Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas azotifigens Hatayama et al. 2005 (synonym: Pseudomonas azotofixans)
Gram-negative, straight, 2-5 / 0.5 μm, motile rods.
Produce translucent, wrinkled colonies. No pigments are produced on King medium
A or Luisetti medium. Strictly aerobic, growth temperature 28-41 ºC (optimum 30 ºC).
Grows on: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar, Mueller-Hinton agar,
Isolated from a compost pile.
- Hatayama K., Kawai S., Shoun H., Ueda Y. & Nakamura A.: Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium
isolated from a compost pile. Int. J. Syst. Evol. Microbiol., 2005, 55, 1539-1544.
Starch and Tween 80 are hydrolysed. Catalase and oxidase are produced.
Acetic acid, cis-aconitic acid, L-alaninamide, D-alanine, L-alanine, D-arabitol,
L-asparagine, bromosuccinic acid, citric acid, formic acid, D-gluconic acid, alpha-D-glucose, L-glutamic acid, glycogen,
hydroxy-L-proline, alpha-hydroxybutyric acid, beta-hydroxybutyric acid, itaconic acid, alpha-ketobutyric acid, alpha-ketoglutaric acid,
alpha-ketovaleric acid, DL-lactic acid, L-leucine, D-mannitol, methyl pyruvate, monomethyl succinate, L-proline, propionic acid,
D-psicose, L-pyroglutamic acid, D-saccharic acid, sebacic acid, succinamic acid, succinic acid and Tweens 40 and 80 are oxidized.
Nitrate reduction and denitrification are negative.
(c) Costin Stoica