busing et al.) nov. comb. International Bulletin of Bacteriological
    Nomenclature and Taxonomy, 1964, 14, 165-168.
Can oxidize isopropanol to acetone.
Positive results for alpha-glucosidase, esculin hydrolysis, alkaline and acid phosphates, ester lipase (C8), leucine arylamidase,
phosphoamidase, trypsin & oxidase.
Can utilize for growth: glucose, galactose, maltose, cellobiose, acetate, butyrate, succinate, L-malate, D&L-alanine, L-aspartate, DL-

Negative results for arginine dihydrolase, chymotrypsin, nitrates and nitrites reduction, gelatin liquefication, indole formation, urease,
lysine and ornithine decarboxylases, phenylalanine deaminase, lecithinase(egg yolk), and lipase (Tween 80), lipase (C14), cystine
arylamidase, alpha-fucosidase, alpha- and beta-galactosidase, beta-glucosidase, N-acetyl beta-glucosidase, alpha-mannosidase.
Cannot utilize: D-malate, tyrosine, L-valine, n-valerate, fructose, gluconate, lactose, trehalose, xylose, L-histidine, pantothenate,
glycerol, erythritol, D- and L-arabinose, D-ribose, D- and L-xylose, adonitol, methyl-P-xyloside, D-fructose, D-mannose, L-sorbose,
dulcitol, inositol, mannitol, sorbitol, methyl-alpha-D-mannoside, methyl-alpha-D-glucose, N-acetylglucosamine, amygdalin, arbutin,
salicin, lactose, D-melibiose, sucrose, trehalose, inulin, D-melezitose, D-raffinose, glycogen, xylitol, P-gentiobiose, D-turanose, D-
lyxose, D-tagatose, D-
and L-fucose, D- and L-arabitol, gluconate, 2-ketogluconate, 5-ketogluconate, propionate, isobutyrate,
heptanoate, n-caproate, caprylate, pelargonate, caprate, oxalate, malonate, maleate, glutarate, adipate, pimelate, suberate, azelate,
sebacate, glycolate, D-malate, DL-glycerate, D-tartrate, L-tartrate, meso-tartrate, levulinate, citraconate, itaconate, mesaconate, citrate,
phenylacetate, benzoate, o-hydroxybenzoate, m-hydroxybenzoate, p-hydroxybenzoate, D- and L-mandelate, phthalate, isophthalate,
terephthalate, glycine, L-norleucine, L-cysteine, L-methionine, L-phenylalanine, D- and L-tryptophan, trigonelline, L-ornithine, L-lysine,
L-citrulline, DL-kynurenine, betaine, creatine, beta-alanine, urea, acetamide, sarcosine, ethylamine, butylamine, amylamine,
ethanolamine, benzylamine, diaminobutane, spermine, histamine, tryptamine & glucosamine.

Variable results for utilization of acetate, fumarate & lactate.
Valine arylamidase is present in some strains, but not the type strain.
(c) Costin Stoica
Culture media
Biochemical tests
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