| Listeria seeligeri |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Listeriaceae, Genus Listeria, Listeria seeligeri Rocourt and Grimont 1983.
L. seeligeri has 4 serovars (1/2b, 4c, 4d,6b), with 9 somatic (O factor) antigens (I, II, III, V, VI, VII, VIII, IX, XXI) and 3 flagella (H factor)
antigens (A,B,C).
Historical synonyms: Genomic group 5 of L. monocytogenes.
L. seeligeri has 4 serovars (1/2b, 4c, 4d,6b), with 9 somatic (O factor) antigens (I, II, III, V, VI, VII, VIII, IX, XXI) and 3 flagella (H factor)
antigens (A,B,C).
Historical synonyms: Genomic group 5 of L. monocytogenes.
Gram-positive short rods or coccobacilli, 0.4-0.8 x 0.5-2.5 µm, with rounded ends.
Coccoid forms are sometimes seen in smears from liquid cultures. Usually occur
singly or in short chains, palisades or diplo. Nonspore-forming. Motile with 2-6
peritrichous flagella when cultured at 28 ºC. Expression of the structural gene for the
flagellin protein (flaA) has been shown to be temperature regulated and repressed at
37 ºC. High-level expression is seen at 25 ºC, corresponding to the temperature at
which tumbling motility is observed.
Coccoid forms are sometimes seen in smears from liquid cultures. Usually occur
singly or in short chains, palisades or diplo. Nonspore-forming. Motile with 2-6
peritrichous flagella when cultured at 28 ºC. Expression of the structural gene for the
flagellin protein (flaA) has been shown to be temperature regulated and repressed at
37 ºC. High-level expression is seen at 25 ºC, corresponding to the temperature at
which tumbling motility is observed.
Grow well on blood agar base, nutrient, tryptose, and tryptose soy or brain heart
infusion agars; growth is enhanced by the addition of 0.2–1% (w/v) glucose, blood
or serum. Colonies on tryptose agar are small (1 to 2 mm diameter after 1 or 2 days of incubation at 37 ºC), smooth with a blue-
green color when they are examined with obliquely transmitted light. Beta-hemolytic; the zone of hemolysis is narrow and may be
detected only by removal of the colony from the agar surface.
CAMP test is negaitive with Rhodococcus equi and positive with Staphylococcus aureus.
Facultatively anaerobic. Grow at/on: pH 5.5-9.0. Variable growth in peptone water plus 10% NaCl. Optimum growth temperature is 37
ºC. Can grow at 4 ºC in 5 days. Multiply rapidly in milk.
infusion agars; growth is enhanced by the addition of 0.2–1% (w/v) glucose, blood
or serum. Colonies on tryptose agar are small (1 to 2 mm diameter after 1 or 2 days of incubation at 37 ºC), smooth with a blue-
green color when they are examined with obliquely transmitted light. Beta-hemolytic; the zone of hemolysis is narrow and may be
detected only by removal of the colony from the agar surface.
CAMP test is negaitive with Rhodococcus equi and positive with Staphylococcus aureus.
Facultatively anaerobic. Grow at/on: pH 5.5-9.0. Variable growth in peptone water plus 10% NaCl. Optimum growth temperature is 37
ºC. Can grow at 4 ºC in 5 days. Multiply rapidly in milk.
Widely distributed in nature, found in soil, mud, sewage, vegetation, and in the feces
of animals and man. Grow in the presence of 0.025% (w/v) thallous acetate; 3.75%
(w/v) potassium thiocyanate; 0.04% (w/v) potassium tellurite and in the presence of
10 µg/ml tripaflavine. Do not grow in the presence of 0.02% (w/v) sodium azide.
It is usually sensitive to amikacin, amoxycillin, ampicillin, azlocillin, ciprofloxacin,
chloramphenicol, clindamycin coumermycin, doxycycline, enoxacin, erythromycin,
gentamicin, imipen, netilmicin, penicillin, rifampin, trimethoprim, and vancomycin.
Resistant to the cephalosporins, phosphomycin, and polymyxin.
of animals and man. Grow in the presence of 0.025% (w/v) thallous acetate; 3.75%
(w/v) potassium thiocyanate; 0.04% (w/v) potassium tellurite and in the presence of
10 µg/ml tripaflavine. Do not grow in the presence of 0.02% (w/v) sodium azide.
