Pseudomonas oleovorans (P. pseudoalcaligenes)
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,  
Pseudomonas oleovorans
Lee and Chandler 1941 emend. Saha et al. 2010.
Pseudomonas oleovorans subsp. lubricantis Saha et al. 2010, subsp. nov
Pseudomonas oleovorans subsp. oleovorans Lee and Chandler 1941, subsp. nov.

Synonym:
Pseudomonas pseudoalcaligenes  Stanier 1966. According to Saha et al. 2010, Pseudomonas pseudoalcaligenes Stanier
1966 is a later heterotypic synonym of  
Pseudomonas oleovorans Lee and Chandler.
Gram negative, 0.7-0.8 / 1.2-2.5 µm, motile rods.
Strictly aerobic, optimal temperature 35 ºC, poor growth at 41 ºC.
Nutrient agar / nutrient broth.
Isolated from soil, water & clinical samples (sputum, urine, sinus discharge, cerebrospinal fluid) - rarely. Pseudomonas oleovorans
is isolated from oil-water emulsions used as lubricants and cooling agents in the cutting and grinding of metals. Apparently the
organism lives on some normal constituent of the cutting compound, probably the naphthenic acids, which act as emulsifying agents.
Opportunistic pathogen. Possible cause of  meningitis or pneumonia (doubtful – admin note).
  1. Stanier R.Y.. In: Stanier R.Y., Palleroni N.J. & Doudoroff M.: The aerobic pseudomonads: a taxonomic study. Journal of General
    Microbiology, 1966, 43, 159-271.
  2. Saha R., Sproer C., Beck B. & Bagley S.: Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of
    Pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of Pseudomonas oleovorans ATCC 8062T. Curr. Microbiol.,
    2010, 60, 294-300.
  3. W.A. Cowlishaw, Margaret E. Hughes & H.C.R. Simpson Meningitis caused by an alkali-producing pseudomonad. J. clin. Path.,
    1976, 29, 1088-1090.
  4. Huertas MJ, Luque-Almagro VM, Martinez-Luque M, Blasco R, Moreno-Vivian C, Castillo F, Roldan MD. (2006) Cyanide
    metabolism of Pseudomonas pseudoalcaligenes CECT5344: role of siderophores. Biochemical Society Transactions 34(Pt 1):
    152-5.
  5. S.F. Nishino and J.C. Spain: Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes. Appl Environ Microbiol. 1993
    August; 59(8): 2520-2525.
  6. P.D. Fiorella and J.C. Spain: Transformation of 2,4,6-Trinitrotoluene by Pseudomonas pseudoalcaligenes JS52. Appl. Environ.
    Microbiol., May 1997, 2007-2015, Vol 63, No. 5.
  7. Yoon-Dong Park, Hana Yi, Keun Sik Baik, Chi Nam Seong, Kyung Sook Bae, Eun Young Moon, and Jongsik Chun: Pseudomonas
    segetis sp. nov., isolated from soil. Int J Syst Evol Microbiol November 2006 56:2593-2595.
Fructose is the only carbohydrate utilized. Can utilize glutarate, D-malate,
mesaconate, ethanol, DL-arginine. Oxidase positive.
Mannitol, gluconate, 2-ketogluconate are not utilized. Alkaline & acid phosphatase,
egg-yolk reaction, H
2S production, indole production, urease, lysine decarboxylase,
ornithine decarboxylase, starch hydrolysis & lipase are  negative. Gelatin liquefaction
is variable

Can use free cyanide or cyano-metal complexes as nitrogen source. Can use
nitrobenzene as the sole source of carbon. Can degrade 2,4,6-trinitrotoluene (TNT).
(c) Costin Stoica
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