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PROKARYOTAE
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(c) Costin Stoica
Strain name and code
ABIS results id%
apiweb™ results id%
Bacillus megaterium
NCIMB 8508
Bacillus megaterium 90%
Bacillus megaterium 99.6%
Bacillus thuringiensis
NCIMB 9134
Bacillus thuringiensis 93%
Bacillus cereus, possibility of B.
thuringiensis 98.9%
Clostridium tetani
RO-2009
Clostridium tetani 97%
Clostridium tetani (no id%)
Enterococcus faecium
NCIMB 11181
Enterococcus faecium 92%
Enterococcus faecium 99.2%
Enterococcus faecium
NCIMB 10415
Enterococcus faecium 97%
Enterococcus faecium 93.9%
Escherichia coli
ATCC 8739
Escherichia coli 98%
Escherichia coli 99.9%
Helicobacter pylori
ATCC 43504
Helicobacter pylori 94%
Helicobacter pylori 99.9%
Lactobacillus plantarum
ATCC 8014
Lactobacillus plantarum 91%
Lactobacillus plantarum 1 99.9%
Lactobacillus paracasei
CCM 1837
Lactobacillus paracasei subsp.
paracasei  94%
Lactobacillus paracasei subsp.
paracasei 1 / 3  48.0 / 51.0%
Proteus hauseri
ATCC 13315
Proteus vulgaris / P. hauseri  95%
Proteus vulgaris group 99.8%
Pseudomonas aeruginosa
ATCC 15442
Pseudomonas aeruginosa 92%
Pseudomonas aeruginosa 98.1%
Salmonella Choleraesuis
RO-014
Salmonella spp. 87%
Salmonella choleraesuis 99.7%
Salmonella Typhimurium
ATCC 14028
Salmonella spp. 94%
Salmonella spp. 99.9%
Salmonella Typhimurium
RO-TM2
Salmonella spp. 89%
Salmonella spp. 99.0%
Staphylococcus aureus
ATCC 25293
Staphylococcus aureus subsp.
aureus 99%
Staphylococcus aureus 97.8%
Streptococcus mutans
ATCC 55677
Streptococcus mutans 98%
Streptococcus mutans 99.9%
Comparative reference strains identification results of
'ABIS online' and 'apiweb™' software
Legend:
* ABIS %id represents the percentage of similarity with taxa from the database, all databases containing a matrix where
probabilistic incidence values are allocated for every taxon and their corresponding morpho-biochemical characters;
** apiweb™ %id is a probabilistic calculation using  bioMerieux own system procedure.
The morphological and biochemical characters of 16 reference strains were tested by ABIS online and apiweb™ software (bioMerieux)
using all laboratory available tests (classical tests and API bioMerieux strips). The reference strains were derived from ROMVAC
Company and Cantacuzino Institute bacterial collections.
Discussions
Very closed results (same taxa, with low difference, ≤ 10%, in id%) were obtained for reference strains in comparative identification with
ABIS online and apiweb™ software.
For the reference strain
Clostridium tetani RO-2009 apiweb™ returned Clostridium tetani without calculating an id%, as an alternative
result after
Egerthella lenta 75%, Clostridium spp. 16.9% and Clostridium hystolyticum 3%, while ABIS result was Clostridium tetani
97%.
Proteus hauseri is biochemically similar to strains commonly identified as Proteus vulgaris, but esculin and salicin negative. Since
these tests were not available neither in lab, nor in API galleries, the ATCC 13315 -
Proteus hauseri strain was identified by ABIS as “P.
vulgaris / P. hauseri
”, and by apiweb™ as “P. vulgaris group”.

Conclusion
ABIS online returned similar results with apiweb™, with a high id%, confirming the reference strains and proving very good taxa
identification.