It is usually sensitive to amikacin, amoxycillin, ampicillin, azlocillin, ciprofloxacin,
chloramphenicol, clindamycin coumermycin, doxycycline, enoxacin, erythromycin,
gentamicin, imipen, netilmicin, penicillin, rifampin, trimethoprim, and vancomycin.
Resistant to the cephalosporins, phosphomycin, and polymyxin.
Not pathogenic for mice, other animals and man.
- Hammes W.P. and Hertel C., 2009. Genus I. Listeria Pirie 1940. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 244-
257. - McLauchlin J., 2005. Listeria. In: Topley & Wilson’s Microbiology & Microbial Infections, 10th Edition, Edited by Borriello S.P.,
Murray P.R. and Funke G., 953-969. - Khelef N., Lecuit M., Buchrieser C., Cabanes D., Dussurget O. and Cossart P., 2006. Listeria monocytogenes and the genus
Listeria. In: Dworkin M., Falkow S., Rosenberg E., Schleifer K.H., Stackebrandt (Editors), The Prokaryotes, A Handbook on the
Biology of Bacteria, Third Edition, Volume 4, Bacteria: Firmicutes, Cyanobacteria, Springer, Chapter 1.2.11, 404-476. - Rocourt J. and Grimont P.A.D., 1983. Listeria welshimeri sp. nov. and Listeria seeligeri sp. nov. IJSB Vol. 33, No. 4, p. 866-869.
- Den Bakker, H. C., Warchocki, S., Wright, E. M., Allred, A. F., Ahlstrom, C., Manuel, C. S., Stasiewicz, M. J., Burrell, A., Roof, S.,
Strawn, L. K., Fortes, E., Nightingale, K. K., Kephart, D. and Wiedmann, M. 2014. Listeria floridensis sp. nov., Listeria aquatica sp.
nov., Listeria cornellensis sp. nov., Listeria riparia sp. nov. and Listeria grandensis sp. nov., from agricultural and natural
environments. Int. J. Syst. Evol. Microbiol., 64, 1882-1889.
Positive results for arylesterase activity, catalase, chymotrypsin, esculin hydrolysis, N-acetyl-beta-glucosamidase, leucine
esterase,methyl-red, acid phosphatase, phosphoamidase, Voges-Proskauer reaction, amino acid peptidase: D-alanine & lysine;
acid production from: esculin, D-glucose, glycerol, 5-ketogluconate, lactose, melezitose, methyl alpha-D-glucoside, salicin, trehalose,
turanose, xylitol & D-xylose.
Negative results for casein hydrolysis, cystine arylamidase, citrate utilization, gelatin hydrolysis, beta-glucosidase, indole production,
H2S production, nitrates reduction to nitrites, urea hydrolysis, oxidase, amino acid peptidase: ornithine, glutamic acid & arginine; acid
production from: L-arabinose, galactose, glucose 1-phosphate, glycogen, D-lyxose, D-mannitol, melibiose, methyl
alpha-D-mannoside, rhamnose, ribose & tagatose.
Variable results for alpha-glucosidase, lecithinase, starch hydrolysis, tween 80 esterase, acid production from: gluconate, soluble
starch & sucrose.
esterase,methyl-red, acid phosphatase, phosphoamidase, Voges-Proskauer reaction, amino acid peptidase: D-alanine & lysine;
acid production from: esculin, D-glucose, glycerol, 5-ketogluconate, lactose, melezitose, methyl alpha-D-glucoside, salicin, trehalose,
turanose, xylitol & D-xylose.
Negative results for casein hydrolysis, cystine arylamidase, citrate utilization, gelatin hydrolysis, beta-glucosidase, indole production,
H2S production, nitrates reduction to nitrites, urea hydrolysis, oxidase, amino acid peptidase: ornithine, glutamic acid & arginine; acid
production from: L-arabinose, galactose, glucose 1-phosphate, glycogen, D-lyxose, D-mannitol, melibiose, methyl
alpha-D-mannoside, rhamnose, ribose & tagatose.
Variable results for alpha-glucosidase, lecithinase, starch hydrolysis, tween 80 esterase, acid production from: gluconate, soluble
starch & sucrose.
(c) Costin Stoica
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