Brevundimonas (Pseudomonas) diminuta
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Bacteria => Proteobacteria => Gammaproteobacteria => Pseudomonadales => Pseudomonadaceae => Pseudomonas =>
Pseudomonas diminuta Leifson and Hugh 1954. Moved to Bacteria => Proteobacteria =>  Alphaproteobacteria => Caulobacterales =>
Caulobacteraceae => Brevundimonas =>
Brevundimonas diminuta (Leifson and Hugh 1954) Segers et al. 1994  (type species of the
genus).
(Synonym:
Bacterium parvulum)
Gram negative, 0.5 x 1.0-4.0 μm, motile rods by one polar flagellum.
Smooth, glistening, opaque, circular, convex with entire edge colonies. No pigment
produced, non-hemolytic.
Strictly aerobic, optimal temperature 30 ºC. Can grow at 41 ºC, not at 4 ºC.
Pantothenate, biotin, cystine and cyanocobalamin are required as growth factors.
Media: Nutritive agar, Trypticase Soy Agar, Mueller-Hinton agar, TSI, Mac Conkey.
Isolated from water & clinical specimens.
Unknown. Is commonly used as a test organism for the efficiency of water filters due to the small size of the bacterium.
  1. Bergey’s Manual of Determinative Bacteriology, 9th ed., 1994.
  2. Leifson E. & Hugh R.: A new type of polar monotrichous flagellation. Journal of General Microbiology, 1954, 10, 68-70.
  3. Kaltenbach C. M., Moss C. W. & Weaver R. E.: Cultural and biochemical characteristics and fatty acid composition of
    Pseudomonas diminuta and Pseudomonas vesiculare. J Clin Microbiol. 1975 April; 1(4): 339–344.
Accumulate poli-β-hydroxybutyrate as intracellular carbon reserve, especially in nitrogen-
deficient media. Denitrification negative, nitrite rarely produced from nitrate.
Tween 80 starch, gelatin, egg yolk reaction negative.

Oxidase and catalase positive. Acetate, butyrate, L-leucine, L-serine, L-histidine, DL-norvaline, and L-aspartate are utilized.

L-rhamnose, mannitol, L-arabinose, sorbitol, sucrose, D-cellobiose, starch, DL-lactate, D-xylose, adonito1, D-mannose, D-glucose,
D-galactose, arbutin, salicin, maltose, trehalose, propionate, amygdalin, isobutyrate n -caproate, adipate, pimelate, suberate, azelate,
sebacate, glycine and aconitate are not utilized
Esculin is not hydrolyzed. Negative for valine arylamidase,  alpha- & beta-glucosidase activities. Indole is not formed.

Utilization of  valerate, succinate, fumarate, L-malate, 2-ketoglutarate, D-alpha-alanine, L-alanine, L-isoleucine, L-valine, L-norleucine,
DL-norvaline, L-threonine, L-tyrosine & L-arginine is variable.
(c) Costin Stoica
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