Strain / code
ABIS 6 online *
Apiweb
( Biomerieux)
Comments
Streptococcus uberis ATCC 700407
S. uberis 75% (18)**
S. uberis 99% (20)
 
Bacillus polymyxa ATCC 43865
B. polymyxa 98% (16)
B. polymyxa 97% (49)
 
Streptococcus equi subsp.
zooepidemicus ATCC 700400
S. equi subsp.
zooepidemicus 89% (20)
S. equi subsp.
zooepidemicus 99% (20)
 
Providencia alcalifaciens ATCC  9886
P. alcalifaciens 91% (29)
P. alcalifaciens 99% (60)
 
Escherichia coli ATCC 25922
E. coli 89% (22)
E. coli 99% (22)
 
Enterobacter cloacae
ATCC 13047
E. cloacae 92% (20)
Enterobacter cloacae
95% (22)
 
Proteus mirabilis ATCC 35659
P. mirabilis 94% (20)
P. mirabilis 99% (22)
 
Klebsiella pneumoniae ssp.
pneumoniae ATCC 35657
K. pneumoniae ssp.
pneumoniae 98% (21)
K. pneumoniae ssp.
pneumoniae 97% (22)
 
Pseudomonas aeruginosa
ATCC 27853
P. aeruginosa 95% (8)
P. aeruginosa 99% (21)
 
Bacillus anthracis 1190 R
B. anthracis 98% (18)
  Not tested in API
Salmonella choleraesuis 014
Salmonella choleraesuis
salamae 90% (25)
Salmonella choleraesuis
99% (24)
Salm. group C
confirmed serologically
Staphylococcus lentus ATCC 700403
S. lentus 76% (20)
S. lentus 99% (20)
 
Staphylococcus xylosus ATCC 700404
S. nepalensis 92%
S. xylosus 90% (17)
S. xylosus 99% (19)
S. nepalensis is not
identifiable by Apiweb
Staphylococcus capitis ATCC 35661
S.capitis 89% (19)
S. capitis 99% (21)
 
Collection strains
* ABIS tested in normal mode. Higher percents resulted when using forced identification.
** Number of used tests. Not all available test were used by both identification systems; some tests are included in
ABIS 6 online
identification scheme and not included in
Apiweb; and vice versa.
Comparative bacteria identification results of ABIS 6 online
and API (Biomerieux) software
Custom Search
Strain / code
ABIS 6 online
Apiweb
( Biomerieux)
Comments
2826 dog otitis
Staphylococcus schleiferi subsp.
coagulans 85% (23)
Staphylococcus schleiferi
44% (20)
 
  Citrobacter freundii 99% (20)
Citrobacter freundii (22)
API unaccepted profile
040905
Enterobacter cloacae 92% (24)
Enterobacter cloacae (22)
API unaccepted profile
3338
Serratia ficaria 88%
S. marcescens 83% (18)
S. marcescens (22)
API unaccepted profile
2946 feed
Salmonella choleraesuis
houtenae 92% (22)
Salmonella spp. (22)
API unaccepted profile
Salm. group F
confirmed serologically
8943 feed
Salmonella choleraesuis
houtenae 97% (23)
Salmonella spp. (22)
Salmonella arizonae
API unaccepted profile
Salm. group E
confirmed serologically
10910 feed
Salmonella choleraesuis
choleraesuis 97% (23)
Salmonella spp. (22)
API unaccepted profile
Salm. group D
confirmed serologically
3043
Citrobacter braaki
C. freundii 96% (22)
Citrobacter braaki
C. freundii (22)
API unaccepted profile
3160
Morganella morganii 98% (23)
Morganella morganii 99% (23)
 
wild swine liver
Salmonella choleraesuis
houtenae 92% (22)
Salmonella spp. (24)
API unaccepted profile
Salm. group D
confirmed serologically
4753 swine lungs
Salmonella choleraesuis
salamae 85% (22)
Salmonella choleraesuis
99% (22)
Salm. group C
confirmed serologically
197
Providencia rettgeri 92%
P. stuartii 79% (24)
Providencia rettgeri
P. stuartii  (24)
API unaccepted profile
5276
Salmonella choleraesuis
salamae 86% (25)
Salmonella spp.
S. paratyphi A
API unaccepted profile
Salm. group D
confirmed serologically
6032
Salmonella choleraesuis
salamae 86% (25)
Salmonella choleraesuis
99% (24)
Salm. group C
confirmed serologically
7166
Enterobacter cloacae 88% (24)
Enterobacter cloacae  (24)
API unaccepted profile
834 swine liver
Salmonella choleraesuis
houtenae 85% (24)
Salmonella spp. 48% (24)
Salm. group B
confirmed serologically
canary leg necrosis
Klebsiella pneumoniae subsp.
pneumoniae 95% (25)
K. pneumoniae subsp.
pneumoniae 98% (24)
 
