Ralstonia pickettii
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Betaproteobacteria, Order  Burkholderiales, Family Burkholderiaceae, Genus Ralstonia,
Ralstonia pickettii  Yabuuchi et al. 1996.
Historical synonyms:
Pseudomonas pickettii, Ralston et al. 1973, Burkholderia pickettii Yabuuchi et al. 1993.

3 biovars: Va-1, Va-2, and biovar 3 (
Pseudomonas thomasii  /  Ralstonia mannitolilytica *).
*The original name mannitolytica (sic), has been corrected to mannitolilytica.
Gram negative rods. Sometimes motile by a single polar flagellum.
Strictly aerobic, optimal temperature 35 ºC, variable growth at 42 ºC.
Grow on media: Trypticase Soy Agar  ± 5% sheep blood, Mueller-Hinton agar, Mac
Conkey agar
Isolated from soil, water, rarely from human clinical samples (pleural & ascitic fluid, pus,  biopsy samples, blood samples).
Can cause pneumonia, meningitis, endocarditis, and osteomyelitis, bacteremia, nosocomial infections, particularly in
immunocompromised patients (reports of biovar Va-1 acute meningitis, biovar Va-2 bacteremia &
P. thomasii hospital-acquired
colonization and infection).
  1. Ralston E., Palleroni N.J. & Dodoroff M.: Pseudomonas pickettii, a new species of clinical origin related to Pseudomonas
    solanacearum. International Journal of Systematic Bacteriology, 1973, 23, 15-19.
  2. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T. & Arakawa M.: Proposal of Burkholderia gen. nov. and
    transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia
    cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol., 1992, 36, 1251-1275.
  3. Yabuuchi E., Kosako Y., Yano I., Hotta H., & Nishiuchi Y.: Transfer of two Burkholderia and an Alcaligenes species to Ralstonia
    gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith
    1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol. Immunol., 1995, 39, 897-904.
  4. Mark R. Bruins, Sanjay Kapil and Frederick W. Oehme: Pseudomonas pickettii: A Common Soil and Groundwater Aerobic Bacteria
    with Pathogenic and Biodegradation Properties. Ecotoxicology and Environmental Safety Volume 47, Issue 2, October 2000,
    Pages 105-111.
  5. Ryan MP, Pembroke JT, Adley CC: Ralstonia pickettii: a persistent gram-negative nosocomial infectious organism. J Hosp Infect.
    2006 Mar;62(3):278-84. Epub 2005 Dec 6.
  6. Thierry De Baere, Sophia Steyaert, Georges Wauters, Paul De Vos,Johan Goris, Tom Coenye, Tetsushi Suyama, Gerda
    Verschraegen & Mario Vaneechoutte: Classification of Ralstonia pickettii biovar 3/‘thomasii’ strains (Pickett 1994) and of new
    isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov. International Journal of Systematic and
    Evolutionary Microbiology (2001), 51, 547–558.
  7. Teizi Urakami, Chieko Ito-Yoshida, Hisaya Araki, Toshio Kijima, Ken-Ichiro Suzuki, and Kazuo Komagata: Transfer of
    Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and Description of Burkholderia vandii
    sp. nov. Int J Syst Bacteriol April 1994 44:235-245.
Can degrade chlorophenols, aromatic hydrocarbons, 2,4-dichlorophenoxyacetic acid
and pentacyclic triterpeniod compounds.
Negative for H
2S production, indole production. See tables for more.
(c) Costin Stoica
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Fluorescent
diffusible pigments
Poli-beta-hydroxybutyrate
inclusions
Growth at
41 ºC
Arginine
dihydrolase
Oxidase
Denitrification
Gelatin
hydrolysis
Starch
hydrolysis
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Lactose
Maltose
Mannitol
Nitrates reduction
Tartrate
Maleate
Va-1
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Va-2
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P. thomasii /
R. mannitolilytica
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Glucose
utilization
Trehalose
utilization
2-Ketogluconate
utilization
m-Inositol
utilization
Lecithinase
Urease
Esculin
hydrolysis
ONPG
Citrate
utilization
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Differentiating biovars