Pseudomonas  extremorientalis
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas extremorientalis
Ivanova et al. 2002.
Gram-negative, 0.8-1 x 2.0-3.5 μm, motile rods, with a single polar flagellum. Non-
spore-forming. Does not accumulate poli-beta-hydroxybutyrate as intracellular carbon
reserve.
Colonies are hemolytic, round, 3-6 mm, circular, regular, convex, smooth. Fluorescent
pigment produced. Strictly aerobic. Growth temperature 4-35
ºC, optimal 25 ºC. No
growth at 42 ºC. Grows on Nutritive agar, Trypticase Soy Agar, Mueller-Hinton agar,
King’s A and B media. Tolerates up to 6% NaCl in the culture medium. Grows at pH
6.0-10.0, optimally at pH 7.0.
Isolated from a drinking water reservoir near Vladivostok City, Russia.
Susceptible to gentamicin, kanamycin, streptomycin and tetracycline. Resistant to ampicillin, benzylpenicillin, carbenicillin,
lincomycin and polymyxin.
Unknown (none).
  1. Elena P. Ivanova, Nataliya M. Gorshkova, Tomoo Sawabe, Karin Hayashi, Nataliya I Kalinovskaya, Anatolii M. Lysenko, Natalie V.
    Zhukova, Dan V. Nicolau, Tatyana A. Kuznetsova, Valery V. Mikhailov, and Richard Christen: Pseudomonas extremorientalis sp.
    nov., isolated from a drinking water reservoir. Int J Syst Evol Microbiol November 2002 52:2113-20.
Positive results for arginine dihydrolase, caseinase, catalase, lecithinase nitrate reduction & oxidase.
Oxidizes dextrin, glycogen, Tween 40, Tween 80, N-acetyl-D-glucosamine, adonitol, L-arabinose, D-arabitol, cellobiose, D-fructose,
D-galactose, alpha-lactose, lactulose, maltose, D-mannitol, D-mannose, D-methyl glucoside, sorbitol, sucrose, D-trehalose,
tyranose, xylitol, methyl pyruvate, mono-methyl succinate, acetic acid, cisaconitic acid, citric acid, formic acid, D-galactonic acid
D-galacturonic acid, D-gluconic acid, D-glucosaminic acid, D-glucuronic acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid,
gamma-hydroxybutyric acid, itaconic acid, 2-oxobutyric acid, 2-oxoglutaric acid, 2-oxovaleric acid, DL-lactic acid, malonic acid,
propionic acid, quinic acid, D-saccharic acid, sebacic acid, succinic acid, bromosuccinic acid, succinamic acid, glucuronamide,
alaninamide, D-alanine, L-alanine, L-alanyl-glycine, L-asparagine, L-aspartic acid, L-glutamic acid,  L-histidine, L-leucine, L-proline,
L-pyroglutamic acid, D-serine, L-serine, L-threonine, DL-camitine, caminobutyric acid, urocanic acid, inosine, uridine, thymidine,
2-aminoethanol, glycerol, alpha-DL-glycerol phosphate, glucose 1-phosphate.

Negative results for chitin hydrolysis, indole production, lipase, gelatinase, amylase, starch hydrolysis & Voges-Proskauer test.
No utilization of: D-glucose, melibiose, lactose, adonitol, L-rhamnose, L-fucose, myoinositol, alpha-cyclodextrin, gentiobiose,
N-acetyl-D-galactosamine, i-erythritol, D-raffinose, alpha-hydroxybutyric acid, p-hydroxyphenylacetic acid, hydroxy-L-proline,
L-ornithine, L-phenylalanine, phenylethylamine, putrescine & 2,3-butanediol.
(c) Costin Stoica
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