1272 pork meat
Proteus mirabilis 91% (25)
Proteus mirabilis 99% (24)
 
7292
Pseudomonas aeruginosa
84% (8)
Pseudomonas aeruginosa
98% (21)
 
10765 dog urinary
infection
Chryseomonas luteola
91% (10)
Chryseomonas luteola
99% (21)
 
10241 cat skin
Staphylococcus aureus
93% (17)
Staphylococcus aureus
88% (20)
 
B
Staphylococcus epidermidis
92% (24)
Staphylococcus epidermidis
97% (20)
 
5997
Staphylococcus carnosus
subsp. carnosus 88% (19)
Staphylococcus carnosus (20)
API unaccepted profile
7983 M2/4
Staphylococcus aureus subsp.
aureus 94% (20)
Staphylococcus aureus
98% (20)
 
7292
Staphylococcus haemolyticus  
91% (19)
Staphylococcus haemolyticus  
86% (20)
 
7066
Staphylococcus simulans
86% (23)
Staphylococcus simulans  96%
(20)
 
3460
Staphylococcus haemolyticus  
91% (21)
S. lugdunensis 88%
S. haemolyticus  37% (20)
S. lugdunensis 27%
 
5005
Staphylococcus aureus subsp.
aureus 93% (20)
Staphylococcus aureus
88% (20)
 
3483
Staphylococcus aureus subsp.
aureus 98% (19)
Staphylococcus aureus
90% (20)
 
1577
Staphylococcus intermedius
97% (23)
Staphylococcus aureus
(S. intermedius) 66% (20)
 
1122M
Staphylococcus intermedius
97% (24)
Staphylococcus aureus
(S. intermedius) 88% (20)
 
468
Staphylococcus intermedius
97% (24)
S. chromogenes 90%
S. aureus (S. intermed.) 8% (20)
Coagulase positive &
non-pigmented strain
1989 dog otitis
Enterobacter cloacae 89% (25)
Enterobacter cloacae
95% (25)
 
85 swine lungs
Streptococcus dysgalactiae
subsp. equisimilis 82% (20)
Streptococcus dysgalactiae
subsp. equisimilis 87% (20)
 
  Streptococcus bovis 96% (21)
Strept. bovis II 67% (21)
 
7844
Pasteurella multocida
79% (10)
Pasteurella multocida
92% (20)
 
15040
Streptococcus dysgalactiae
subsp. equisimilis 86% (20)
Streptococcus dysgalactiae
subsp. equisimilis 98% (20)
 
dog skin
Staphylococcus aureus subsp.
aureus 89% (22)
Staphylococcus aureus
92% (20)
 
3879 dog conjunctivitis
Enterobacter amnigenus 2
96%
Enterobacter amnigenus 2
96%
 
4209 cat vaginal secretion
Chryseomonas luteola
91% (8)
Chryseomonas luteola
(21)
API unaccepted profile
5265 sanitation test swine
Stenotrophomonas maltophilia
91% (10)
Stenotrophomonas maltophilia
45% (21)
 
3269 pigeon leg arthritis
Salmonella choleraesuis
salamae 90% (24)
Salmonella spp. 85% (24)
Salm. group B
confirmed serologically
6528 RV feed
Proteus mirabilis 94% (23)
Proteus mirabilis 99% (23)
 
6528 KM feed
Enterobacter cloacae 96% (23)
Enterobacter cloacae 89% (23)
 
6860 chicken liver
Staphylococcus aureus subsp.
aureus 94% (23)
Staphylococcus aureus subsp.
aureus 98% (20)
 
6075 pig feces
Salmonella enterica subsp.
enterica 90% (24)
Salmonella spp. 99% (23)
Salm. group B
confirmed serologically
Wild strains
Presented results show that ABIS 6 online is capable of correct taxon identification. In most of cases
results were identical with those found by Apiweb. Sometimes identification probability  was 5-10%
lower than Apiweb when used Normal mode and 5% higher or equal when used Forced
identification. Forced mode was used only when program suggested it, in case of insufficient tests
available.

Identification of collection strains was similar to Apiweb; few questionable results were obtained
when testing wild strains, some of them were confirmed serologically (Salmonella) or with
supplementary tests (Staphylococcus intermedius vs. S. chromogenes).

In cases where Apiweb was unable to validate its results because of the "Unacceptable profile", we
were happy to observe that ABIS confirmed Apiweb's results with a high id%, suggesting that both
programs made a good taxon identification.
14 collection and 46 wild bacteria strains were tested with ABIS 6 online & Apiweb software using all laboratory
available tests and API galleries. Results are presented below.
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