Positive for utilization of citrate, D-galactose, trans-aconitate and mesaconate, utilization of fumarate, glutarate, pyruvate,
4-hydroxybenzoate, malonate, 2-ketogluconate, benzylformate, o-aminobenzoate, hippurate, glycolate & benzylformate, glycogen,
D-glucose, acetic acid, citric acid, formic acid, lactic acid, propionic acid, succinic acid, D-alanine, L-alanine, L-asparagine, L-aspartic
acid, D-xylose, D-galacturonic acid, D-gluconic acid, L-glutamic acid, sebacic acid, phenylethylamine, putrescine and L-proline.

Negative for utilization of trehalose, hydroxy-L-proline, 2-butanol, acetamide, adipate,  mucate, acetate & propionate,
N-acetyl-D-galactosamine, adonitol, L-arabinose, i-erythritol, myo-inositol, xylitol, alpha-D-lactose, D-mannose, D-melibiose,
D-raffinose, L-rhamnose, turanose, D-mannitol, D-sorbitol, D-fructose, L-fucose, D-galactose, gentiobiose, maltose, sucrose,
inosine, uridine & thymidine.
Pseudomonas citronellolis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas citronellolis
Seubert 1960.
Gram-negative, 0.5 x 1-1.5 μm rods, motile with polar single flagella.
Transparent, raised circular colonies with slightly wrinkled edges. Hemolysis is
produced on defibrinated rabbit blood agar.
Obligately aerobic (grow
s anaerobically only in the presence of nitrate), optimal
growth temperature 30 ºC. Can grow at 41 ºC. Uncertain growth at 4 ºC (positive
according to Prakash et al. 2007 and negative according to Elke Lang et. al 2007).

Grows on m
edia: Trypticase Soy Agar, Nutrient agar, LB medium. The species shows
positive reactions for growth in the presence of 5% NaCl.
Isolated from oil sludge, soil.
  1. Seubert W.: Degradation of isoprenoid compounds by microorganisms. I. Isolation and characterization of an isoprenoid-
    degrading bacterium, Pseudomonas citronellolis n. sp. Journal of Bacteriology, 1960, 79, 426-434.
  2. Linda L. Clark, Joseph J. Dajcs, Celeste H. McLean, John G. Bartell and David W. Stroman: Pseudomonas otitidis sp. nov.,
    isolated from patients with otic infections. Int J Syst Evol Microbiol 56 (2006), 709-714; DOI  10.1099/ijs.0.63753-0.
  3. Gupta, Sanjay Kumar, Kumari, Rekha, Prakash, Om, Lal, Rup. Pseudomonas panipatensis sp. nov., isolated from an oil-
    contaminated site. Int J Syst Evol Microbiol 2008 58: 1339-1345.
  4. Elke Lang, Barbara Griese, Cathrin Spröer, Peter Schumann, Maike Steffen, and Susanne Verbarg: Characterization of
    Pseudomonas azelaica’ DSM 9128, leading to emended descriptions of Pseudomonas citronellolis Seubert 1960 (Approved
    Lists 1980) and Pseudomonas nitroreducens Iizuka and Komagata 1964 (Approved Lists 1980), including Pseudomonas
    multiresinivorans as its later heterotypic synonym. Int J Syst Evol Microbiol April 2007 57:878-882.
Can degrade the toxic hydrocarbon constituents present in oily sludge. Citronellol and
related compounds oxidation (use citronellol as sole carbon source).
Arginine dihydrolase, catalase, oxidase, hydrolysis of aesculin, hypoxanthine, acid
production from fructose & glycerol are positive. Nitrates are reduced to nitrites.

Lecithinase, methyl red, hydrolysis of gelatin & casein,  acetylmethylcarbinol, H
2S &
indole negative. No acid & no gas produced from: lactose, galactose, maltose, glucose,
dulcitol, inositol, mannitol, inulin, dextrin, sucrose, arabinose.
(c) Costin Stoica
Culture media
Biochemical tests
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