Oxidize the following substrates: acetic acid, N-acetylglucosamine, alaninamide, alanine, L-alanyl-glycine, gamma-aminobutyric acid,
2-aminoethanol, L-arabinose, D-arabitol, L-asparagine, L-aspartic acid, cis-aconitic acid, bromosuccinic acid, DL-carnitine, citric acid,
formic acid, D-fructose, D-galactonic acid lactone, D-galactose, D-galacturonic acid, alpha-D-glucose, D-gluconic acid,
D-glucosaminic acid, D-glucuronic acid, L-glutamic acid, glycerol, L-histidine, beta-hydroxybutyric acid, gamma-hydroxybutyric acid,
p-hydroxyphenyl acetic acid, hydroxy-L-proline, inosine, meso-inositol, alpha-ketoglutaric acid, DL-lactic acid, malonic acid,
D-mannitol, D-mannose, methylpyruvate, monomethylsuccinate, L-ornithine, L-proline, propionic acid, L-pyroglutamic acid, quinic
acid, D-saccharic acid, sebacic acid, L-serine, D-sorbitol, succinamic acid, succinic acid, sucrose, D-trehalose, L-threonine, Tween
40, Tween 80, uridine and urocanic acid (results obtained with BIOLOG GN microplates).
Oxidation of 2,3-butanediol, dl-a-glycerol phosphate, glycyl L-glutamic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alphaketovaleric acid, L-leucine, maltose, psicose and turanose is variable.
Oxidation of the other organic substrates included in BIOLOG GN microplates is negative.
Assimilation of N-acetyl-d-glucosamine, cis-aconitate, trans-aconitate, DL-alanine,
DL-alpha-amino-n-butyrate, DL-alpha-amino-n-valerate, L-arabinose, D-arabitol, L-aspartate, betaine, caprate, caprylate, citrate,
ethanolamine, beta-D-fructose, fumarate, D-galactose, D-galacturonate, D-gluconate, D-glucosamine, alpha-D-glucose,
D-glucuronate, L-glutamate, glutarate, DL-glycerate, glycerol, 2-keto-D-gluconate, 2-oxoglutarate, DL-beta-hydroxybutyrate,
p-hydroxybenzoate, DL-lactate, D-lyxose, L-malate, D-mannose, D-mannitol, mucate, myo-inositol, L-proline, propionate,
protocatechuate, quinate, D-ribose, D-saccharate, L-serine, D-sorbitol, succinate, sucrose, D-trehalose, trigonelline, L-tyrosine &
D-xylose is positive (results obtained with Biotype 100 strips).
Assimilation of benzoate, D(-)malate, malonate, putrescine & D(-)tartrate is variable.
Other substrates of Biotype 100 strips are not assimilated.
2-aminoethanol, L-arabinose, D-arabitol, L-asparagine, L-aspartic acid, cis-aconitic acid, bromosuccinic acid, DL-carnitine, citric acid,
formic acid, D-fructose, D-galactonic acid lactone, D-galactose, D-galacturonic acid, alpha-D-glucose, D-gluconic acid,
D-glucosaminic acid, D-glucuronic acid, L-glutamic acid, glycerol, L-histidine, beta-hydroxybutyric acid, gamma-hydroxybutyric acid,
p-hydroxyphenyl acetic acid, hydroxy-L-proline, inosine, meso-inositol, alpha-ketoglutaric acid, DL-lactic acid, malonic acid,
D-mannitol, D-mannose, methylpyruvate, monomethylsuccinate, L-ornithine, L-proline, propionic acid, L-pyroglutamic acid, quinic
acid, D-saccharic acid, sebacic acid, L-serine, D-sorbitol, succinamic acid, succinic acid, sucrose, D-trehalose, L-threonine, Tween
40, Tween 80, uridine and urocanic acid (results obtained with BIOLOG GN microplates).
Oxidation of 2,3-butanediol, dl-a-glycerol phosphate, glycyl L-glutamic acid, alpha-hydroxybutyric acid, alpha-ketobutyric acid,
alphaketovaleric acid, L-leucine, maltose, psicose and turanose is variable.
Oxidation of the other organic substrates included in BIOLOG GN microplates is negative.
Assimilation of N-acetyl-d-glucosamine, cis-aconitate, trans-aconitate, DL-alanine,
DL-alpha-amino-n-butyrate, DL-alpha-amino-n-valerate, L-arabinose, D-arabitol, L-aspartate, betaine, caprate, caprylate, citrate,
ethanolamine, beta-D-fructose, fumarate, D-galactose, D-galacturonate, D-gluconate, D-glucosamine, alpha-D-glucose,
D-glucuronate, L-glutamate, glutarate, DL-glycerate, glycerol, 2-keto-D-gluconate, 2-oxoglutarate, DL-beta-hydroxybutyrate,
p-hydroxybenzoate, DL-lactate, D-lyxose, L-malate, D-mannose, D-mannitol, mucate, myo-inositol, L-proline, propionate,
protocatechuate, quinate, D-ribose, D-saccharate, L-serine, D-sorbitol, succinate, sucrose, D-trehalose, trigonelline, L-tyrosine &
D-xylose is positive (results obtained with Biotype 100 strips).
Assimilation of benzoate, D(-)malate, malonate, putrescine & D(-)tartrate is variable.
Other substrates of Biotype 100 strips are not assimilated.
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Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Bacteria => Proteobacteria => Gammaproteobacteria => Pseudomonadales => Pseudomonadaceae => Pseudomonas =>
Pseudomonas brassicacearum Achouak et al. 2000. 2 subspecies:
- brassicacearum Achouak et al. 2000.
- neoaurantiaca Ivanova et al. 2009
Pseudomonas brassicacearum Achouak et al. 2000. 2 subspecies:
- brassicacearum Achouak et al. 2000.
- neoaurantiaca Ivanova et al. 2009
Gram negative rods, 1.0-1.5 μm x 0.5 μm in diameter.
Forms mucoid colonies with regular margins. Brown-orange diffusible pigment & a
fluorescent pigment produced. Strictly aerobic, optimum growth temperature 30 °C.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, King's B medium.
fluorescent pigment produced. Strictly aerobic, optimum growth temperature 30 °C.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, King's B medium.
Isolated from Brassica napus & Arabidopsis thaliana rhizoplane.
Brassica napus root-associated bacteria. Possible pathogenic action on tomato. No hypersensitivity reaction produced on tobacco
leaves.
leaves.
- Achouak W., Sutra L., Heulin T., Meyer J.M., Fromin N., Degraeve S., Christen R. & Gardan L.: Pseudomonas brassicacearum
sp. nov. and Pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from Brassica napus and
Arabidopsis thaliana. Int. J. Syst. Evol. Microbiol., 2000, 50, 9-18. - Ivanova E.P., Christen R., Bizet C., Clermont D., Motreff L., Bouchier C., Zhukova N.V., Crawford R.J. & Kiprianova E.A.:
Pseudomonas brassicacearum subsp. neoaurantiaca subsp. nov., orange-pigmented bacteria isolated from soil and the
rhizosphere of agricultural plants. Int. J. Syst. Evol. Microbiol., 2009, 59, 2476-2481.
Oxidase-positive, arginine dihydrolase-positive, produces levan, not pectinolytic.
Reduction of nitrate to nitrite is variable.
Hydrolyses gelatin, but not aesculin.
Acid produced from sucrose and sorbitol but not from erythritol & mannitol.
Reduction of nitrate to nitrite is variable.
Hydrolyses gelatin, but not aesculin.
Acid produced from sucrose and sorbitol but not from erythritol & mannitol.
(c) Costin Stoica
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