Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas congelans Behrendt et al. 2003.
Gram-negative, non-sporeforming rods, motile by one polar flagellum, 2.5-2.9 µm
wide and about 0.8 µm long.
Aerobic, optimal growth temperature 21 ºC. Media: Trypticase Soy Agar, Nutrient agar,
King’s agar A & B. Colonies on King A and B media are white-yellowish, smooth with
regular margins and produce a pigment with light yellow-green fluorescence on UV
light. Nonhemolytic on sheep blood agar.
First isolated from the phyllosphere of grasses in Germany. Presents ice nucleation activity.
Possible plant pathogen.
- Behrendt U., Ulrich A. & Schumann P.: Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas
trivialis sp. nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int. J. Syst. Evol. Microbiol., 2003, 53, 1461-
Negative for hydrolysis of starch, formation of levan from sucrose, reduction of nitrate to
nitrite, denitrification, arginine dihydrolase, DNase, indole production, formation of H2S
from sodium thiosulphate, oxidase & utilization of D-tartrate, N-acetyl-D-glucosamine,
DL-carnitine, D-cellobiose, glucose 6-phosphate, glycyl L-aspartic acid, gamma-hydroxybutyric acid, hydroxy-L-proline, glycogen,
p-hydroxyphenylacetic acid, alpha-ketovaleric acid, L-ornithine, putrescine or L-rhamnose.
Positive for gelatinase, casein hydrolysis, catalase, aesculin hydrolysis & aerobic acid formation from glucose.
Can utilize L-arabinose, D-arabitol, m-inositol, D-mannitol, sucrose, D-galacturonic acid, D-glucosaminic acid, D-glucuronic acid,
L-asparagine, L-pyroglutamic acid, L-threonine, D-trehalose,erythritol, glycerol, DL-lactic acid, 2-aminoethanol, i-erythritol,
alpha-D-glucose, glycyl, L-glutamic acid, DL-alpha-glycerol phosphate, alpha-hydroxybutyric acid, beta-hydroxybutyric acid, formic
acid, L-histidine, monomethyl succinate, L-proline, D-psicose, quinic acid, D-sorbitol, D-serine, succinamic acid, & Tween 40.
(c) Costin Stoica