Gelatin liquefaction,arginine dihydrolase are positive. Acid but no gas produced from
glucose, galactose, fructose, arabinose, xylose, maltose, sucrose, lactose (weak,
after long incubation). Glycerol, D-glucose, D-fructose and gluconate are assimilated.
Methyl pyruvate, cis-aconitic acid, citric acid, formic acid, beta-hydroxybutyric acid,
p-hydroxyphenylacetic acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid,
D-alanine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-proline,
L-serine, DL-carnitine, gamma-aminobutyric acid and urocanic acid are utilized.
glucose, galactose, fructose, arabinose, xylose, maltose, sucrose, lactose (weak,
after long incubation). Glycerol, D-glucose, D-fructose and gluconate are assimilated.
Methyl pyruvate, cis-aconitic acid, citric acid, formic acid, beta-hydroxybutyric acid,
p-hydroxyphenylacetic acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid,
D-alanine, L-aspartic acid, L-glutamic acid, L-histidine, hydroxy-L-proline, L-proline,
L-serine, DL-carnitine, gamma-aminobutyric acid and urocanic acid are utilized.
No acid production from raffinose. Lecithinase and indole are not produced and levan is not formed. Nitrates reduction. hydrogen
sulfide, urease, lecithinase, esculin & starch hydrolysis are negative.
Erythritol, D-arabinose, L-xylose, adonitol, methyl b-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl
a-D-mannoside, methyl a-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, D-cellobiose, D-maltose, D-lactose,
D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose,
D-fucose, L-fucose, L-arabitol, 2-ketogluconate and 5-ketogluconate are not assimilated.
Dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, adonitol, D-cellobiose, erythritol, L-fucose, gentiobiose, myo-inositol, lactose,
lactulose, maltose, D-melibiose, methyl b-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose,
turanose, xylitol are not utilized.
sulfide, urease, lecithinase, esculin & starch hydrolysis are negative.
Erythritol, D-arabinose, L-xylose, adonitol, methyl b-xyloside, L-sorbose, L-rhamnose, dulcitol, inositol, D-sorbitol, methyl
a-D-mannoside, methyl a-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, salicin, D-cellobiose, D-maltose, D-lactose,
D-melibiose, D-sucrose, inulin, D-melezitose, D-raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose,
D-fucose, L-fucose, L-arabitol, 2-ketogluconate and 5-ketogluconate are not assimilated.
Dextrin, glycogen, Tween 40, N-acetyl-D-galactosamine, adonitol, D-cellobiose, erythritol, L-fucose, gentiobiose, myo-inositol, lactose,
lactulose, maltose, D-melibiose, methyl b-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose,
turanose, xylitol are not utilized.
|
Taxonomy
Morphology
Growth conditions
Biochemical characters
Ecology
Pathogenicity
References
Bacteria => Proteobacteria => Gammaproteobacteria => Pseudomonadales => Pseudomonadaceae => Pseudomonas =>
Pseudomonas asplenii (Phytomonas asplenii Ark and Tompkins 1946) Savulescu 1947.
Pseudomonas asplenii (Phytomonas asplenii Ark and Tompkins 1946) Savulescu 1947.
Gram negative, 0.3-0.5 x 1.2-2.4 μm, rods. Motile, with 1 to 3 polar flagella.
Colonies on nutrient agar are circular, smooth and 2–3 mm in diameter (after 24 h of
cultivation). Fluorescein is produced on King B medium. Pyocyanine is not produced
on King A medium.Strictly aerobic, optimum growth temperature 26 °C, min. 1 °C,
max. 34 °C.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar / Nutrient broth, Fermi's,
Cohn's & Uschinsky's solutions
cultivation). Fluorescein is produced on King B medium. Pyocyanine is not produced
on King A medium.Strictly aerobic, optimum growth temperature 26 °C, min. 1 °C,
max. 34 °C.
Media: Trypticase Soy Agar ± 5% sheep blood, Nutrient agar / Nutrient broth, Fermi's,
Cohn's & Uschinsky's solutions
Isolated from soil & plants (ferns - Asplenium nidus).
Fern pathogen - causes bacterial leaf blight of Asplenium nidus.
- Ark P.A. & Tompkins, CM (1946). "Bacterial leaf blight of bird's-nest fern". Phytopathology 36: 758-761.
- Savulescu T.: Contribution à la classification des bacteriacées phytopathogènes. Analele Academiei Romane Series III Tom
22, Memoire, 1947, 4, 1-26. - Tvrzova L., Schumann P., Sproer C., Sedlacek I., Pacova Z., Sedo O., Zdrahal Z., Steffen M. & Lang E.: Pseudomonas
moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds & emended
description of Pseudomonas asplenii. Int. J. Syst. Evol. Microbiol., 2006, 56, 2657-2663.
(c) Costin Stoica
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