Pseudomonas gessardii
Arginine dihydrolase, oxidase & catalase positive. Levan is produced from sucrose.
Can produce esterases C5, C6, C8, C9 and C10.

Starch and o-nitrophenyl beta-D-galactopyranoside cannot be assimilated.
Does not form indole from tryptophan. No H
2S production in TSI. Does not decarboxylate lysine or ornithine.
No elastase, coagulase, beta-xylosidase (p-nitrophenyl beta-D-xylopyranoside test) or phenylalanine deaminase activities are
reported. No activity against DNA. Fibrinolysin test is negative. Voges-Proskauer test and gelatin liquefaction are negative.

Positive for the utilization of D-glucose, D-fructose, D-trehalose, D-ribose, L-arabitol, xylitol, glycerol, myo-inositol, D-mannitol,
adonitol, meso-erythritol, L-malate, cis-aconitate, citrate, 2-keto-D-gluconate, N-acetyl D-glucosamine, D-gluconate, protocatechuate,
quinate, betaine, putrescine, DL-alpha-amino-n-butyrate, adipate, DL-lactate, caprate, caprylate, succinate, fumarate, glutarate, DL-
glycerate, D-glucosamine, DL-beta-hydroxybutyrate, L-aspartate, L-glutamate, L-proline, D-alanine, L-alanine, L-serine, malonate,
propionate, L-tyrosine & 2-oxoglutarate.

Negative for alpha-D-galactosidase, beta-D-galactosidase, phospho-beta-D-galactosidase, alpha-L-arabinosidase, alpha-D-
glucosidase, beta-D-glucosidase, beta-D-galacturonohydrolase, beta-D-glucuronidase, alpha-maltosidase, beta-maltosidase, N-
acetyl-alpha-o-glucosaminidase, alpha-L-fucosidase, beta-D-fucosidase, beta-L-fucosidase, beta-D-lactosidase, alpha-D-
mannosidase, beta-D-mannosidase, alpha-D-xylosidase, beta-D-xylosidase, esterase-C 18, N-benzoyl-leucine arylamidase, N-
benzoyl-L-alanine-4-methoxy arylamidase, N-carboxybenzoxy-arginyl-4-methoxy arylamidase, N-acetyl-glycyl-L-lysine arylamidase.

Not utilized : L-sorbose, D-melibiose, sucrose, D-raffinose, maltotriose, maltose, alpha-lactose, lactulose, 1 -O-methyl beta-
galactopyranoside, 1-O-methyl alpha-galactopyranoside, D-cellobiose, beta-gentiobiose, 1-0-methyl beta-D-glucopyranoside,
aesculin, L-arabinose, D-xylose, palatinose, alpha-L-rhamnose, alpha-L-fucose, D-melezitose, dulcitol, D-tagatose, maltitol, D-
turanose, D-sorbitol, hydroxyquinoline beta-glucuronide, D-lyxose, 1 –O methyl alpha-D-glicopyranoside, 3-O-methyl D-
glucopyranose, D-saccharate, L-tartrate, D-tartrate, meso-tartrate, tricarballylate, D-glucuronate, D-galacturonate, 5-keto-D-gluconate,
L-tryptophan, phenylacetate, 3-phenylpropionate, m-coumarate, trigonelline, histamine & L-histidine.

Variable reactions for urease, denitrification, lecithinase, assimilation of mucate, itaconate, benzoate, ethanolamine, D-galactose & D-
Cultural characteristics
Biochemical characters
Gram-negative rods, motile by one polar flagellum.
Pyoverdin is produced on King B medium and on S1 medium. No phenazine
pigments are observed on King A medium. Non-haemolytic. Strictly aerobic, growth
temperature 4-35 ºC. No growth at 41 ºC. Media: Nutritive agar, Trypticase Soy Agar,
MacConkey agar, and King’s medium. Significant growth is observed only at low NaCl
concentrations (up to 0.8 %) and not at 5 or 7 %.
Isolated from mineral waters.
Unknown (none).
  1. Verhille S., Baida N., Dabboussi F., Hamze M., Izard D. & Leclerc H.: Pseudomonas gessardii sp. nov. and Pseudomonas migulae
    sp. nov., two new species isolated from natural mineral waters. Int. J. Syst. Bacteriol., 1999, 49, 1559-1572.
  2. Elena P. Ivanova, Nataliya M. Gorshkova, Tomoo Sawabe, Karin Hayashi, Nataliya I Kalinovskaya, Anatolii M. Lysenko, Natalie V.
    Zhukova, Dan V. Nicolau, Tatyana A. Kuznetsova, Valery V. Mikhailov, and Richard Christen: Pseudomonas extremorientalis sp.
    nov., isolated from a drinking water reservoir. Int J Syst Evol Microbiol November 2002 52:2113-20.
  3. Van Den Beld, M. J., Reinders, E., Notermans, D. W. and Reubsaet, F. A. 2016. Possible misidentification of species in the
    Pseudomonas fluorescens lineage as Burkholderia pseudomallei and Francisella tularensis, and emended descriptions of
    Pseudomonas brenneri, Pseudomonas gessardii and Pseudomonas proteolytica. Int. J. Syst. Evol. Microbiol., 66, 3420-3425.
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas gessardii
Verhille et al. 1999
(c) Costin Stoica
Culture media
Biochemical tests
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