|Bacillus pumilus vegetative cells and central,
nondeforming, elipsoidal spores. Gram staining.
|Bacillus pumilus colonies on Sheep Blood Agar
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus pumilus Meyer and Gottheil (1901).
Gram-positive or Gram-variable, 2.0-3.0 x 0.6-0.7 μm, motile rods with peritrichous flagella. Ellipsoidal or cylindrical, central, paracentral
or subterminal spore, not deforming the vegetative cell. No capsule present.
Colonial morphology is variable; colonies may be wrinkled and irregular, and they are
unpigmented and most are opaque. The colonies of most strains on nutrient agar are
smooth and become slightly yellowish. Nonhemolytic on sheep blood agar, but two
hemolitic Gram-positive bacillus strain that biochemically was identified as Bacillus
pumilus both by 'API CHB' and 'ABIS online' were isolated from sheep milk and honey
(admin note). Aerobic, do not grow anaerobically. Growth at pH 5.7-9.5, some strains
will grow at pH 4.5. Grow in 0-10% NaCl. Temperature range: 5-15 ºC to 40-50 ºC.
Allantoin or urate are not required for growth.
Widely distributed in clinical (human faeces and ileum) and veterinary specimens,
food ingredients, emperor moth caterpillars, leather, paper. Spores are widely
distributed in nature (soil, plants).
Variable growth in the presence of lysozyme.
Isolates of Bacillus pumilus from Antarctic soils and penguin rookeries show some
phenotypic distinction from other strains of the species, including the production of a
diffusible yellow pigment by some strains on initial culture.
Food intoxications to humans: may produce toxins - a complex of lipopeptides
(pumiciladines). Rarely encountered. May be involved in rectal fistula.
Isolated from bovines with mastitis (rarely).
Caused widespread lysis and damage (including nuclear condensation and
fragmentation , cell lysis and necrosis, vacuolation and apoptosis) to HEp-2 cells.
Antifungal activity; used in agriculture.
- Bîlbîie V., Pozsgi N., 1985, Bacteriologie Medicală, vol.ll, Ed. Medicală, Bucureşti.
- Gordon R.E., Haynes W.C., Pang C.H. (1973) – The genus Bacillus . Agriculture Handbook No. 427, U.S.D.A., Washington D.C.
- Buchanan R.E., Gibbons N.E., Cowan S.T., Holt J.G., Liston J., Murray R.G.E., Niven C.F., Ravin A.W., Stanier R.W. ( 1974) –
Bergey’s Manual of Determinative Bacteriology, Eight Edition, The Williams & Wilkins Company, Baltimore.
- Bottone, Edward J; Peluso, Richard W: Production by Bacillus pumilus (MSH) of an antifungal compound that is active against
Mucoraceae and Aspergillus species: preliminary report. Journal of Medical Microbiology. 52(1):69-74, January 2003.
- Buiuc D., Negut M. , 1999. Tratat de Microbiologie Clinica, Editura Medicala, Bucuresti.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Hoyles L., Honda H., Logan N.A., Halket G., La Ragione R., McCartney A.L., 2012. Recognition of greater diversity of Bacillus
species and related bacteria in human faeces. Research in Microbiology 163, 3-13.
Positive results for beta-galactosidase, Voges-Proskauer, citrate utilization, hydrolysis
of esculin, hydrolysis of gelatin, hydrolysis of casein, acid production from: N-acetil-D-
glucosamine, L-arabinose, amygdalin, arbutin, D-cellobiose, D-fructose, galactose,
glucose, glycerol, beta-gentibiose, D-mannose, D-raffinose, ribose, sucrose, salicin,
trehalose, & D-xylose.
Negative results for reduction of nitrate to nitrite, starch hydrolysis, indole, hippurate,
arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, tryptophan
deaminase, egg yolk reaction, phenylalanine deaminated, degradation of tyrosine,
acid production from: D-arabinose, adonitol, D- or L-arabitol, glycogen, methyl
beta-xyloside, starch, dulcitol, erythritol, D- or L-fucose, inulin, 2- or 5-ketogluconate,
gluconate, lyxose, D-melezitose, rhamnose, sorbose, xylitol & L-xylose.
Variable results for oxidase, acid production from: meso-inositol, lactose, maltose,
D-melibiose & sorbitol.
(c) Costin Stoica