Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas extremorientalis Ivanova et al. 2002.
Gram-negative, 0.8-1 x 2.0-3.5 μm, motile rods, with a single polar flagellum. Non-
spore-forming. Does not accumulate poli-beta-hydroxybutyrate as intracellular carbon
Colonies are hemolytic, round, 3-6 mm, circular, regular, convex, smooth. Fluorescent
pigment produced. Strictly aerobic. Growth temperature 4-35 ºC, optimal 25 ºC. No
growth at 42 ºC. Grows on Nutritive agar, Trypticase Soy Agar, Mueller-Hinton agar,
King’s A and B media. Tolerates up to 6% NaCl in the culture medium. Grows at pH
6.0-10.0, optimally at pH 7.0.
Isolated from a drinking water reservoir near Vladivostok City, Russia.
Susceptible to gentamicin, kanamycin, streptomycin and tetracycline. Resistant to ampicillin, benzylpenicillin, carbenicillin,
lincomycin and polymyxin.
- Elena P. Ivanova, Nataliya M. Gorshkova, Tomoo Sawabe, Karin Hayashi, Nataliya I Kalinovskaya, Anatolii M. Lysenko, Natalie V.
Zhukova, Dan V. Nicolau, Tatyana A. Kuznetsova, Valery V. Mikhailov, and Richard Christen: Pseudomonas extremorientalis sp.
nov., isolated from a drinking water reservoir. Int J Syst Evol Microbiol November 2002 52:2113-20.
Positive results for arginine dihydrolase, caseinase, catalase, lecithinase nitrate reduction & oxidase.
Oxidizes dextrin, glycogen, Tween 40, Tween 80, N-acetyl-D-glucosamine, adonitol, L-arabinose, D-arabitol, cellobiose, D-fructose,
D-galactose, alpha-lactose, lactulose, maltose, D-mannitol, D-mannose, D-methyl glucoside, sorbitol, sucrose, D-trehalose,
tyranose, xylitol, methyl pyruvate, mono-methyl succinate, acetic acid, cisaconitic acid, citric acid, formic acid, D-galactonic acid
D-galacturonic acid, D-gluconic acid, D-glucosaminic acid, D-glucuronic acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid,
gamma-hydroxybutyric acid, itaconic acid, 2-oxobutyric acid, 2-oxoglutaric acid, 2-oxovaleric acid, DL-lactic acid, malonic acid,
propionic acid, quinic acid, D-saccharic acid, sebacic acid, succinic acid, bromosuccinic acid, succinamic acid, glucuronamide,
alaninamide, D-alanine, L-alanine, L-alanyl-glycine, L-asparagine, L-aspartic acid, L-glutamic acid, L-histidine, L-leucine, L-proline,
L-pyroglutamic acid, D-serine, L-serine, L-threonine, DL-camitine, caminobutyric acid, urocanic acid, inosine, uridine, thymidine,
2-aminoethanol, glycerol, alpha-DL-glycerol phosphate, glucose 1-phosphate.
Negative results for chitin hydrolysis, indole production, lipase, gelatinase, amylase, starch hydrolysis & Voges-Proskauer test.
No utilization of: D-glucose, melibiose, lactose, adonitol, L-rhamnose, L-fucose, myoinositol, alpha-cyclodextrin, gentiobiose,
N-acetyl-D-galactosamine, i-erythritol, D-raffinose, alpha-hydroxybutyric acid, p-hydroxyphenylacetic acid, hydroxy-L-proline,
L-ornithine, L-phenylalanine, phenylethylamine, putrescine & 2,3-butanediol.
(c) Costin Stoica