|
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Proteobacteria => Gammaproteobacteria => Pasteurellales => Pasteurellaceae => Actinobacillus => Actinobacillus
pleuropneumoniae Pohl, Bertschinger, Frederiksen and Mannheim 1983.
Biovar 1 (V-factor-dependent strains previously classified as H. pleuropneumoniae;14 serovars) & Biovar 2 (V-factor-independent
strains).
Synonyms: Haemophilus pleuropneumoniae Shope 1964.
pleuropneumoniae Pohl, Bertschinger, Frederiksen and Mannheim 1983.
Biovar 1 (V-factor-dependent strains previously classified as H. pleuropneumoniae;14 serovars) & Biovar 2 (V-factor-independent
strains).
Synonyms: Haemophilus pleuropneumoniae Shope 1964.
Gram negative cells, nonmotile, nonsporeforming, small, and coccoid to rod-shaped,
occurring singly, in pairs, or in short chains.
occurring singly, in pairs, or in short chains.
Colonies are grayish white and smooth on chocolate agar; 3 mm in diameter after 48
hours in candle jar at 37 °C.
Beta hemolysis, but may be very slight or absent. CAMP test is positive.
V-factor dependency is variable ( Biovar 1 (V-factor-dependent strains previously
classified as H. pleuropneumoniae) & Biovar 2 (V-factor-independent strains)).
Aerobic, microaerophilic in primary isolation , facultatively anaerobic; no additional
CO2 is required for growth in subculture.
Mesophilic. Chemoorganotrophic , with pronounced growth response to fermentable
carbohydrates in peptone media.
Growth on MacConkey agar is negative.
hours in candle jar at 37 °C.
Beta hemolysis, but may be very slight or absent. CAMP test is positive.
V-factor dependency is variable ( Biovar 1 (V-factor-dependent strains previously
classified as H. pleuropneumoniae) & Biovar 2 (V-factor-independent strains)).
Aerobic, microaerophilic in primary isolation , facultatively anaerobic; no additional
CO2 is required for growth in subculture.
Mesophilic. Chemoorganotrophic , with pronounced growth response to fermentable
carbohydrates in peptone media.
Growth on MacConkey agar is negative.
Biovar 1 is causes 'porcine pleuropneumonia', a highly contagious respiratory
infection.
Most efficient antibiotics against A. pleuropneumoniae strains isolated from
diseased piglets in Romania were ampicillin, amoxicillin & gentamicin (100% of the
tested strains were susceptible to this antibiotics).
infection.
Most efficient antibiotics against A. pleuropneumoniae strains isolated from
diseased piglets in Romania were ampicillin, amoxicillin & gentamicin (100% of the
tested strains were susceptible to this antibiotics).
Biovar 1 is cause of porcine pleuropneumonia, a highly contagious respiratory infection. This severe and often fatal (fibrinous)
hemorrhagic bronchopneumonia leds to significant economic losses.
A. pleuropneumoniae produces three RTX toxins (Apx I,II,III), transferrin-binding proteins and urease. Other virulence factors: capsular
polysaccharide, cellular lipopolysaccharide and outer membrane proteins.
hemorrhagic bronchopneumonia leds to significant economic losses.
A. pleuropneumoniae produces three RTX toxins (Apx I,II,III), transferrin-binding proteins and urease. Other virulence factors: capsular
polysaccharide, cellular lipopolysaccharide and outer membrane proteins.
- Olsen I., MØller K., 2004.Genus II. Actinobacillus . In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part
B, George M. Garrity (Editor-in-Chief), pp. 866-883. - W.M. Janda, R. Mutters , 2006. Pasteurella, Mannheimia, Actinobacillus, Eikenella, Kingella, Capnocytophaga, and other
miscellaneous Gram-negative rods. In: Topley & Wilson’s Microbiology and Microbial Infections, 10 edition, Vol. 2,
Bacteriology, Edward Arnold Ltd. - J. G.Holt et al., 1994.Begey’s manual of Determinative Bacteriology, 9-edition, Williams & Wilkins.
- Sorescu I., 2002, Pleuropneumonia infectioasa a porcului: date recente privind etiopatogeneza si imunoprofilaxia. Buletin
informativ in patologie porcina, V, 1, 48-50. - Sorescu I. ,Stoica C., Drăghici D.,Stănuică D., 2001, Sensibilitatea la antibiotice si chimioterapice a tulpinilor de E. coli,
Salmonella choleraesuis grup C, Pasteurella multocida si Actinobacillus pleuropneumoniae izolate de la porcine in intervalul
aprilie 2001 - septembrie 2001. Buletin informativ in patologie porcina, IV, 1, 23-27. - Sorescu I., Stănuică D., Luminiţa Romaşcu, Vyan Zangana, 2002. Sensibilitatea la antibiotice şi chimioterapice a tulpinilor de
Escherichia coli, Salmonella choleraesuis grup C, Pasteurella multocida, Mannheimia spp. (Pasteurella haemolytica),
Actinobacillus pleuropneumoniae, Haemophilus parasuis şi Streptococcus spp. izolate de la porcine în intervalul Ian-Oct
2002, Buletin informativ in patologie porcina, V, 4, 3-8.
Nitrates reduction, alkaline phosphatase, H2S, D(+) xylose, mannitol, D(-) fructose,
urease, D(+) galactose, D(+) mannose, ONPG, maltose, sucrose (saccharose) &
D(+) glucose = positive.
urease, D(+) galactose, D(+) mannose, ONPG, maltose, sucrose (saccharose) &
D(+) glucose = positive.
Voges-Proskauer reaction, arginine dehydrolase, lysine decarboxylase, ornithine
decarboxylase, indole, glycerol, L(+) arabinose, dulcitol, meso-inositol, D(-) sorbitol,
L(+) rhamnose, L(-) sorbose, cellobiose, melibiose, trehalose, inulin, esculin, salicin
& gas from D(+) glucose = negative.
Oxidase, catalase, lactose & raffinose = variable.
decarboxylase, indole, glycerol, L(+) arabinose, dulcitol, meso-inositol, D(-) sorbitol,
L(+) rhamnose, L(-) sorbose, cellobiose, melibiose, trehalose, inulin, esculin, salicin
& gas from D(+) glucose = negative.
Oxidase, catalase, lactose & raffinose = variable.
| Actinobacillus pleuropneumoniae dependent of V factor (NAD) - producing Staphylococcus |
(c) Costin Stoica
| Antibiogram |
| Encyclopedia |
| Culture media |
| Biochemical tests |
| Stainings |
| Images |
| Movies |
| Articles |
| Identification |
| Software |
| R E G N U M PROKARYOTAE |
