Does not hydrolyse aesculin and does not produce acid from L-tartrate. Nitrates are not reduced. Levan-sucrase-negative,
non-pectinolytic and does not degrade polypectates at pH 5 or 8. Does not assimilate L-sorbose, alpha-D-melibiose, D-raffiose,
maltotriose, maltose, alpha-lactose, lactulose, methyl 1-O-beta-galactopyranoside, methyl 1-O-alpha-galactopyranoside,
D-cellobiose, beta-gentiobiose, methyl 1-O-beta-D-glucopyranoside, palatinose, alpha-L-rhamnose, alpha-L-fucose, D-melezitose,
dulcitol, D-tagatose, maltitol, hydroxyquinoline-beta-glucuronide, methyl 1-O-alpha-D-glycopyranoside, methyl 3-O-D-glucopyranose,
L-tartrate, tricarballylate, L-tryptophan, phenylacetate, gentisate, m-hydroxybenzoate, 3-phenylpropionate, m-coumarate, trigonelline,
L-histidine & tryptamine.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Bacteria => Proteobacteria => Gammaproteobacteria => Pseudomonadales => Pseudomonadaceae => Pseudomonas =>
Pseudomonas costantinii Munsch et al. 2002. Close to Pseudomonas tolaasii.
Gram-negative straight rods, motile by a single polar flagellum.
Produces a fluorescent diffusible greenish pigment. Colonies may be hemolytic.
Aerobic, optimal growth temperature 28 °C.
Media: Trypticase Soy Agar, Nutrient agar, King’s B agar.
Isolated from cultivated mushroom sporophores (Agaricus bisporus).
Causal agent of brown blotch disease in mushrooms. Does not induce a hypersensitive reaction on tobacco leaves.
- Munsch P., Alatossava T., Marttinen N., Meyer J.M., Christen R. & Gardan L.: Pseudomonas costantinii sp. nov., another causal
agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland. Int. J. Syst. Evol. Microbiol., 2002,
52, 1973-1983.
- Munsch P., Alatossava T.: Several pseudomonads, associated with the cultivated mushrooms Agaricus bisporus or Pleurotus
sp., are hemolytic. Microbiol Res. 2002;157(4):311-5.
Oxidase-positive, arginine-dihydrolase-positive and hydrolyse gelatin. Produces acid
from DL-lactate, D-tartrate, erythritol, mannitol & sorbitol. Utilizes alpha-D-glucose,
beta-D-fructose, D-galactose, D-trehalose, D-mannose, D-ribose, L-arabinose, D-
xylose, D-arabitol, L-arabitol, xylitol, glycerol, myo-inositol, D-mannitol, D-sorbitol,
adonitol, D-lyxose, meso-erythritol, D-saccharate, mucate, meso-tartrate, D-malate,
L-malate, cis-aconitate, trans-aconitate, citrate, D-glucuronate, D-galacturonate, 2-keto-
D-gluconate, 5-keto-D-gluconate, N-acetyl-d-glucosamine, D-gluconate, protocatechuate, p-hydroxybenzoate, quinate, benzoate,
betain, putrescine, DL-alpha-amino-n-butyrate, histamine, DL-lactate, caprate, caprylate, succinate, fumarate, glutarate, DL-glycerate,
DL-alpha-amino-n-valerate, ethanolamine, D-glucosamine, itaconate, DL-beta-hydroxybutyrate, L-aspartate, L-glutamate, L-proline,
D-alanine, L-alanine, L-serine, malonate, propionate, L-tyrosine and 2-oxoglutarate.

(c) Costin Stoica