Genus Pragia
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Proteobacteria, Class Gammaproteobacteria, Order Enterobacteriales, Family Enterobacteriaceae, Genus Pragia, Pragia
fontium
- type species of the genus, Aldova et al. 1988.
Gram-negative rods. Motile by peritrichous flagella.
Nonhemolytic colonies on sheep blood agar, 0.5 mm in diameter. Facultatively
anaerobic,  optimum growth temperature 30 ºC. Growth at 4 ºC, but not at 42 ºC.
Grow on media: Nutrient agar or nutrient broth (some strains produce a Shigella-like
odour on nutrient agar), Endo, Mac-Conkey agar, Trypticase Soy Agar ± 5% sheep
blood, TY broth (8 g casein enzymatic hydrolysate type I, 5 g yeast extract powder, 5 g
NaCl per 1,000 ml H
2O).
Isolated from water (17 strains) & feces (1 strain).
Unknown pathogenicity (non-pathogenic).
  1. J. G.Holt et al., 1994. Begey’s manual of Determinative Bacteriology, 9th-edition, Williams & Wilkins.1994.
  2. Aldova E., Hausner O., Brenner D.J., Kocmoud Z., Schindler J., Potuznikova B. & Petras P.: Pragia fontium gen. nov., sp. nov. of the
    family Enterobacteriaceae, isolated from water. Int. J. Syst. Bacteriol., 1988, 38, 183-189.
  3. Don J. Brenner & J.J. Farmer III,2004, Family I. Enterobacteriaceae, In:  Bergey’s Manual of Systematic Bacteriology, Second
    edition,Vol two, part B, George M. Garrity (Editor-in-Chief),   pp. 740-744.
Positive results for H2S production, catalase, nitrates reduction, citrate utilization &
methyl red test.

Negative results for oxidase, indole production, Voges–Proskauer test, phenylalanine deaminase, lysine and ornithine
decarboxylases, arginine dihydrolase, urease, deoxyribonuclease, malonate, ONPG, growth in KCN medium, acid production from L-
arabinose, cellobiose, dulcitol, lactose, maltose, D-mannitol, D-mannose, melibiose, raffinose, L-rhamnose, D-sorbitol, D-sorbose,
starch, sucrose & trehalose.
Acid production from glycerol and myo-inositol may be delayed-positive reaction.
(c) Costin Stoica
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