Gelatin liquefaction,arginine dihydrolase are positive. Acid but no gas produced  from
glucose, galactose, fructose, arabinose, xylose, maltose, sucrose, lactose (weak,
after long incubation). Glycerol, D-glucose, D-fructose and gluconate are assimilated.
Methyl pyruvate, cis-aconitic acid, citric acid, formic acid, beta-hydroxybutyric acid,
p-hydroxyphenylacetic acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid,
D-alanine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-proline,
L-serine, DL-carnitine, gamma-aminobutyric acid and urocanic acid  are utilized.
No acid production from raffinose. Lecithinase and indole are not produced and levan is not formed. Nitrates reduction, hydrogen
sulfide, urease, lecithinase, esculin & starch hydrolysis are negative.
Erythritol, D-arabinose, L-xylose, adonitol, methyl beta-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl alpha-
D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, D-cellobiose, D-maltose, D-lactose,
D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose,
D-fucose, L-fucose, L-arabitol, 2-ketogluconate and 5-ketogluconate are not assimilated.
Dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, adonitol, D-cellobiose, erythritol, L-fucose, gentiobiose, myo-inositol, lactose,
lactulose, maltose, D-melibiose, methyl beta-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose,
turanose, xylitol are not utilized.
Pseudomonas asplenii
Taxonomy
Morphology
Growth conditions
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas,
Pseudomonas asplenii
(Phytomonas asplenii Ark and Tompkins 1946) Savulescu 1947.  
Gram negative, 0.3-0.5 x 1.2-2.4 μm, rods. Motile, with 1 to 3 polar flagella.
Colonies on nutrient agar are circular, smooth and 2–3 mm in diameter (after 24 h of
cultivation). Fluorescein is produced on King B medium. Pyocyanine is not produced
on King A medium.Strictly aerobic, optimum growth temperature 26 ºC, minimum 1
ºC, maximum 34 ºC.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar / Nutrient broth, Fermi's,
Cohn's & Uschinsky's solutions
Isolated from soil & plants (ferns - Asplenium nidus).
Fern pathogen - causes bacterial leaf blight of Asplenium nidus.
  1. Ark P.A. & Tompkins, CM (1946). "Bacterial leaf blight of bird's-nest fern". Phytopathology 36: 758-761.
  2. Savulescu T.: Contribution à la classification des bacteriacées phytopathogènes. Analele Academiei Romane Series III Tom 22,
    Memoire, 1947, 4, 1-26.
  3. Tvrzova L., Schumann P., Sproer C., Sedlacek I., Pacova Z., Sedo O., Zdrahal Z., Steffen M. & Lang E.: Pseudomonas moraviensis
    sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds & emended description of
    Pseudomonas asplenii. Int. J. Syst. Evol. Microbiol., 2006, 56, 2657-2663.
(c) Costin Stoica
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